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Comparative Analysis of Blood Plasma Proteome in Patients with Renal Cell Carcinoma

  • A. L. KayshevaEmail author
  • A. T. Kopylov
  • N. E. Kushlinskii
  • E. S. Gershtein
  • A. A. Alferov
  • A. A. Morozov
  • I. A. Kazantseva
  • T. O. Pleshakova
  • A. I. Archakov
  • Yu. D. Ivanov
Article

Comparative mass spectrometric analysis of protein composition was carried out in 36 blood plasma specimens from patients with renal cell carcinoma and 20 specimens from donors. Analysis of protein composition of plasma specimens devoid of the major protein fractions showed a 20-50% higher level of protein identifications in patient’ specimens. Specimens of the control and experimental series were similar by protein composition, 70-80% identifications in experimental and control series coinciding. High similarity of biological processes with participation of the proteins identified in both series was observed. The greater part of proteins in both series were located extracellularly and were exosomal (specimens from renal cancer patients) or vesicular (specimens from healthy volunteers).

Key Words

renal cell carcinoma blood plasma liquid chromato-mass-spectrometry panoramic analysis 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • A. L. Kaysheva
    • 1
    Email author
  • A. T. Kopylov
    • 1
  • N. E. Kushlinskii
    • 2
  • E. S. Gershtein
    • 2
  • A. A. Alferov
    • 2
  • A. A. Morozov
    • 3
  • I. A. Kazantseva
    • 3
  • T. O. Pleshakova
    • 1
  • A. I. Archakov
    • 1
  • Yu. D. Ivanov
    • 1
  1. 1.V. N. Orekhovich Research Institute of Biomedical ChemistryMoscowRussia
  2. 2.N. N. Blokhin National Medical Center of Oncology, the Ministry of Health of RussiaMoscowRussia
  3. 3.M. F. Vladimirsky Moscow Regional Research and Clinical InstituteMoscowRussia

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