Bulletin of Experimental Biology and Medicine

, Volume 167, Issue 5, pp 653–655 | Cite as

In Vitro Analysis of the Expression of CD11, CD29, CD36, and DC-STAMP Molecules during the Formation of Multinuclear Macrophages in BCG-Infected Mice

  • D. A. Il’inEmail author
  • V. A. Shkurupy

Expression of CD11, CD29, CD36, and DC-STAMP molecules by macrophages was analyzed in in vitro experiments. These molecules mediate cell fusion, one of the mechanisms underlying the formation of multinuclear macrophages. Macrophages were obtained from intact and BCG-infected male BALB/c mice. In intact cultures, multinuclear macrophages appeared primarily due to amitotic division of cell nuclei, while in macrophage cultures from infected mice, the process of cell fusion predominated. In intact macrophage cultures, bi- and multinuclear cells expressed primarily CD29 and CD36. In cultures from infected mice, macrophages expressing CD29 and DC-STAMP predominated, but bi- and multinuclear macrophages expressing CD11 and CD36 predominated over mononuclear ones. The study of macrophage fusion mechanism can be useful for understanding of this biological phenomenon as the mechanisms of delivery of M. tuberculosis and lysosomotropic anti-tuberculosis drugs into tuberculous granulomas to suppress M. tuberculosis persisting in macrophages and reduce the destructive potential of granulomas.

Key Words

macrophages cell fusion cell adhesion molecules Mycobacterium tuberculosis 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Iljine DA, Arkhipov SA, Shkurupy VA. In vitro expression of IL-1α, GM-CSF, and TNF-α by multinucleated macrophages from BCG-infected mice. Bull. Exp. Biol. Med. 2013; 155(5):663-666.CrossRefGoogle Scholar
  2. 2.
    Il’in DA, Arkhipov SA, Shkurupy VA. In Vitro Study of Cytophysiological Characteristics of Multinuclear Macrophages from Intact and BCG-Infected Mice. Bull. Exp. Biol. Med. 2016;160(5):668-671.CrossRefGoogle Scholar
  3. 3.
    Il’in DA, Arkhipov SA, Shkurupy VA. Analysis of IL-1α, bFGF, TGF-β1, IFNγ, MMP-1, and CatD Expression in Multinuclea Macrophages In Vitro. Bull. Exp. Biol. Med. 2018; 164(4):456-458.CrossRefGoogle Scholar
  4. 4.
    Il’in DA, Shkurupy VA. Expression of Pro- and Antiapoptotic Factors in Multinuclear Macrophages of BCG-Infected Mice In Vitro. Bull. Exp. Biol. Med. 2018;165(4):482-485.CrossRefGoogle Scholar
  5. 5.
    Shkurupy VA. Tuberculous Granulomatosis. Cytophysiology and Target Treatment. Moscow, 2007. Russian.Google Scholar
  6. 6.
    Shkurupy VA, Ilin DA, Arkhipov SA. Polynuclearity phenomenon - macrophage response to M. tuberculosis persistence in macrophages. Mezhdunarod. Zh. Priklad. Fundament. Issled. 2017;(11-2):218-222. Russian.Google Scholar
  7. 7.
    Shkurupiy VA, Kim LB, Nikonova IK, Potapova OV, Cherdantseva LA, Sharkova TV. Hydroxyproline content and fibrogenesis in mouse liver and lungs during the early stages of BCG granulomatosis. Bull. Exp. Biol. Med. 2013;154(3):299-302.CrossRefGoogle Scholar
  8. 8.
    Aziz MH, Cui K, Das M, Brown KE, Ardell CL, Febbraio M, Pluskota E, Han J, Wu H, Ballantyne CM, Smith JD, Cathcart MK, Yakubenko VP. The upregulation of integrin αDβ2 (CD11d/CD18) on inflammatory macrophages promotes macrophage retention in vascular lesions and development of atherosclerosis. J. Immunol. 2017;198(12):4855-4867.CrossRefGoogle Scholar
  9. 9.
    Helming L, Winter J, Gordon S. The scavenger receptor CD36 plays a role in cytokine-induced macrophage fusion. J. Cell Sci. 2009;122(Pt 4):453-459.CrossRefGoogle Scholar
  10. 10.
    Kim MY, Cho JY. Molecular association of CD98, CD29, and CD147 critically mediates monocytic U937 cell adhesion. Korean J. Physiol. Pharmacol. 2016;20(5):515-523.CrossRefGoogle Scholar
  11. 11.
    McNally AK, Anderson JM. Beta1 and beta2 integrins mediate adhesion during macrophage fusion and multinucleated foreign body giant cell formation. Am. J. Pathol. 2002;160(2):621-630.CrossRefGoogle Scholar
  12. 12.
    Yagi M, Miyamoto T, Sawatani Y, Iwamoto K, Hosogane N, Fujita N, Morita K, Ninomiya K, Suzuki T, Miyamoto K, Oike Y, Takeya M, Toyama Y, Suda T. DC-STAMP is essential for cell-cell fusion in osteoclasts and foreign body giant cells. J. Exp. Med. 2005;202(3):345-351.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Research Institute of Experimental and Clinical MedicineFederal Research Center of Fundamental and Translational MedicineNovosibirskRussia
  2. 2.Novosibirsk State Medical University, Ministry of Health of the Russian FederationNovosibirskRussia

Personalised recommendations