Development of a Bacteriophage Complex with Superhydrophilic and Superhydrophobic Nanotextured Surfaces of Metals Preventing Healthcare-Associated Infections (HAI)
We studied antibacterial properties of organo-inorganic hybrid coatings on the AMg2 aluminum alloy including superhydrophilic and superhydrophobic nanotextured metal substrates with applied bacteriophage particles. Bactericidal activity of surfaces after artificial contamination with a bacterial suspension was evaluated. To increase bactericidal effect of the plates, bacteriophage was sorbed on their surface. In the experiments simulating possible spreading of HAI pathogens, higher bactericidal activity of superhydrophilic surfaces in comparison with superhydrophobic ones. Application of bacteriophage particles did not prevent primary colonization of textured metal surfaces by strains used in the experiment, but in some cases increases their bactericidal activity.
Key Wordsbacteriophages nanoparticles aluminum healthcare-associated infections (HAI) bactericidal activity
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- 1.Akimkin VG. Groups of nosocomial infections and a systemic approach to their prevention at multidisciplinary hospital. Epidemiol. Infekts. Bol. 2003;(5):15-19. Russian.Google Scholar
- 2.Aleshkin AV, Zul’karneev ER, Larina YuV, Rubalsky OV, Kiseleva IA, Rubalskii EO, Efimova OG, Afanasiev SS, Bochkareva SS, Smirnova KN, Teplyi AD. Bio-decontamination and extending shelf-life of meat and fish pre-processed foods with bacteriophages. Astrakhan. Med. Zh. 2015;10(4):40-48. Russian.Google Scholar
- 3.Brusina EB, Rychagov IP. Epidemiology of Nosocomial septic Infections in Surgery. Novosibirsk, 2006. Russian.Google Scholar
- 4.D’yachkova SYa. Bactericidal properties in examinations blanket serum bactericide, lysozyme bactericidal properties in examinations blanket serum bactericide, lysozyme, β-lysines and sensitivity chemistry preparation to microorganisms. Vestn. Voronezh. Gos. Univer. Ser.: Khimiya, Biologiya, Farmatsiya. 2003;(1):96-98. Russian.Google Scholar
- 5.Onishchenko GG. Incidence of nosocomial infections in the Russian Federation. Gig. San. 2008;(3):1-6. Russian.Google Scholar
- 6.Boinovich LB, Modin EB, Aleshkin AV, Emelyanenko KA, Zulkarneev ER, Kiseleva IA, Vasiliev AL, Emelyanenko AM. Effective antibacterial nanotextured surfaces based on extreme wettability and bacteriophage seeding. ACS Appl. Nano Materials. 2018;1(3):1348-1359. doi: https://doi.org/10.1021/acsanm.8b00090.CrossRefGoogle Scholar
- 7.Bruzaud J, Tarrade J, Celia E, Darmanin T, Taffin de Givenchy E, Guittard F, Herry JM, Guilbaud M, Bellon-Fontaine MN. The design of superhydrophobic stainless steel surfaces by controlling nanostructures: a key parameter to reduce the implantation of pathogenic bacteria. Mater. Sci. Eng. C Mater. Biol. Appl. 2017;73:40-47.CrossRefGoogle Scholar
- 10.Singh M, Singh S, Prasad S, Gambhir I. Nanotechnology in medicine and antibacterial effect of silver nanoparticles. Dig. J. Nanomater. Biostruct. 2008;3(3):115-122.Google Scholar