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Bio-herbicidal effect of 5-aminoleveulinic acid producing rhizobacteria in suppression of Lathyrus aphaca weed growth

  • Manisha PhourEmail author
  • Satyavir Singh Sindhu
Article
  • 18 Downloads

Abstract

The present study evaluated the herbicidal potential of rhizospheric bacteria against Lathyrus aphaca L. weed in Indian mustard (Brassica juncea L.) crop. In primary screening, bacterial isolates HMM76, HMM109, HMM116, JMM24 and JMM44 were found to produce aminolevulinic acid ranging from 10.0 to 15.0 µg ml−1 with HMM21, JMM11 and JMM35 producing more than 15 µg ml−1. In secondary screening, ten bacterial isolates i.e., HMM21, HMM57, HMM76, HMM83, HMM109, HMM116, JMM4, JMM24, JMM35 and JMM44 showed a growth retardation effect on the 5th and 10th day after seed germination. Moreover, evaluation of screened bacterial isolates exhibiting different plant growth promoting traits such as indole acetic acid (IAA) production, phosphorus and potassium solubilization, 1-aminocyclopropane-1-carboxylate deamainase enzyme, and antagonistic activity against potential pathogens were used to conduct pot studies and found to cause significant reduction up to 92% in root and shoot dry weight of L. aphaca. The best performing culture, JMM24, was identified as Bacillus flexus by 16S rRNA sequence analysis. Therefore, these rhizospheric bacterial isolates could act as a potential candidate for suppression of weed growth under field conditions for their subsequent application as bioherbicide.

Keywords

5-aminolevulinic acid Growth suppression Lathyrus aphaca Mustard Rhizospheric bacteria 

Notes

Acknowledgements

The work described here was supported by funds from the Indian Council of Agricultural Research, New Delhi, India and from the grant provided by CCS Haryana Agricultural University, Hisar.

Supplementary material

10526_2019_9925_MOESM1_ESM.doc (5.8 mb)
Supplementary material 1 (DOC 5892 kb)

References

  1. Abbas T, Zahir ZA, Naveed M (2017) Bioherbicidal activity of allelopathic bacteria against weeds associated with wheat and their effects on growth of wheat under axenic conditions. BioControl 62(5):719–730CrossRefGoogle Scholar
  2. Banga RS, Yadav A (2001) Evaluation of herbicides against complex weed flora in Indian mustard. Haryana J Agron 17:48–51Google Scholar
  3. Bhan VM (1992) Weed management—a factor for sustainability in crop production. In: Proceedings of XII National symposium on resource management for sustained crop production, Rajasthan Agriculture University, India, Bikaner pp 209–216Google Scholar
  4. Bouizgarne B, El-Maarouf-Bouteau H, Madiona K, BiliguiB Monestiez M, Pennarun A, Amiar Z, Rona J, Ouhdouch Y, El Hadrami I, Bouteau F (2006) A putative role for fusaric acid in biocontrol of the parasitic angiosperm Orobanche ramosa. Mol Plant Microbe Interact 19:550–556CrossRefGoogle Scholar
  5. Charudattan R, Dinoor A (2000) Biological control of weeds using plant pathogens: accomplishments and limitations. Crop Protect 19:691–695CrossRefGoogle Scholar
  6. Chon SU (2003) Herbicidal activity of δ-aminolevulinic acid on several plants as affected by application methods. Korean J Crop Sci 48:50–58Google Scholar
  7. Clark K, Karsch-Mizrachi I, Lipman DJ, Ostell J, Sayers EW (2015) GenBank. Nucleic Acids Res 44(D1):D67–72CrossRefGoogle Scholar
  8. DeLuna L, Stubbs T, Kennedy A, Kremer RJ (2005) Deleterious bacteria in the rhizosphere. In: Zobel R, Wright S (eds.) Roots and soil management: interactions between roots and the soil. Monograph No. 48. Madison, USA, pp 233–261Google Scholar
  9. Dworkin M, Foster J (1958) Experiments with some microorganisms which utilize ethane and hydrogen. J Bacteriol 75:592–601Google Scholar
  10. Fredrickson JK, Elliott LF (1985) Colonization of winter wheat roots by inhibitory rhizobacteria. Soil Sci Soc Am J 49:1172–1177CrossRefGoogle Scholar
  11. Gealy DR, Gurusiddah S Jr, Ogg AG, Kennedy AC (1996) Metabolites from Pseudomonas fluorescens strain D7 inhibit downy brome (Bromus tectorum) seedling growth. Weed Technol 10:282–287CrossRefGoogle Scholar
  12. Gill HS, Sandhu KS, Mehra SP, Singh T (1984) Efficacy of some herbicides for control of weeds in Indian mustard. Indian J Agron 16:171–175Google Scholar
  13. Gordan SA, Weber RP (1951) Colorimetric estimation of indole acetic acid. Plant Physiol 26:192–195CrossRefGoogle Scholar
  14. Gurusiddaiah S, Gealy D, Kennedy A Jr, Ogg A (1994) Isolation and characterization of metabolites from Pseudomonas fluorescens strain D7 for control of downy brome (Bromus tectorum L.). Weed Sci 42:492–501Google Scholar
  15. Hotta Y, Tanaka H, Takaoka Y, Takeuchi Y, Konnai M (1997) Promotive effects of 5-aminolevulinic acid on the yield of several crops. Plant Growth Regul 22:109–114CrossRefGoogle Scholar
  16. Hu XF, Chen J, Guo JF (2006) Two phosphate and potassium solubilizing bacteria isolated from Tiannu mountain, Zhejiang, China. World J Microbiol Biotechnol 22:983–990CrossRefGoogle Scholar
  17. Hyun KR, Song HG (2007) Effects of application of Rhodopseudomonas sp. on seed germination and growth of tomato under axenic conditions. J Microbiol Biotechnol 17:1805–1810Google Scholar
  18. Islam MA, Nain Z, Alam MK, Banu NA, Islam MR (2018) In vitro study of biocontrol potential of rhizospheric Pseudomonas aeruginosa against Fusarium oxysporum f. sp. cucumerinum. Egypt J Biol Pest Control 28(1):90–100CrossRefGoogle Scholar
  19. Jangu OP, Sindhu SS (2011) Differential response of inoculation with indole acetic acid producing Pseudomonas sp. in green gram (Vigna radiata L.) and black gram (Vigna mungo L.). Microbiol J 1:159–173CrossRefGoogle Scholar
  20. Johnson A, Booth C (1983) Plant pathologist’s pocket book, 2nd edn. Commonwealth Agricultural Bureaux, SurreyGoogle Scholar
  21. Kantha T, Kantachote D, Klongdee N (2015) Potential of biofertilizers from selected Rhodopseudomonas palustris strains to assist rice (Oryza sativa L. subsp. indica) growth under salt stress and to reduce greenhouse gas emissions. Ann Microbiol 65:2109–2118CrossRefGoogle Scholar
  22. Kennedy A, Stubbs T (2007) Management effects on the incidence of jointed goat grass inhibitory rhizobacteria. Biol Control 40:213–221CrossRefGoogle Scholar
  23. Kennedy AC, Elliott LF, Young FL, Douglas CL (1991) Rhizobacteria suppressive to the weed downy brome. Soil Sci Soc Am J 55:722–727CrossRefGoogle Scholar
  24. Kennedy AC, Johnson BN, Stubbs TL (2001) Host range of a deleterious rhizobacterium for biological control of downy brome. Weed Sci 49:792–797CrossRefGoogle Scholar
  25. Kim SJ, Kremer RJ (2005) Scanning and transmission electron microscopy of root colonization of morning glory (Ipomoea spp.) seedlings by rhizobacteria. Symbiosis 39:117–124Google Scholar
  26. Kremer RJ (1987) Bacteria can battle weed growth. Am Nurserym 164:162–163Google Scholar
  27. Kremer RJ, Kennedy AC (1996) Rhizobacteria as biocontrol agents of weeds. Weed Technol 10(3):601–609CrossRefGoogle Scholar
  28. Kremer R, Souissi T (2001) Cyanide production by rhizobacteria and potential for suppression of weed seedling growth. Curr Microbiol 43:182–186CrossRefGoogle Scholar
  29. Kremer RJ, Caesar A, Souissi T (2006) Soilborne microorganisms of Euphorbia are potential biological control agents of the invasive weed leafy spurge. Appl Soil Ecol 32:27–37CrossRefGoogle Scholar
  30. Kroschel J, Elzein A (2004) Bioherbicidal effect of fumonisin B1, a phytotoxic metabolite naturally produced by Fusarium nygamai, on parasitic weeds of the genus Striga. Biocontrol Sci Technol 14:117–128CrossRefGoogle Scholar
  31. Lakshmi V, Kumari S, Singh A, Prabha C (2015) Isolation and characterization of deleterious Pseudomonas aeruginosa KC1 from rhizospheric soils and its interaction with weed seedlings. J King Saud Univ Sci 27:113–119CrossRefGoogle Scholar
  32. Liu S, Zhang G, Li X, Zhang J (2014) Microbial production and applications of 5-aminolevulinic acid. Appl Microbiol Biotechnol 98:7349–7357CrossRefGoogle Scholar
  33. Loper JE, Schroth MN (1986) Influence of bacterial sources of indole-3-acetic acid on root elongation of sugarbeet. Phytopathology 76:386–389CrossRefGoogle Scholar
  34. Mauzerall D, Granick S (1955) The occurrence and determination of δ-aminolevulinic acid and porphobilinogen in urine. J Biol Chem 219:435–446Google Scholar
  35. Mazzola M, Stahlman PW, Leach JE (1995) Application method affects the distribution and efficacy of rhizobacteria suppressive of downy brome (Bromus tectorum). Soil Biol Biochem 27:1271–1278CrossRefGoogle Scholar
  36. Mohan Babu R, Sajeena A, Vidhyasekaran P, Seetharaman K, Reddy MS (2003) Characterization of a phytotoxic glycoprotein produced by Phoma eupyrena—a pathogen on water lettuce. Phytoparasitica 31:265–274CrossRefGoogle Scholar
  37. Norman MA, Patten KD, Gurusiddaiah S (1994) Evaluation of a phytotoxin(s) from Pseudomonas syringae for weed control in cranberries. Hortic Sci 29:1475–1477Google Scholar
  38. Olsen J, Kristensen L, Weiner J (2005) Effects of density and spatial pattern of winter wheat on suppression of different weed species. Weed Sci 53:690–694CrossRefGoogle Scholar
  39. Owen A, Zdor R (2001) Effect of cyanogenic rhizobacteria on the growth of velvetleaf (Abutilon theophrasti) and corn (Zea mays) in autoclaved soil and the influence of supplemental glycine. Soil Biol Biochem 33:801–809CrossRefGoogle Scholar
  40. Pandey KK, Mayilraj S, Chakrabarti T (2002) Pseudomonas indica sp. nov., a novel butane-utilizing species. Int J Syst Evol Microbiol 52:1559–1567Google Scholar
  41. Patil VS (2014) Isolation, characterization and identification of rhizospheric bacteria with the potential for biological control of Sida acuta. J Environ Res Dev 8:411–417Google Scholar
  42. Patten CL, Glick BR (1996) Bacterial biosynthesis of indole-3-acetic acid. Can J Microbiol 42(3):207–220CrossRefGoogle Scholar
  43. Pattern CL, Glick BR (2002) Role of Pseudomoms putida indole acetic acid in development of the host plant root system. Appl Environ Microbiol 68:3795–3801CrossRefGoogle Scholar
  44. Penrose DM, Glick BR (2003) Methods for isolating and characterizing ACC deaminase containing plant growth-promoting rhizobacteria. Physiol Plant 118:10–15CrossRefGoogle Scholar
  45. Phour M, Ghai A, Rose G, Dhull N, Sindhu SS (2018) Role of aminolevulinic acid in stress adaptation and crop productivity. Int J Curr Microbiol App Sci 7(05):1516–1524CrossRefGoogle Scholar
  46. Pitcher DG, Saunders NA, Owen RJ (1989) Rapid extraction of bacterial genomic DNA with guanidium thiocyanate. Lett Appl Microbiol 8:151–156CrossRefGoogle Scholar
  47. Radhakrishnan R, Park J, Lee IJ, Abd_Allah EF, Hashem A (2017) Bio-herbicide effect of salt marsh tolerant Enterobacter sp. i-3 on weed seed germination and seedling growth. Pak J Bot 49(5):1959–1963Google Scholar
  48. Saikeur A, Choorit W, Prasertsan P, Kantachote D, Sasaki K (2009) Influence of precursors and inhibitor on the production of extracellular 5-aminolevulinic acid and biomass by Rhodopseudomonas palustris kG31. Biosci Biotechnol Biochem 73:987–992CrossRefGoogle Scholar
  49. Samad A, Antonielli L, Sessitsch A, Compant S, Trognitz F (2017) Comparative genome analysis of the vineyard weed endophyte Pseudomonas viridiflava CDRTc14 showing selective herbicidal activity. Sci Rep 7(1):17336–17350CrossRefGoogle Scholar
  50. Saraf M, Pandya U, Thakkar A (2014) Role of allelochemicals in plant growth promoting rhizobacteria for biocontrol of phytopathogens. Microbiol Res 169:18–29CrossRefGoogle Scholar
  51. Sasikala Ch, Ramana ChV, Rao PR (1994) 5-aminolevulinic acid: a potential herbicide/insecticide from microorganisms. Biotechnol Prog 10:451–459CrossRefGoogle Scholar
  52. Sharma R, Sindhu S, Sindhu SS (2018) Suppression of Alternaria blight disease and plant growth promotion of mustard (Brassica juncea L.) by antagonistic rhizosphere bacteria. Appl Soil Ecol 129:145–150CrossRefGoogle Scholar
  53. Siddiqui MA, Chauhan PS, Anandham R, Han GH, Tongmin S (2010) Isolation, characterization, and use for plant growth promotion under salt stress, of acc deaminase-producing halotolerant bacteria derived from coastal soil. J Microbiol Biotechnol 20:1577–1584CrossRefGoogle Scholar
  54. Sindhu SS, Gupta SK, Dadarwal KR (1999) Antagonistic effect of Pseudomonas spp. on pathogenic fungi and enhancement of growth of green gram (Vigna radiata). Biol Fertil Soils 29:62–68CrossRefGoogle Scholar
  55. Singh SS (1992) Effect of fertilizer application and weed control on the yield of mustard (Brassica juncea). Indian J Agron 37:196–198Google Scholar
  56. Tranel PJ, Gealy DR, Kennedy AC (1993) Inhibition of downy brome (Bromus tectorum) root growth by a phytotoxin from Pseudomonas fluorescens strain D7. Weed Technol 7:134–139CrossRefGoogle Scholar
  57. Vessey JK (2003) Plant growth promoting rhizobacteria as biofertilizers. Plant Soil 255:571–586CrossRefGoogle Scholar
  58. Zermane N, Souissi T, Kroschel J, Sikora R (2007) Biocontrol of broom rape (Orobanche crenata Forsk. and Orobanche foetida Poir.) by Pseudomonas fluorescens isolate Bf7-9 from the faba bean rhizosphere. Biocontrol Sci Technol 17:487–497CrossRefGoogle Scholar

Copyright information

© International Organization for Biological Control (IOBC) 2019

Authors and Affiliations

  1. 1.Department of Microbiology, College of Basic Sciences and HumanitiesCCS Haryana Agricultural UniversityHisarIndia

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