The role of DNA repair genes in radiation-induced adaptive response in Drosophila melanogaster is differential and conditional

  • Liubov Koval
  • Ekaterina Proshkina
  • Mikhail Shaposhnikov
  • Alexey MoskalevEmail author
Research Article


Studies in human and mammalian cell cultures have shown that induction of DNA repair mechanisms is required for the formation of stimulation effects of low doses of ionizing radiation, named “hormesis”. Nevertheless, the role of cellular defense mechanisms in the formation of radiation-induced hormesis at the level of whole organism remains poorly studied. The aim of this work was to investigate the role of genes involved in different mechanisms and stages of DNA repair in radioadaptive response and radiation hormesis by lifespan parameters in Drosophila melanogaster. We studied genes that control DNA damage sensing (D-Gadd45, Hus1, mnk), nucleotide excision repair (mei-9, mus210, Mus209), base excision repair (Rrp1), DNA double-stranded break repair by homologous recombination (Brca2, spn-B, okr) and non-homologous end joining (Ku80, WRNexo), and the Mus309 gene that participates in several mechanisms of DNA repair. The obtained results demonstrate that in flies with mutations in studied genes radioadaptive response and radiation hormesis are absent or appear to a lesser extent than in wild-type Canton-S flies. Chronic exposure of γ-radiation in a low dose during pre-imaginal stages of development leads to an increase in expression of the studied DNA repair genes, which is maintained throughout the lifespan of flies. However, the activation of conditional ubiquitous overexpression of DNA repair genes does not induce resistance to an acute exposure to γ-radiation and reinforces its negative impact.


Drosophila melanogaster Lifespan DNA repair genes Radioadaptive response Radiation hormesis Ionizing radiation 



We are grateful to Dr. Schupbach (Princeton University, USA), Dr. Abdu (Ben-Gurion University, Israel), Bloomington (Indiana University, USA) and Kyoto (Kyoto Institute of Technology, Japan) Stock Centers for providing the Drosophila strains. We thank the Genetivision (Houston, USA) for transgenic fly services, to the Drosophila Genomics Resource Center (Indiana University, USA) for cDNA clones.


The study was carried out within the framework of the state task on themes “Molecular-genetic mechanisms of aging, lifespan, and stress resistance of Drosophila melanogaster”, state registration № AAAA-A18-118011120004-5, “Development of geroprotective and radioprotective agents”, state registration № AAAA-A19-119021590022-2 and complex UrB RAS Programme № 18-7-4-23 “A combination of factors of different nature (low temperature, lack of lighting, restrictive diet, and geroprotector) to maximize the lifespan of Drosophila”, state registration № AAAA-A18-118011120008-3.

Compliance with ethical standards

Conflict of interest

The authors have no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.

Supplementary material

10522_2019_9842_MOESM1_ESM.pdf (336 kb)
Supplementary material 1 (PDF 336 kb)


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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.Laboratory of Geroprotective and Radioprotective TechnologiesInstitute of Biology, Komi Science Center, Ural Branch, Russian Academy of SciencesSyktyvkarRussian Federation
  2. 2.Pitirim Sorokin Syktyvkar State UniversitySyktyvkarRussian Federation
  3. 3.Laboratory of Post-Genomic ResearchEngelhardt Institute of Molecular Biology, Russian Academy of SciencesMoscowRussian Federation
  4. 4.Moscow Institute of Physics and TechnologyDolgoprudnyRussian Federation

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