Bulletin of Experimental Biology and Medicine

, Volume 166, Issue 3, pp 364–368 | Cite as

Post-Weaning Social Isolation Disturbs Gene Expression in Rat Brain Structures

  • E. A. Zubkov
  • Ya. A. Zorkina
  • E. V. Orshanskaya
  • N. N. Khlebnikova
  • N. A. KrupinaEmail author
  • V. P. Chekhonin

Post-weaning social isolation of male Wistar rats for 10 weeks led to an increase of their aggressiveness, sensorimotor reactivity, and cognitive deficiency, manifesting in training disorders evaluated by the acoustic startle response (amplitude of the response decreasing). Expression of gene encoding serine protease prolyl endopeptidase (EC in the frontal cortex was higher than in control rats kept in groups, while the level of mRNA of the gene encoding dipeptidyl peptidase IV (EC did not differ from the control in any of the brain structures. The levels of serotonin transporter gene mRNA in the striatum and hypothalamus were higher than in the control. No appreciable changes in the expression of genes encoding tryptophan hydroxylase-2 and monoaminoxidase A and B in the frontal cortex, striatum, amygdala, hypothalamus, and hippocampus were detected. The data indicated the involvement of genes associated with the serotoninergic system in the mechanisms of mental disorders induced by post-weaning social isolation and suggest the gene encoding prolyl endopeptidase as a candidate gene involved in the pathogenesis of these disorders.

Key Words

animal model post-weaning social isolation rats gene expression brain structures 


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© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • E. A. Zubkov
    • 1
  • Ya. A. Zorkina
    • 1
  • E. V. Orshanskaya
    • 2
  • N. N. Khlebnikova
    • 3
  • N. A. Krupina
    • 3
    Email author
  • V. P. Chekhonin
    • 1
  1. 1.Department of Basic and Applied Neurobiology, V. P. Serbsky National medical Research Center of Psychiatry and NarcologyMinistry of Health of Russian FederationMoscowRussia
  2. 2.Laboratory of General Pathology of the Cardiorespiratory SystemMoscowRussia
  3. 3.Laboratory of General Pathology of the Nervous SystemResearch Institute of General Pathology and PathophysiologyMoscowRussia

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