Bulletin of Experimental Biology and Medicine

, Volume 168, Issue 1, pp 38–40 | Cite as

Permeability of Intestinal and Blood—Tissue Barriers in Rats for Evans Blue Dye under Conditions of Acute Intoxication with Cyclophosphamide

  • T. V. SchäferEmail author
  • Ju. Ju. Ivnitsky
  • V. L. Rejniuk

Myeloablative therapy was modeled by administration of cyclophosphamide to rats in a dose of 2.1 LD50 for 14 days. Under these conditions, increased accumulation of Evans blue dye given by gavage was observed in the blood, brain, lung, liver, kidney, and ileum (by 34, 1.6, 149, 46, 30, and 6.2 times, respectively). After intravenous injection of the dye, its accumulation was increased only in the lung and ileum. Thus, simulation of myeloablation cytostatic therapy by cyclophosphamide injection to rats is associated with impairment of the intestinal and lung—blood barriers. These alterations predispose to penetration of biologically active substances from the small intestine, gastrointestinal chyme, and lungs to the blood, thus contributing to the development of the early toxic effects of cyclophosphamide.

Key Words

cyclophosphamide acute intoxication Evans blue intestinal barrier tissueblood barrier 


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • T. V. Schäfer
    • 1
    Email author
  • Ju. Ju. Ivnitsky
    • 2
  • V. L. Rejniuk
    • 2
  1. 1.State Research Test Institute of Military MedicineMinistry of Defense the Russian FederationSt. PetersburgRussia
  2. 2.Institute of ToxicologyFederal Medical- Biological Agency of RussiaSt. PetersburgRussia

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