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Isolation of IgG Associated with Human Placenta

  • N. V. Ignat’eva
  • M. M. ZiganshinaEmail author
  • N. V. Shilova
  • N. R. Khasbiullina
  • N. V. Bovin
  • V. L. Tyutyunnik
  • G. T. Sukhikh
Article
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We optimized the method of isolation of antibodies from placental tissue of a conventionally healthy patient. Four protocols of antibody isolation were evaluated and a protocol with tissue grinding (without homogenization) and successive elution of the antibodies with acidic and alkaline buffers was recommended for use. The repertoire of the isolated antibodies was characterized using a glycan array. Partial coincidence of the specificity of the isolated antibodies with antibodies in the peripheral blood was demonstrated, which indicates their possible association with carbohydrate antigens in the placenta. Identification of potential molecular targets of resident antibodies in the placenta is necessary for understanding the mechanisms of formation of immunological tolerance to the fetus.

Key Words

immunological tolerance placenta antiglycan antibodies immunoglobulins G glycan array 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • N. V. Ignat’eva
    • 1
  • M. M. Ziganshina
    • 1
    Email author
  • N. V. Shilova
    • 2
  • N. R. Khasbiullina
    • 2
  • N. V. Bovin
    • 2
  • V. L. Tyutyunnik
    • 1
  • G. T. Sukhikh
    • 1
  1. 1.V. I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian FederationMoscowRussia
  2. 2.M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of SciencesMoscowRussia

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