Comparative Analysis of JNK1 Expression in Liver Cells in Rats of Different Lines Receiving Excess of Easily Digested Carbohydrates: Confocal Microscopy
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The expression of JNK1 isoform of cJun-N-terminal kinase in hepatocytes of rats receiving excess of simple carbohydrates dissolved in drinking water was studied by immunohisto-chemical staining and confocal microscopy. In experiment I (60 days), the highest expression of JNK1 was observed in female Wistar rats receiving fructose (the difference with the group receiving a mixture of glucose and fructose was significant, p<0.05, the difference with the control group at the trend level, p=0.077; Mann—Whitney U test). In experiment II (120 days), an increase in JNK1 expression was observed in Wistar rats (males and females) receiving 30% fructose. In Dark Aguti rats (females and males) of comparable age, increased basal level of JNK1 expression was observed in comparison with Wistar rats. Three-way ANOVA showed that JNK1 expression was significantly (p<0.05) associated with consumption of fructose and animal line, but not sex. The level of JNK1 expression corresponded to previously identified changes in the biochemical markers of the metabolic syndrome.
Key WordsJNK1 rats expression fructose metabolic syndrome
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- 1.Apryatin SA, Mzhelskaya KV, Balakina AS, Soto SJ, Beketova NA, Kosheleva OV, Kodentsova VM, Gmoshinsky IV. Sex and line differences in biochemical indices and fat-soluble vitamins sufficiency in rats on in vivo model of metabolic syndrome. Vopr. Pitaniya. 2018;87(1):51-62. Russian.Google Scholar
- 2.Apryatin SA, Mzhel’skaya KV, Trusov NV, Balakina AS, Soto CJ, Vrzhesinskaya OA, Guseva GV, Aksenov IV, Beketova NA, Kosheleva OV, Kodentsova VM, Gmoshinsky IV. Comparative evaluation of vitamin status and biochemical markers of metabolic syndrome on the models of rodents receiving rations with high quotas of different sugars. Vopr. Pitaniya. 2017;86(6):42-55. Russian.Google Scholar
- 3.Metabolic Syndrome. Roitberg GE, ed. Moscow, 2007. Russian.Google Scholar
- 13.Wong SK, Chin KY, Suhaimi FH, Fairus A, Ima-Nirwana S. Animal models of metabolic syndrome: a review. Nutr. Metab. (Lond). 2016;13:65.Google Scholar