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Bulletin of Experimental Biology and Medicine

, Volume 166, Issue 3, pp 317–320 | Cite as

Functional State of Various Types of Regeneration-Competent Cells in the Nervous Tissue in Ethanol-Induced Neurodegeneration

  • G. N. Zyuz’kovEmail author
  • L. A. Miroshnichenko
  • E. V. Udut
  • A. V. Chaikovskii
  • T. Yu. Polyakova
  • E. V. Simanina
  • L. A. Stavrova
  • V. I. Agafonov
  • V. V. Zhdanov
GENERAL PATHOLOGY AND PATHOPHYSIOLOGY
  • 29 Downloads

The in vitro and in vivo models of ethanol-induced neurodegeneration were used to evaluate the content and functional activity of various types of regeneration-competent cells in subventricular zone of the cerebral hemispheres in C57Bl/6JY mice. In nervous tissue culture, ethanol (65 mM) produced no effect on formation of neurospheres. When administered per os in a daily dose of 3 g/kg for 8 weeks, ethanol produced no effect on the number of neural CFU in situ. In both cases, ethanol reduced proliferative activity of neural CFU. Long-term administration of ethanol in vivo suppressed differentiation of neural stem cells and decreased the number of committed precursors (neural cluster-forming units) in the subventricular zone of cerebral hemispheres. In vitro application of ethanol stimulated secretion of humoral growth factors by the cluster-forming neural glial cells. In contrast, in vivo administration of ethanol suppressed this secretion.

Key Words

ethanol-induced neurodegeneration neural stem cells glial cells regeneration medicine neuroprotection 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • G. N. Zyuz’kov
    • 1
    Email author
  • L. A. Miroshnichenko
    • 1
  • E. V. Udut
    • 1
  • A. V. Chaikovskii
    • 1
  • T. Yu. Polyakova
    • 1
  • E. V. Simanina
    • 1
  • L. A. Stavrova
    • 1
  • V. I. Agafonov
    • 1
  • V. V. Zhdanov
    • 1
  1. 1.E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk National Research Medical CenterRussian Academy of SciencesTomskRussia

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