The secretory phenotype of senescent astrocytes isolated from Wistar newborn rats changes with anti-inflammatory drugs, but does not have a short-term effect on neuronal mitochondrial potential
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In the central nervous system (CNS), senescent astrocytes have been associated with neurodegeneration. Senescent cells secrete a complex mixture of pro-inflammatory factors, which are collectively called Senescence Associated Secretory Phenotype (SASP). The SASP components can vary depending on the cell type, senescence inducer and time. The SASP has been mainly studied in fibroblasts and epithelial cells, but little is known in the context of the CNS. Here, the SASP profile in senescent astrocytes isolated from Wistar newborn rats induced to senescence by oxidative stress or by proteasome inhibition was analyzed. Senescent astrocytes secreted predominantly chemokines and IL-1α, but no IL-6. The effect of the anti-inflammatory drugs, sulforaphane (SFN) and dehydroepiandrosterone (DHEA), on the SASP profile was evaluated. Our results showed that SFN and DHEA decreased IL-1α secretion while increasing IL-10, thus modifying the SASP to a less anti-inflammatory profile. Primary neurons were subjected to the conditioned media obtained from drug-treated senescent astrocytes, and their mitochondrial membrane potential was evaluated.
KeywordsSenescence inflammation central nervous system Astrocytes Neurons
The authors would like to thank Dr. Rocío González-Vieira from UAM-I for animal supply and Dr. Ruth Rincón Heredia from IFC-UNAM and the CBS-UAMI Confocal Core for confocal images acquisition and analysis. Dr. Anahí Chavarría for her guidance in the cytokines field. This work was supported by CONACyT grant FON.INST/298/2016, as well as the “Red Temática de Investigación en Salud y Desarrollo Social” from CONACYT. Maciel-Barón, Silva-Palacios and Morales-Rosales are CONACyT scholarship holders.
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Conflict of interest
The authors have no conflicts of interest to disclose.
- Coppe JP, Rodier F, Patil CK, Freund A, Desprez PY, Campisi J (2011) Tumor suppressor and aging biomarker p16(INK4a) induces cellular senescence without the associated inflammatory secretory phenotype. J Biol Chem 286:36396–36403. https://doi.org/10.1074/jbc.M111.257071 CrossRefPubMedPubMedCentralGoogle Scholar
- Hubackova S, Krejcikova K, Bartek J, Hodny Z (2012) IL1- and TGFbeta-Nox4 signaling, oxidative stress and DNA damage response are shared features of replicative, oncogene-induced, and drug-induced paracrine ‘bystander senescence’. Aging (Albany NY) 4:932–951. https://doi.org/10.18632/aging.100520 CrossRefGoogle Scholar
- Negrette-Guzman M, Huerta-Yepez S, Tapia E, Pedraza-Chaverri J (2013) Modulation of mitochondrial functions by the indirect antioxidant sulforaphane: a seemingly contradictory dual role and an integrative hypothesis. Free Radic Biol Med 65:1078–1089. https://doi.org/10.1016/j.freeradbiomed.2013.08.182 CrossRefPubMedGoogle Scholar