Semaphorin 3A Negatively Affects Proliferation of Mouse Thymus Epithelial Cells In Vitro
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We studied effects of semaphorin 3A, keratinocyte growth factor (KGF), hepatocyte growth factor (HGF), and their combinations on the proliferative activity of cortical (cTEC1-2) and medullary (mTEC3-10) thymus epithelium cell lines. Semaphorin 3A inhibited the proliferative activity of epithelial cells, while HGF and KGF, in contrast, exerted a stimulating effect. The effect of KGF and semaphorin 3A on different cell lines depended on the expression of receptors for these two factors. When the combination of two factors was used, semaphorin 3A was able to neutralize the stimulating effect of HGF and KGF. It can be assumed that semaphorin 3A synthesized in the thymus stroma, can act as a functional antagonist of HGF and KGF and have an inhibitory effect when these drugs are administered into the body for the therapeutic purpose of restoring thymus functions.
Key Wordssemaphorin 3A keratinocyte growth factor (KGF) hepatocyte growth factor (HGF) thymus epithelial cells proliferation
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- 1.Kisseleva EP, Lyamina IV, Tzvirkun SA, Rutto KV, Kudryavtsev IV, Lioudyno VI. Investigation of the role of semaphorin 3a in the thymus at normal conditions and tumor growth. Med. Akad. Zh. 2013;13(4):42-48. Russian.Google Scholar
- 12.Rossi SW, Jeker LT, Ueno T, Kuse S, Keller MP, Zuklys S, Gudkov AV, Takahama Y, Krenger W, Blazar BR, Holländer GA. Keratinocyte growth factor (KGF) enhances postnatal T-cell development via enhancements in proliferation and function of thymic epithelial cells. Blood. 2007;109(9):3803-3811.CrossRefGoogle Scholar