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Age-related changes in mitochondrial membrane composition of Nothobranchius furzeri.: comparison with a longer-living Nothobranchius species

  • Pedro F. Almaida-Pagan
  • Carmen Ortega-Sabater
  • Alejandro Lucas-Sanchez
  • Daniel Gonzalez-Silvera
  • Antonio Martinez-Nicolas
  • Maria Angeles Rol de Lama
  • Pilar Mendiola
  • Jorge de Costa
Research Article

Abstract

Membrane compositions, particularly of mitochondria, could be critical factors in the mechanisms of growth and aging, especially during phases of high oxidative stress that result in molecular damage. Changes affecting lipid class or fatty acid (FA) compositions could affect phospholipid (PL) properties and alter mitochondrial function. In the present study, mitochondrial membrane PL compositions were analysed throughout the life-cycle of Nothobranchius furzeri, a species with explosive growth and one of the shortest-lived vertebrates. Mitochondrial PLs showed several changes with age. Proportions of total PLs and PC were reduced while an increase in PS, CL and PE was observed, mainly between the 2.5 and 5 months of fish age, the time during which animals doubled their weight. FA compositions of individual PLs in mitochondria were also significantly affected with age suggesting the existence of increasing damage to mitochondrial lipids during the life-cycle of N. furzeri that could be one of the main contributors to degraded mitochondrial function associated with aging. The peroxidation index values from N. furzeri mitochondrial PLs were significantly lower than those reported in N. rachovii, a species with a twofold longer life span than N. furzeri, which seems to contradict the membrane pacemaker theory of animal metabolism.

Keywords

Biology of aging Mitochondria Fish Lipids 

Notes

Acknowledgements

Pedro F. Almaida-Pagan was funded by a research grant from the Fundación ONCE, as part of the “Oportunidad al Talento” Programme for disabled scientists (co-financed by ESF). This work was supported by the Ministry of Economy and Competitiveness, the Instituto de Salud Carlos III through CIBERFES (CB16/10/00239) and Grant 19899/GERM/15 (co-financed by FEDER).

Supplementary material

10522_2018_9778_MOESM1_ESM.docx (28 kb)
Supplementary material 1 (DOCX 28 kb)

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Copyright information

© Springer Nature B.V. 2018

Authors and Affiliations

  • Pedro F. Almaida-Pagan
    • 1
    • 2
    • 4
  • Carmen Ortega-Sabater
    • 1
    • 2
  • Alejandro Lucas-Sanchez
    • 1
    • 2
  • Daniel Gonzalez-Silvera
    • 3
  • Antonio Martinez-Nicolas
    • 1
    • 2
  • Maria Angeles Rol de Lama
    • 1
    • 2
  • Pilar Mendiola
    • 1
    • 2
  • Jorge de Costa
    • 1
    • 2
  1. 1.Chronobiology Lab, Department of Physiology, College of BiologyUniversity of MurciaMurciaSpain
  2. 2.Ciber Fragilidad y Envejecimiento Saludable (CIBERFES)MadridSpain
  3. 3.Department of Cell Biology, College of BiologyUniversity of MurciaMurciaSpain
  4. 4.Department of Physiology, Faculty of BiologyUniversity of MurciaMurciaSpain

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