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Cell Sheets of Mesenchymal Stromal Cells Effectively Stimulate Healing of Deep Soft Tissue Defects

  • N. A. AleksandrushkinaEmail author
  • N. V. Danilova
  • O. A. Grigorieva
  • P. G. Mal’kov
  • V. S. Popov
  • A. Yu. Efimenko
  • P. I. Makarevich
Translated from Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine)
  • 6 Downloads

We used rat model of splinted defect of the skin and soft tissues to compare the efficiency f mesenchymal stromal cells applied in sheets or in suspension for the treatment of these injuries. Transplantation of mesenchymal stromal cells significantly accelerated wound healing in comparison with the control. In the group treated by application of mesenchymal stromal cell sheets, the defect was closed by day 28, in the group treated with cell suspension by day 35, and in the control group after 49 days. According to histological analysis of the tissue samples, the formation of the granulation tissue and fibrosis occurred earlier after application of mesenchymal stromal cells. Application of mesenchymal stromal cells in the form of cell sheets demonstrated high efficiency, which allowed us to consider this approach as a promising method of healing of skin and soft tissue injuries.

Key Words

cell sheet mesenchymal stromal cells skin wounds wound healing fibrosis 

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© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • N. A. Aleksandrushkina
    • 1
    • 2
    Email author
  • N. V. Danilova
    • 3
  • O. A. Grigorieva
    • 1
  • P. G. Mal’kov
    • 3
  • V. S. Popov
    • 2
  • A. Yu. Efimenko
    • 1
    • 2
  • P. I. Makarevich
    • 1
    • 2
  1. 1.Institute for Regenerative MedicinePittsburghUSA
  2. 2.Faculty of Fundamental MedicineM. V. Lomonosov Moscow State UniversityMoskvaRussia
  3. 3.Department of Clinical Pathology, Medical Scientific and Educational CenterM. V. Lomonosov Moscow State UniversityMoscowRussia

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