, Volume 64, Issue 6, pp 665–676 | Cite as

Diversity and abundance of natural enemies of Drosophila suzukii in Wisconsin, USA fruit farms

  • Matthew T. Kamiyama
  • Zachary Schreiner
  • Christelle GuédotEmail author
Original Article


The invasive vinegar fly, Drosophila suzukii (Matsumura) (Diptera: Drosophilidae), is a major economic pest of soft-skinned fruit in the USA. In order to identify suitable biological control agents in Wisconsin, we assessed the presence and impact of potential natural enemies of D. suzukii in tart cherry and raspberry farms with documented D. suzukii populations. Yellow sticky cards and sentinel traps baited with D. suzukii or D. melanogaster larvae and pupae were deployed in these agricultural areas in the foliage and near the soil surface. Four families of parasitoids with known species that successfully parasitize D. suzukii (Pteromalidae, Braconidae, Figitidae, and Diapriidae) were found on sticky traps at all sampling sites. However, no parasitoids emerged from D. suzukii or D. melanogaster sentinel larvae or pupae throughout the duration of the field assessment. Predation was documented on sentinel D. suzukii pupae (1–4%) and sentinel D. melanogaster pupae (5–8%). The majority of sentinel pupae never emerged upon their return from the field and showed no evidence of field predation. Between 70 and 92% of recovered D. suzukii pupae were dead, and 46–72% of D. melanogaster pupae were dead. The absence of parasitoids found emerging from the sentinel drosophilid larvae and pupae and overall low levels of predation suggests that native biological control may provide limited potential as a management option against D. suzukii in Wisconsin at this time.


Spotted-wing Drosophila Sentinel trap Predation Non-reproductive effects Pseudoparasitism 



We give special thanks to all participating growers for access to their farms. We thank Janet van Zoeren, Benjamin Jaffe, Samuel DeGrey, and Michaela Taddeini for their help with data processing and field work. This project was funded by the Wisconsin Department of Agriculture, Trade, and Consumer Protection Specialty Crop Block Grant # 16-01.

Compliance with ethical standards

Conflict of interest

The authors do not report conflicts of interest.

Supplementary material

10526_2019_9966_MOESM1_ESM.docx (23 kb)
Supplementary material 1 (DOCX 23 kb)


  1. Abram PK, Brodeur J, Urbaneja A, Tena A (2019) Nonreproductive effects of insect parasitoids on their hosts. Annu Rev Entomol 64:1–15Google Scholar
  2. Arnó J, Solà M, Riudavets J, Gabarra R (2016) Population dynamics, non-crop hosts, and fruit susceptibility of Drosophila suzukii in Northeast Spain. J Pest Sci 89:713–723Google Scholar
  3. Asplen MK, Anfora G, Biondi A, Choi DS, Chu D, Daane KM, Gibert P, Gutierrez AP, Hoelmer KA, Hutchinson WD, Isaacs R, Jiang ZL, Kárpáti Z, Kimura MT, Pascual M, Philips CR, Plantamp C, Ponti L, Vétek G, Vogt H, Walton VM, Yu Y, Zappalá L, Desneux N (2015) Invasion biology of spotted wing Drosophila (Drosophila suzukii): a global perspective and future priorities. J Pest Sci 88:469–494Google Scholar
  4. Beers EH, van Steenwyk RA, Shearer PW, Coates WW, Grant JA (2011) Developing Drosophila suzukii management programs for sweet cherry in the western United States. Pest Manag Sci 67:1386–1395PubMedGoogle Scholar
  5. Bolda M, Goodhue RE, Zalom FG (2010) Spotted wing Drosophila: potential economic impact of a newly established pest. Agric Resour Econ 13:5–8Google Scholar
  6. Bruck DJ, Bolda M, Tanigoshi L, Klick J, Kleiber J, DeFrancesco J, Gerdeman B, Spitler H (2011) Laboratory and field comparisons of insecticides to reduce infestation of Drosophila suzukii in berry crops. Pest Manag Sci 67:1375–1385PubMedGoogle Scholar
  7. Chabert S, Allemand R, Poyet M, Eslin P, Gibert P (2012) Ability of European parasitoids (Hymenoptera) to control a new invasive Asiatic pest, Drosophila suzukii. Biol Control 63:40–47Google Scholar
  8. Daane KM, Wang XG, Biondi A, Miller B, Miller JC, Riedl H, Shearer PW, Guerrieri E, Giorgini M, Buffington M, van Achterberg K, Song Y, Kang T, Yi H, Jung C, Lee DW, Chung BK, Hoelmer KA, Walton VM (2016) First exploration of parasitoids of Drosophila suzukii in South Korea as potential classical biological agents. J Pest Sci 89:823–835Google Scholar
  9. Dancau T, Stemberger TL, Clarke P, Gillespie DR (2017) Can competition be superior to parasitism for biological control? The case of spotted wing Drosophila (Drosophila suzukii), Drosophila melanogaster and Pachycrepoideus vindemmiae. Biocontrol Sci Technol 27:3–16Google Scholar
  10. Diepenbrock LM, Burrack HJ (2016) Variation of within-crop microhabitat use by Drosophila suzukii (Diptera: Drosophilidae) in blackberry. J Appl Entomol 141:1–7Google Scholar
  11. Digiacomo G, Hadrich J, Hutchison WD, Peterson H, Rogers M (2019) Economic impact of spotted-wing Drosophila (Diptera: Drosophilidae) yield loss on Minnesota raspberry farms: a grower survey. J Integr Pest Manag 10(1):11Google Scholar
  12. Fleury F, Ris N, Allemand R, Fouillet P, Carton Y, Boulétreau M (2004) Ecological and genetic interactions in Drosophila-parasitoids communities: a case study with D. melanogaster, D. simulans and their common Leptopilina parasitoids in south-eastern France. Genetica 120:181–194PubMedGoogle Scholar
  13. Gabarra R, Riudavets J, Rodríguez GA, Pujade-Villar J, Arnó J (2015) Prospects for the biological control of Drosophila suzukii. BioControl 60:331–339Google Scholar
  14. Girod P, Lierhmann O, Urvois T, Turlings TCJ, Kenis M, Haye T (2018) The parasitoid complex of D. suzukii and other fruit feeding Drosophila species in Asia. Sci Rep 8:11839PubMedPubMedCentralGoogle Scholar
  15. Goodhue RE, Bolda M, Farnsworth D, Williams JC, Zalom FG (2011) Spotted wing drosophila infestation in California strawberries and raspberries, economic analysis of potential revenue losses and control costs. Pest Manag Sci 67:1396–1402PubMedGoogle Scholar
  16. Guédot C, Avanesyan A, Hietala-Henschell K (2018) Effect of temperature and humidity on the seasonal phenology of Drosophila suzukii (Diptera: Drosophilidae) in Wisconsin. Environ Entomol 47:1365–1375PubMedGoogle Scholar
  17. Hamby KA, Bellamy DE, Chiu JC, Lee JC, Walton VM, Wiman NG, York RM, Biondi A (2016) Biotic and abiotic factors impacting development, behavior, phenology, and reproductive biology of Drosophila suzukii. J Pest Sci 89:605–619Google Scholar
  18. Hauser M (2011) A historic account of the invasion of Drosophila suzukii (Matsumura) (Diptera, Drosophilidae) in the continental United States, with remarks on their identification. Pest Manag Sci 67:1352–1357PubMedGoogle Scholar
  19. Hoelmer KA, Simmons AM (2008) Yellow sticky trap catches of parasitoids of Bemisia tabaci (Hemiptera: Aleyrodidae) in vegetable crops and their relationship to in-field populations. Environ Entomol 37:391–399PubMedGoogle Scholar
  20. Isaacs R (2011) First detection and response to the arrival of spotted wing drosophila in Michigan. Newsl Mich Entomol Soc 56:10–12Google Scholar
  21. Kacsoh BZ, Schlenke TA (2012) High hemocyte load is associated with increased resistance against parasitoids in Drosophila suzukii, a relative of D. melanogaster. PLoS ONE 7(4):e34721PubMedPubMedCentralGoogle Scholar
  22. Kaser JM, Nielsen AL, Abram PK (2018) Biological control effects of non-reproductive host mortality caused by insect parasitoids. Ecol Appl 28:1081–1092PubMedGoogle Scholar
  23. Knoll V, Ellenbroek T, Romeis J, Collatz J (2017) Seasonal and regional presence of hymenopteran parasitoids of Drosophila in Switzerland and their ability to parasitize the invasive Drosophila suzukii. Sci Rep 7:40697PubMedPubMedCentralGoogle Scholar
  24. Lee JC, Wang X, Daane KM, Hoelmer KA, Isaacs R, Sial AA, Walton VM (2019) Biological control of spotted-wing drosophila (Diptera: Drosophilidae)—current and pending tactics. J Integr Pest Manag 10:1–9Google Scholar
  25. Little CM, Chapman TW, Moreau DL, Hillier NK (2016) Susceptibility of selected boreal fruits and berries to the invasive pest Drosophila suzukii (Diptera: Drosophilidae). Pest Manag Sci 73:160–166PubMedGoogle Scholar
  26. Miller B, Anfora G, Buffington M, Daane KM, Dalton DT, Hoelmer K, Rossi Stacconi MV, Grassi A, Ioratti C, Loni A, Miller JC, Ouantar M, Wang XG, Wiman N, Walton VM (2015) Seasonal occurrence of resident parasitoids associated with Drosophila suzukii in two small fruit production regions of Italy and the USA. Bull Insectol 68:255–263Google Scholar
  27. New TR (1975) The biology of Chrysopidae and Hemerobiidae (Neuroptera), with reference to their usage as biocontrol agents: a review. Trans Entomol Soc Lond 127:115–140Google Scholar
  28. Pfeiffer DG, Wahls JCE (2016) Biological control agents for spotted wing drosophila in Virginia, Fourth Quarterly Report. Va Vineyards AssociationGoogle Scholar
  29. Poyet M, Havard S, Prevost G, Chabrerie O, Doury G, Gibert P, Eslin P (2013) Resistance of Drosophila suzukii to the larval parasitoids Leptopilina heterotoma and Asobara japonica is related to haemocyte load. Physiol Entomol 38:45–53Google Scholar
  30. Renkema JM, Telfer Z, Gariepy T, Hallett RH (2015) Dalotia coriaria as a predator of Drosophila suzukii: functional responses, reduced fruit infestation and molecular diagnostics. Biol Control 89:1–10Google Scholar
  31. Rossi Stacconi MV, Grassi A, Dalton DT, Miller B, Ouantar M, Loni A, Ioriatti C, Walton VM, Anfora G (2013) First field records of Pachycrepoideus vindemmiae as a parasitoid of Drosophila suzukii in European and Oregon small fruit production areas. Entomologia 1(e3):11–16Google Scholar
  32. Rossi Stacconi MV, Amiresmaeili N, Biondi A, Carli C, Caruso S, Dindo ML, Francati S, Gottardello A, Grassi A, Lupi L (2018) Host location and dispersal ability of the cosmopolitan parasitoid Trichopria drosophilae released to control the invasive spotted wing Drosophila. Biol Control 117:188–196Google Scholar
  33. Shaw B, Brain P, Wijnen H, Fountain MT (2017) Reducing Drosophila suzukii emergence through inter-species competition. Pest Manag Sci 74:1466–1471Google Scholar
  34. R Core Team (2018) R: a language and environment for statistical computing. R Foundation for Statistical Computing. Vienna.
  35. Tochen S, Dalton DT, Wiman NG, Hamm C, Shearer PW, Walton VM (2014) Temperature-related development and population parameters for Drosophila suzukii (Diptera: Drosophilidae) on cherry and blueberry. Environ Entomol 43:501–510PubMedGoogle Scholar
  36. Tonina L, Mori N, Giomi F, Battisti A (2016) Development of Drosophila suzukii at low temperatures in mountain areas. J Pest Sci 89:667–678Google Scholar
  37. van Timmeren S, Isaacs R (2013) Control of spotted wing drosophila, Drosophila suzukii, by specific insecticides and by conventional and organic crop protection programs. Crop Prot 54:126–133Google Scholar
  38. Walsh DB, Bolda MP, Goodhue RE, Dreves AJ, Lee J, Bruck DJ, Walton VM, O’Neal SD, Zalom FG (2011) Drosophila suzukii (Diptera: Drosophilidae): invasive pest of ripening soft fruit expanding its geographic range and damage potential. J Integr Pest Manag 2:1–7Google Scholar
  39. Wang XG, Kaçar G, Biondi A, Daane KM (2016) Life-history and host preference of Trichopria drosophilae, a pupal parasitoid of spotted wing drosophila. BioControl 61:387–397Google Scholar
  40. Wiman N, Walton VM, Dalton DT, Anfora G, Burrack HJ, Chiu JC, Daane KM, Grassi A, Miller B, Tochen S, Wang XG, Ioriatti C (2014) Integrating temperature-dependent life table data into a matrix projection model for Drosophila suzukii population estimation. PLoS ONE 9(9):e106909PubMedPubMedCentralGoogle Scholar
  41. Wolf S, Zeisler C, Sint D, Romeis J, Traugott M, Collatz J (2018) A simple and cost-effective molecular method to track predation on Drosophila suzukii in the field. J Pest Sci 91:927–935Google Scholar
  42. Woltz JM, Lee JC (2017) Pupation behavior and larval and pupal biocontrol of Drosophila suzukii in the field. Biol Control 110:62–69Google Scholar
  43. Woltz JM, Donahue KM, Bruck DJ, Lee JC (2015) Efficacy of commercially available predators, nematodes and fungal entomopathogens for augmentative control of Drosophila suzukii. J Appl Entomol 139:759–770Google Scholar

Copyright information

© International Organization for Biological Control (IOBC) 2019

Authors and Affiliations

  • Matthew T. Kamiyama
    • 1
  • Zachary Schreiner
    • 2
  • Christelle Guédot
    • 1
    Email author
  1. 1.Department of EntomologyUniversity of Wisconsin-MadisonMadisonUSA
  2. 2.Peninsular Agricultural Research Station, University of Wisconsin-MadisonSturgeon BayUSA

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