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High-Throughput Platform for B-Cell Screening Based on Fluorescent Phage-Display Technology

  • Ya. A. LomakinEmail author
  • A. N. Kaminskaya
  • A. V. Stepanov
  • A. A. Shmidt
  • A. G. Gabibov
  • A. A. BelogurovJr
BIOPHYSICS AND BIOCHEMISTRY

A system for detection of malignantly transformed cells, including follicular lymphoma Bcells, was developed and experimentally validated. The system is based on the use of bacteriophages carrying exposed ligands for pathogenic B-cell receptors. The efficiency of binding to target cells is several times higher than in systems with chemically synthesized biotinylated peptides. The new method is proposed as a noninvasive diagnostic test for mapping B-cell lymphoma and for determining the specificity of B-cell receptors and high-throughput combinatorial selection of various repertories of B cells.

Key Words

M13K07 bacteriophage B-cell receptor phage display follicular lymphoma flow cytofluorometry 

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References

  1. 1.
    Samochatova EV. Federal Clinical Recommendations on Diagnostics and Treatment of Non-Hodgkin’s Lymphoma. Moscow, 2014. Russian.Google Scholar
  2. 2.
    Stepanov AV, Markov OV, Chernikov IV, Gladkikh DV, Zhang H, Jones T, Sen’kova AV, Chernolovskaya EL, Zenkova MA, Kalinin RS, Rubtsova MP, Meleshko AN, Genkin DD, Belogurov AA Jr, Xie J, Gabibov AG, Lerner RA. Autocrine-based selection of ligands for personalized CAR-T therapy of lymphoma. Sci. Adv. 2018;4(11). ID eaau4580. doi:  https://doi.org/10.1126/sciadv.aau4580.
  3. 3.
    Amanna IJ, Slifka MK. Quantitation of rare memory B cell populations by two independent and complementary approaches. J. Immunol. Methods. 2006;317(1-2):175-185.CrossRefGoogle Scholar
  4. 4.
    Choi JH, Lee WK, Han SH, Ha S, Ahn SM, Kang JS, Choi YJ, Yun CH. Identification and characterization of nonapeptide targeting a human B cell lymphoma, Raji. Int. Immunopharmacol. 2008;8(6):852-858.CrossRefGoogle Scholar
  5. 5.
    Freedman A. Follicular lymphoma: 2018 update on diagnosis and management. Am. J. Hematol. 2018;93(2):296-305.CrossRefGoogle Scholar
  6. 6.
    Hajitou A, Trepel M, Lilley CE, Soghomonyan S, Alauddin MM, Marini FC 3rd, Restel BH, Ozawa MG, Moya CA, Rangel R, Sun Y, Zaoui K, Schmidt M, von Kalle C, Weitzman MD, Gelovani JG, Pasqualini R, Arap W. A hybrid vector for ligand-directed tumor targeting and molecular imaging. Cell. 2006;125(2):385-398.CrossRefGoogle Scholar
  7. 7.
    Karimi P, Birmann BM, Anderson LA, McShane CM, Gadalla SM, Sampson JN, Mbulaiteye SM. Risk factors for Burkitt lymphoma: a nested case-control study in the UK Clinical Practice Research Datalink. Br. J. Haematol. 2018;181(4):505-514.CrossRefGoogle Scholar
  8. 8.
    Lackraj T, Goswami R, Kridel R. Pathogenesis of follicular lymphoma. Best Pract. Res. Clin. Haematol. 2018;31(1):2-14.CrossRefGoogle Scholar
  9. 9.
    Mendez M, Torrente M, Provencio M. Follicular lymphomas and their transformation: Past and current research. Expert Rev. Hematol. 2017;10(6):515-524.CrossRefGoogle Scholar
  10. 10.
    Shankland KR, Armitage JO, Hancock BW. Non-Hodgkin lymphoma. Lancet. 2012;380:848-857.CrossRefGoogle Scholar
  11. 11.
    Sheikhpour R, Pourhosseini F, Neamatzadeh H, Karimi R. Immunophenotype evaluation of Non-Hodgkin’s lymphomas. Med. J. Islam. Repub. Iran. 2017;31. ID 121. doi:  https://doi.org/10.14196/mjiri.31.121.
  12. 12.
    Smith MJ, Packard TA, O’Neill SK, Hinman RM, Rihanek M, Gottlieb PA, Cambier JC. Detection and enrichment of rare antigen-specific B cells for analysis of phenotype and function. J. Vis. Exp. 2017;(120). doi:  https://doi.org/10.3791/55382.
  13. 13.
    van den Brand M, Scheijen B, Hess CJ, van Krieken JHJ, Groenen PJTA. Pathways towards indolent B-cell lymphoma — Etiology and therapeutic strategies. Blood Rev. 2017;31(6):426-435.CrossRefGoogle Scholar
  14. 14.
    Weiner GJ, Link BK. Monoclonal antibody therapy of B cell lymphoma. Expert Opin. Biol. Ther. 2004;4(3):375-385.CrossRefGoogle Scholar
  15. 15.
    Xie XF, Shen YM, Yang XC, Dong NZ, Bai X, Shi YZ. Construction and screening of the specific Fab phage antibody library against Raji cell strain. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2007;23(2):160-163.Google Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Ya. A. Lomakin
    • 1
    • 2
    Email author
  • A. N. Kaminskaya
    • 1
  • A. V. Stepanov
    • 1
    • 2
  • A. A. Shmidt
    • 1
  • A. G. Gabibov
    • 1
    • 3
  • A. A. BelogurovJr
    • 1
    • 3
  1. 1.M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of SciencesMoscowRussia
  2. 2.Kazan (Volga region) Federal UniversityKazanRussia
  3. 3.M. V. Lomonosov Moscow State UniversityMoscowRussia

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