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Effect of Interleukin-10 on Localization of AMPA Receptors in Synapses during Long-Term Posttetanic Potentiation in Cultured Hippocampal Slices

  • I. B. MikheevaEmail author
  • A. E. Malkov
  • L. L. Pavlik
  • V. I. Arkhipov
  • S. G. Levin
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The effect of the anti-inflammatory cytokine IL-10 on the ultrastructural distribution of AMPA receptor GluR1 subunit in CA1 field of cultured hippocampal slices was studied by using immunohistochemical technique. It was found that long-term posttetanic potentiation increased the content of GluR1 in the postsynaptic density of the axo-spinous synapse. Addition of IL-10 in concentrations of 1 and 10 ng/ml to the medium facilitated long-term posttetanic potentiation thereby changing the distribution of GluR1 in the spine: the number of receptors increased in the cytoplasm and decreased in the postsynaptic density. It is assumed that activation of neuronal IL-10 receptors affects the distribution of AMPA receptors in axo-spinous synapses of hippocampal field CA1 through interplay of intracellular signaling pathways, thereby participating in the mechanisms of synaptic plasticity under normal conditions.

Key Words

interleukin-10 posttetanic potentiation immunocytochemistry GluR1 subunit of AMPA receptors 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • I. B. Mikheeva
    • 1
    Email author
  • A. E. Malkov
    • 1
  • L. L. Pavlik
    • 1
  • V. I. Arkhipov
    • 1
    • 2
  • S. G. Levin
    • 1
    • 2
  1. 1.Institute of Theoretical and Experimental BiophysicsRussian Academy of SciencesPushchinoRussia
  2. 2.Pushchino State Institute of Natural SciencesPushchinoRussia

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