Advertisement

Selective Action of N-Arachidonoyl Dopamine on Viability and Proliferation of Stromal Cells from Eutopic and Ectopic Endometrium

  • A. M. Ashba
  • M. N. Yushina
  • I. A. Fedorova-Gogolina
  • N. M. Gretskaya
  • V. V. Bezuglov
  • A. G. Melkumyan
  • S. V. Pavlovich
  • M. Yu. BobrovEmail author
Article

We performed a comparative study of the cytotoxic effect of endocannabinoid N-arachidonoyl dopamine (AA-DA) on cultured stromal cells of ectopic and eutopic endometrium. It was found that AA-DA in the concentration range of 1-20 μM produces more selective cytotoxic effect on the stromal cells of the ectopic endometrium due to interaction with cannabinoid type 1 receptor. In concentrations below 1 μM, AA-DA stimulated the proliferation of stromal cells of the eutopic endometrium and did not affect the division of ectopic endometrium cells. This effect was realized due to its interaction with cannabinoid type 2 receptor.

Key Words

endometriosis stromal cells N-arachidonoyl dopamine cytotoxicity proliferation 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Endometriosis: Diagnosis, Treatment and Rehabilitation. Federal Clinical Recommendations. Moscow, 2013. Russian.Google Scholar
  2. 2.
    Ashba AM, Akimov MG, Gretskaya NM, Bezuglov VV. N-acyl dopamines induce cell death in PC12 cell line via induction of nitric oxide generation and oxidative stress. Doklady Biochem. Biophysics. 2016;467(1):81-84.CrossRefGoogle Scholar
  3. 3.
    Bobrov MY, Lyzhin AA, Andrianova EL, Gretskaya NM, Zinchenko GN, Frumkina LE, Khaspekov LG, Bezuglov VV. Antioxidant and neuroprotective properties of N-docosahexaenoyl dopamine. Bull. Exp. Biol. Med. 2006;142(4):425-427.CrossRefGoogle Scholar
  4. 4.
    Chernukha GE, Ilyina LM, Adamyan LV, Pavlovich SV. Deep infiltrating endometriosis: Postoperative recurrences and possible ways of their prevention. Akush. Gin. 2015;(8):39-46. Russian.Google Scholar
  5. 5.
    Akimov MG, Ashba AM, Gretskaya NM, Bezuglov VV. Some aspects of the mechanism of cell death induction by the lipids of N-acyl dopamine family in the PC12 cancer cell line. Anticancer Res. 2014;34(10):5797-5800.Google Scholar
  6. 6.
    Akimov MG, Gretskaya NM, Zinchenko GN, Bezuglov VV. Cytotoxicity of Endogenous Lipids N-acyl Dopamines and their Possible Metabolic Derivatives for human cancer cell lines of different histological origin. Anticancer Res. 2015;35(5):2657-2661.Google Scholar
  7. 7.
    Andrianova EL, Bobrov MY, Gretskaya NM, Zinchenko GN, Fomina-Ageeva EV, Bezuglov VV, Serkov IV. The effects of neurolipins and their synthetic analogues on normal and transformed glial cells. Neurochem. J. 2010;4(1):46-54.CrossRefGoogle Scholar
  8. 8.
    Berkley KJ, Rapkin AJ, Papka RE. The pains of endometriosis. Science. 2005;308:1587-1589.CrossRefGoogle Scholar
  9. 9.
    Bobrov MY, Lizhin AA, Andrianova EL, Gretskaya NM, Frumkina LE, Khaspekov LG, Bezuglov VV. Antioxidant and neuroprotective properties of N-arachidonoyldopamine. Neurosci. Lett. 2008;431(1):6-11.CrossRefGoogle Scholar
  10. 10.
    Giudice LC, Kao LC. Endometriosis. Lancet. 2004;364:1789-1799.CrossRefGoogle Scholar
  11. 11.
    Liu X, Yamada N, Maruyama W, Osawa T. Formation of dopamine adducts derived from brain polyunsaturated fatty acids mechanism for Parkinson disease. J. Biol. Chem. 2008; 283(50):34,887-34,895.CrossRefGoogle Scholar
  12. 12.
    Schwarz R, Ramer R, Hinz B. Targeting the endocannabinoid system as a potential anticancer approach. Drug Metab. Rev. 2018;50(1):26-53.CrossRefGoogle Scholar
  13. 13.
    Sanchez AM, Cioffi R, Viganò P, Candiani M, Verde R, Piscitelli F, Di Marzo V, Garavaglia E, Panina-Bordignon P. Elevated systemic levels of endocannabinoids and related mediators across the menstrual cycle in women with endometriosis. Reprod. Sci. 2016;23(8):1071-1079.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • A. M. Ashba
    • 1
    • 2
  • M. N. Yushina
    • 1
  • I. A. Fedorova-Gogolina
    • 1
  • N. M. Gretskaya
    • 2
  • V. V. Bezuglov
    • 2
  • A. G. Melkumyan
    • 1
  • S. V. Pavlovich
    • 1
  • M. Yu. Bobrov
    • 1
    • 2
    Email author
  1. 1.V. I. Kulakov National Medical Research Center for Obstetrics, Gynecology, and PerinatologyMinistry of Health of the Russian FederationMoscowRussia
  2. 2.M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic ChemistryRussian Academy of SciencesMoscowRussia

Personalised recommendations