Advertisement

Structural and Functional Parameters of the Thymus in Mice Exposed to γ-Irradiation after Restraint Stress

  • L. M. ErofeevaEmail author
Article
  • 3 Downloads

In mice exposed to γ-irradiation in a dose of 2 Gy after 15-days restraint stress, the body weight decreased by 21% and thymus weight decreased by 33.3% in comparison with the control, and significant changes in the histological structure of the thymus were observed. The medullary substance prevailed over the cortical substance. The absolute number of cells per 1 mm2 of histological section was reduced in the subcapsular area and medullary substance. The analysis of cell composition in functional areas of the thymus showed the most pronounced changes in the cortical substance. The decrease in the number of proliferating cells and low-differentiated lymphocytes and the increase in the number of destructed cells reflected impairment of the lymphocytopoietic function of the thymus. A minor decrease in the number of small lymphocytes indicated impaired migration processes in the thymus of mice exposed to γ-irradiation after restraint stress. The observed complex of histological and physiological changes in the thymus can lead to dysfunction of the lymphatic (immune) system.

Key Words

thymus irradiation restraint stress hypokinesia space flight factors 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Antropova EN, Rykova MP, Meshkov DO, Kazantseva VA. Immunologic reactivity and state of the intestinal viral flora of humans in pressurized chambers. Aviakosm. Ekol. Med. 2000;34(5):24-29. Russian.Google Scholar
  2. 2.
    Berendeeva TA, Rykova MP, Antropova EN, Larina IM, Morukov BV. Human immunity system status during 7-day dry immersion. Aviakosm. Ekol. Med. 2009;43(5):36-42. Russian.Google Scholar
  3. 3.
    Grigoriev AI, Egorov AD, Potapov AN. Some medical problems of a piloted mission to Mars. Aviakosm. Ekol. Med. 2000;34(3):6-12. Russian.Google Scholar
  4. 4.
    Kalandarova MP, Rodina GP, Serova LV. Peculiarities of physiological and reparative regeneration of the bone marrow in rats exposed on biosatellites Cosmos-605 and 690. Probl. Gematol. Pereliv. Krovi. 1981;26(12):26-30. Russian.Google Scholar
  5. 5.
    Lesniak AT, Antropova EN, Meshkov DO, Rykova MP. Diagnostics of impairment of the T-cell component in the immune system during space flight. Aviakosm. Ekol. Med. 1999;33(3):62-67. Russian.Google Scholar
  6. 6.
    Morukov BV, Rykova MP, Antropova EN, Berendeeva TA, Ponomaryov SA, Larina IM. Parameters of the innate and adaptive immunity in cosmonauts after long-term space flight on board the international space station. Human Physiol. 2010;36(3):264-273.CrossRefGoogle Scholar
  7. 7.
    Morukov BV, Rykova MP, Antropova EN, Berendeeva TA, Ponomarev SA. The human system of immunity under the conditions of 105-day isolation and confinement in artificial environment. Human Physiol. 2014;40(7):814-821.CrossRefGoogle Scholar
  8. 8.
    Rykova MP. Immune system of Russian cosmonauts after orbital space flights. Human Physiol. 2013;39(5):557-566.CrossRefGoogle Scholar
  9. 9.
    Sapin MP, Erofeeva LM, Grigorenko DE, Fedorenko BS. Reaction of different functional zones in mouse thymus and spleen lymphoid tissue to γ-irradiation. Bull. Exp. Biol. Med. 1998;125(4):416-419.CrossRefGoogle Scholar
  10. 10.
    Sycheva LP, Shchegoleva RA, Lisina NI, Gordeev AV, Rozhdestvenskii LM. The Dependence of the Mutagenic Effect on the Dose of X-Ray Irradiation in an In Vivo Experiment on Female (CBA×C57Bl/6)F1 Mice. Bull. Exp. Biol. Med. 2018;166(1):43-45.CrossRefGoogle Scholar
  11. 11.
    Bertho JM, Gourmelon P. Human thymic stromal cell irradiation reduces intra-thymic T cell precursor proliferation: evidence for a soluble mediator. Int. J. Radiat. Biol. 1998;74(3):387-396.CrossRefGoogle Scholar
  12. 12.
    Mallya SM, Sikpi MO. Regoirement for p53 in ionizing-radiation-inhibition of double-strand-break rejoining by human lymphoblasts. Mutat. Res. 1999;434(2):119-132.CrossRefGoogle Scholar
  13. 13.
    Morukov B, Rykova M, Antropova E, Berendeeva T, Ponomaryov S, Larina I. T-cell immunity and cytokine production in cosmonauts after long-duration space flights. Acta Astronautica. 2011;68(7-8):739-746.CrossRefGoogle Scholar
  14. 14.
    Potter M, Bernstein A, Lee JM. The wst gene regulates multiple forms of thymocyte apoptosis. Cell. Immunol. 1998;188(2):111-117.CrossRefGoogle Scholar
  15. 15.
    Shephard RJ, Castellani JW, Shek PN. Immune deficits induced by strenuous exertion under adverseenvironmental conditions: manifestations and countermeasures. Crit. Rev. Immunol. 1998;18(6):545-568.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Research Institute of Human MorphologyMoscowRussia

Personalised recommendations