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Effect of Glycosaminoglycans on Pathogenic Properties Far-Eastern Tick-Borne Encephalitis Virus

  • G. N. LeonovaEmail author
  • S. I. Belikov
Article

We studied the effect of sulfated glycosaminoglycan on the infection properties of high-virulence Dal’negorsk strain and low-virulence Primorye-437 of tick-borne encephalitis virus. Differences in reproductive activity of these strains and their tropism to the target cells were revealed. Glycosaminoglycan reduced pathogenetic activity of high-virulence strain in vitro, but had no effect on low-virulence strain. The interaction of imperfect virus particles of non-pathogen strain with the glycosaminoglycan led to their accumulation in cell, but in the culture medium of SPEV cells infected with experimental and control samples, accumulation of virus particles did not differ. The results on activity of glycosaminoglycan binding with strains differing by their biological and molecular genetic characteristics can be used to assess their pathogenic potential.

Key Words

tick-borne encephalitis virus strains pathogenic properties GAG inhibition 

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References

  1. 1.
    Belikov SI, Kondratov IG, Potapova UV, Leonova GN. The relationship between the structure of the tick-borne encephalitis virus strains and their pathogenic properties. PLoS One. 2014;9(4). ID e94946. doi:  https://doi.org/10.1371/journal.pone.0094946.
  2. 2.
    Germi R, Crance JM, Garin D, Guimet J, Lortat-Jacob H, Ruigrok RW, Zarski JP, Drouet E. Heparan sulfate-mediated binding of infectious dengue virus type 2 and yellow fever virus. Virol. 2002;292(1):162-168.CrossRefGoogle Scholar
  3. 3.
    Goto A, Hayasaka D, Yoshii K, Mizutani T, Kariwa H, Takashima I. A BHK-21 cell-culture-adapted tick-borne encephalitis virus mutant is attenuated for neuroinvasiveness. Vaccine. 2003;21(25-26):4043-4051.CrossRefGoogle Scholar
  4. 4.
    Hilgard P, Stockert R. Heparan sulfate proteoglycans initiate dengue virus infection of hepatocytes. Hepatology. 2000;32(5):1069-1077.CrossRefGoogle Scholar
  5. 5.
    Kozlovskaya LI, Osolodkin DI, Shevtsova AS, Romanova LIu, Rogova YV, Dzhivanian TI, Lyapustin VN, Pivanova GP, Gmyl AP, Palyulin VA, Karganova GG. GAG-binding variants of tick-borne encephalitis virus. Virology. 2010;398(2):262-272.CrossRefGoogle Scholar
  6. 6.
    Kroschewski H, Allison SL, Heinz FX, Mandl CW. Role of heparan sulfate for attachment of tick-borne encephalitis virus. Virology. 2003;308(1):92-100.CrossRefGoogle Scholar
  7. 7.
    Lee E, Wright PJ, Davidson A, Lobigs M. Virulence attenuation of Dengue virus due to augmented glycosaminoglycan-binding affinity and restriction in extraneural dissemination. J. Gen. Virol. 2006;87(Pt 10):2791-2801.CrossRefGoogle Scholar
  8. 8.
    Leonova GN, Maystrovskaya OS, Kondratov IG, Takashima I, Belikov SI. The nature of replication of tick-borne encephalitis virus strains isolated from residents of the Russian Far East with inapparent and clinical forms of infection. Virus Res. 2014;189:34-42.CrossRefGoogle Scholar
  9. 9.
    Luat le X, Tun MM, Buerano CC, Aoki K, Morita K, Hayasaka D. Pathologic potential of variant clones of the oshima strain of far-eastern subtype tick-borne encephalitis virus. Trop. Med. Health. 2014;42(1):15-23.CrossRefGoogle Scholar
  10. 10.
    Mandl CW, Kroschewski H, Allison SL, Kofler R, Holzmann H, Meixner T, Heinz FX. Adaptation of tick-borne encephalitis virus to BHK-21 cells results in the formation of multiple heparan sulfate binding sites in the envelope protein and attenuation in vivo. J. Virol. 2001;75(12):5627-5637.CrossRefGoogle Scholar
  11. 11.
    Okamoto K, Kinoshita H, Parquet Mdel C, Raekiansyah M, Kimura D, Yui K, Islam M.A, Hasebe F, Morita K. Dengue virus strain DEN2 16681 utilizes a specific glycochain of syndecan-2 proteoglycan as a receptor. J. Gen. Virol. 2012;93(Pt 4):761-770.CrossRefGoogle Scholar

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© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.G. P. Somov Research Institute of Epidemiology and MicrobiologyVladivostokRussia

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