Advertisement

Method for Determining Microcirculatory Hemodynamics by Laser Speckle Interferometry in Small Animals

We performed experimental evaluation of various positions (ear, paw, and tail) of the of dynamic light scattering sensor of near-infrared laser radiation (840 nm) using speckle interferometry in rats. Dynamic monitoring of the reactions to prolonged ultrasound exposure (7 days) showed that the base of the tail is most suitable for this purpose. Additionally, analysis of microcirculation parameters on the surface of skin autograft was performed. The method of analysis of hemodynamics in humans was adapted for animals.

This is a preview of subscription content, log in to check access.

Access options

Buy single article

Instant unlimited access to the full article PDF.

US$ 39.95

Price includes VAT for USA

Subscribe to journal

Immediate online access to all issues from 2019. Subscription will auto renew annually.

US$ 199

This is the net price. Taxes to be calculated in checkout.

References

  1. 1.

    Aleksandrin VV. Wavelet analysis of cerebral blood flow in rats. Regional blood circulation and microcirculation. Regionar. Krovoobr. Mikrotsirk. 2010;9(4):63-66. Russian.

  2. 2.

    Belichenko VМ, Grigoreva ТA, Shoshenko СA. The muscular blood flow in rats in ontogenesis as measured by the needle probe laser doppler flowmeter “LAKK-01”. Ross. Fiziol. Zh. 2007;93(6):655-660. Russian.

  3. 3.

    Blazhko AA, Shakhmatov II, Kiselev VI, Lycheva NA, Moskalenko SV. Changes of microcirculation in rats detected by laser Doppler flowmetry at physical load accompanied by the development of thrombotic readiness. Regionar. Krovoobr. Mikrotsirk. 2017;16(4):60-64. Russian.

  4. 4.

    Ladnich NA, Smolyakov YuN. Possible options for the formation of integrated indicators for assessing biomedical research. Informatika Sistemy Upravleniya. 2007;(S1):16-18. Russian.

  5. 5.

    Fine I, Kaminsky A. Speckle-based measurement of the light scattering by red blood cells in vivo. Dynamics and Fluctuations in Biomedical Photonics VIII. International Society for Optics and Photonics, 2011;7898. ID 78980A. doi: https://doi.org/10.1117/12.881991

  6. 6.

    Fine I, Kaminsky AV, Shenkman L. A new sensor for stress measurement based on blood flow fluctuations. Dynamics and Fluctuations in Biomedical Photonics XII. Tuchin VV, ed. SPIE Press, 2016;9707. ID 970705. doi: https://doi.org/10.1117/12.2212866

  7. 7.

    Handbook of Photonics for Biomedical Science. Tuchin VV, ed. CRC Press, 2010.

  8. 8.

    Kuznik BI, Smolyakov YN, Davydov SO, Tsybikov NN, Maksimova OG, Malinina AV, Shenkman L, Kaminsky A, Fine I. Impact of fitness status on the optically measured hemodynamic indexes. J. Healthc. Eng. 2018;2018. ID 1674931. doi: 10.1155/2018/1674931

  9. 9.

    Nemeth N, Lesznyak T, Brath E, Acs G, Nagy A, Pap-Szekeres J, Furka I, Miko I. Changes in microcirculation after ischemic process in rat skeletal muscle. Microsurgery. 2003;23(5):419-423.

  10. 10.

    R Core Team (2018). R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. URL: https://www.R-project.org; Available at: https://www.R-project.org (Accessed 23 January 2019).

  11. 11.

    Ul’Yanov SS, Tuchin VV, Bednov AA, Brill GE, Zakharova EI. The application of speckle interferometry for the monitoring of blood and lymph flow in microvessels. Lasers Med. Sci. 1997;12(1):31-41.

Download references

Author information

Correspondence to Y. N. Smolyakov.

Additional information

Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 168, No. 9, pp. 387-390, September, 2019

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Smolyakov, Y.N., Kuznik, B.I., Bondarchuk, J.A. et al. Method for Determining Microcirculatory Hemodynamics by Laser Speckle Interferometry in Small Animals. Bull Exp Biol Med 168, 410–412 (2020) doi:10.1007/s10517-020-04720-0

Download citation

Key Words

  • speckle
  • microcirculation
  • small animals