High-Throughput Platform for B-Cell Screening Based on Fluorescent Phage-Display Technology
A system for detection of malignantly transformed cells, including follicular lymphoma Bcells, was developed and experimentally validated. The system is based on the use of bacteriophages carrying exposed ligands for pathogenic B-cell receptors. The efficiency of binding to target cells is several times higher than in systems with chemically synthesized biotinylated peptides. The new method is proposed as a noninvasive diagnostic test for mapping B-cell lymphoma and for determining the specificity of B-cell receptors and high-throughput combinatorial selection of various repertories of B cells.
Key WordsM13K07 bacteriophage B-cell receptor phage display follicular lymphoma flow cytofluorometry
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