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Quantitative and Qualitative Characterization of Phagocytic Activity of Macrophages of Bone Marrow and Fetal Origin

  • A. V. Lokhonina
  • A. V. Makarov
  • A. V. ElchaninovEmail author
  • I. V. Arutyunyan
  • T. V. Shmakova
  • M. V. Grinberg
  • N. Yu. Usman
  • V. V. Surovtsev
  • V. P. Chernikov
  • T. Kh. Fatkhudinov
Translated from Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine)
  • 6 Downloads

We compared phagocytic activity of macrophages of monocyte origin and Kupffer cells under the influence of M1 and M2 inducers and without activation. Cultures of monocyte-derived macrophages and Kupffer cells were characterized by intensive expression of CD68 that was not affected by activation factors. At the same time, these cultures demonstrated different dynamics of phagocytic activity. Monocyte-derived macrophages initially had more pronounced absorption capacity that gradually increased during the experiment. Kupffer cells were characterized by abrupt fluctuations of phagocytic activity: sharp growth and rapid saturation. Despite these differences, the endosomes produced by monocyte-derived macrophages and Kupffer cells had similar degrees of maturity.

Key Words

macrophages Kupffer cells monocytes phagocytosis 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • A. V. Lokhonina
    • 1
    • 4
  • A. V. Makarov
    • 1
    • 3
  • A. V. Elchaninov
    • 1
    • 4
    Email author
  • I. V. Arutyunyan
    • 1
    • 2
  • T. V. Shmakova
    • 2
  • M. V. Grinberg
    • 4
  • N. Yu. Usman
    • 1
  • V. V. Surovtsev
    • 4
  • V. P. Chernikov
    • 2
  • T. Kh. Fatkhudinov
    • 1
    • 4
  1. 1.V. I. Kulakov National Medical Research Center for Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian FederationMoscowRussia
  2. 2.Research Institute of Human Morphology, Research Institute of Human MorphologyMoscowRussia
  3. 3.N. I. Pirogov Russian National Research Medical University, Ministry of Health of the Russian FederationMoscowRussia
  4. 4.Peoples’ Friendship University of RussiaMoscowRussia

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