Administration of Protein Synthesis Inhibitor before Reminder Reverses Amnesia Induced by Memory Reconsolidation Impairment with 5-HT Receptors Antagonist

  • V. P. NikitinEmail author
  • S. A. Kozyrev
  • S. V. Solntseva

Administration of 5-HT receptor antagonist to snails trained in conditioned food aversion prior to reminding of the conditioning stimulus caused amnesia. At the early period of amnesia (day 3), injections of protein synthesis inhibitor cycloheximide without reminder or reminder alone were ineffective. At the same time, injections of the inhibitor combined with reminder led to memory recovery; this effect in most animals persisted for at least 10 days. In the rest snails, aversive responses to presentations of the conditioning stimulus persisted for 2 days. Cycloheximide injection and reminder in 10 days after induction of amnesia did not affect its development or caused a transient memory recovery (2 days). We hypothesized that amnesia is an active process unfolding in time. One of mechanism of this process is reminder-induced and protein synthesis-depended reactivation of amnesia. Inhibitor of protein synthesis disturbed this reactivation and led to recovery of the initial memory of conditioned food aversion.

Key Words

reconsolidation memory amnesia 5-HT receptors protein synthesis inhibitors 


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  1. 1.
    Amelchenko EM, Zworykina SV, Bezriadnov DV, Chekhov SA, Anokhin KV. Recovery of impaired memory and c-fos gene expression in brains of amnestic animals in response to reminder stimulation. Bull. Exp. Biol. Med. 2012;153(5):738-741.CrossRefGoogle Scholar
  2. 2.
    Solntseva SV, Nikitin VP. Induction of amnesia induced by disturbance of memory consolidation by antagonists of glutamate or serotonin receptors will be suppressed by the protein synthesis inhibitor. Ross. Fiziol. Zh. 2010;96(12):1212. Russian.Google Scholar
  3. 3.
    Solntseva SV, Nikitin VP. Induction of latent memory for conditioned food aversion and its transformation into “active” state depend on translation and transcription processes. Bull. Exp. Biol. Med. 2014;157(1):1-4.CrossRefGoogle Scholar
  4. 4.
    Alberini CM, Chen DY. Memory enhancement: consolidation, reconsolidation and insulin-like growth factor 2. Trend. Neurosci. 2012;35(5):274-283.CrossRefGoogle Scholar
  5. 5.
    Briggs JF, Olson BP. Reexposure to the amnestic agent alleviates cycloheximide-induced retrograde amnesia for reactivated and extinction memories. Learn. Mem. 2013;20(5):285-288.CrossRefGoogle Scholar
  6. 6.
    Finnie PS, Nader K. The role of metaplasticity mechanisms in regulating memory destabilization and reconsolidation. Neurosci. Biobehav. Rev. 2012;36(7):1667-1707.CrossRefGoogle Scholar
  7. 7.
    Gisquet-Verrier P, Lynch JF 3rd, Cutolo P, Toledano D, Ulmen A, Jasnow AM, Riccio DC. Integration of new information with active memory accounts for retrograde amnesia: a challenge to the consolidation/reconsolidation hypothesis? J. Neurosci. 2015;35(33):11,623-11,633.CrossRefGoogle Scholar
  8. 8.
    Gold PE. The many faces of amnesia. Learn. Mem. 2006; 13(5):506-514.CrossRefGoogle Scholar
  9. 9.
    Kiss T, Pirger Z, Kemenes G. Food-aversive classical conditioning increases a persistent sodium current in molluscan withdrawal interneurons in a transcription dependent manner. Neurobiol. Learn. Mem. 2009;92(1):114-119.CrossRefGoogle Scholar
  10. 10.
    Lee JL. Reconsolidation: maintaining memory relevance. Trends Neurosci. 2009;32(8):413-420.CrossRefGoogle Scholar
  11. 11.
    Menges SA, Riepe JR, Philips GT. Latent memory facilitates relearning through molecular signaling mechanisms that are distinct from original learning. Neurobiol. Learn. Mem. 2015;123:35-42.CrossRefGoogle Scholar
  12. 12.
    Nader K. New approaches to amnesia. Learn. Mem. 2009;16:672-675. doi: Scholar
  13. 13.
    Nikitin VP, Solntseva SV, Kozyrev SA, Nikitin PV, Shevelkin AV. Different components of conditioned food aversion memory. Brain Res. 2016;1642:104-113.CrossRefGoogle Scholar
  14. 14.
    Nikitin VP, Solntseva SV, Nikitin PV, Kozyrev SA. The role of DNA methylation in the mechanisms of memory reconsolidation and development of amnesia. Behav. Brain Res. 2015;279:148-154.CrossRefGoogle Scholar
  15. 15.
    Parvez K, Stewart O, Sangha S, Lukowiak K. Boosting intermediate-term into long-term memory. J. Exp. Biol. 2005;208(Pt 8):1525-1536.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • V. P. Nikitin
    • 1
    Email author
  • S. A. Kozyrev
    • 1
  • S. V. Solntseva
    • 1
  1. 1.P. K. Anokhin Research Institute of Normal PhysiologyMoscowRussia

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