Expression of Bcl-2 Family Proteins in the Ovarian Follicular Apparatus in the Acute Period after Experimental Hyperthermia

  • S. V. Michurina
  • S. I. Kolesnikov
  • A. L. Bochkareva
  • S. A. Arkhipov
  • I. Yu. Ishchenko
MORPHOLOGY AND PATHOMORPHOLOGY

The expression of apoptosis regulators (proapoptotic protein Bad and anti-apoptotic protein Bcl-2) was analyzed and Bcl-2/Bad ratio in the follicular apparatus of the rat ovary was determined on day 3 after hyperthermia (rectal temperature 43.5°C). Hyperthermia in the catabolic phase leads to different degrees of activation of the molecular “switches” of apoptosis in cells of ovarian follicular epithelium. This was seen from increased intensity of immunohistochemical staining for Bad protein against the background of more pronounced expression of Bcl-2 protein. On day 3 after exposure to hyperthermia, Bcl-2/Bad ratio increased, which reflects antiapoptotic protection of cells and conditions for blockade of mitochondrial pathway of apoptosis in the follicular apparatus of the ovaries during the acute period after hyperthermia.

Key Words

apoptosis hyperthermia ovarian follicular apparatus Bcl-2/Bad 

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References

  1. 1.
    Michurina SV, Arkhipov SA, Kolesnikov SI. Hepatocyte apoptosis in rats exposed to benzo(a)pyrene. Bull. Exp. Biol. Med. 2014;158(1):150-152.CrossRefPubMedGoogle Scholar
  2. 2.
    Michurina SV, Bochkareva AL, Belkin AD, Zhdanov AP, Bochkarev IG. Morphometric analysis of the vascular bed and yellow bodies in the ovaries at different times after the general controlled hyperthermia (experimental study). Khirurg. 2008;(6):9-13. Russian.Google Scholar
  3. 3.
    Efremov AV, Pakhomova JuV, Pakhomov EA, Ibragimov RSh, Shorina GN. Patent RU No. 2165105. Method for carrying out experimental general hyperthermy modeling in small laboratory animals. Bull. No. 10. Published April 10, 2001.Google Scholar
  4. 4.
    Aroyo A, Yavin S, Arav A, Roth Z. Maternal hyperthermia disrupts developmental competence of follicle-enclosed oocytes: in vivo and ex vivo studies in mice. Theriogenology. 2007;67(5):1013-1021.CrossRefPubMedGoogle Scholar
  5. 5.
    Begriche K, Massart J, Robin MA, Borgne-Sanchez A, Fromenty B. Drug-induced toxicity on mitochondria and lipid metabolism: mechanistic diversity and deleterious consequences for the liver. J. Hepatol. 2011;54(4):773-794.CrossRefPubMedGoogle Scholar
  6. 6.
    Belzacq AS, Vieira HL, Verrier F, Vandecasteele G, Cohen I, Prévost MC, Larquet E, Pariselli F, Petit PX, Kahn A, Rizzuto R, Brenner C, Kroemer G. Bcl-2 and Bax modulate adenine nucleotide translocase activity. Cancer Res. 2003;63(2):541-546.PubMedGoogle Scholar
  7. 7.
    Bridges PJ, Brusie MA, Fortune JE. Elevated temperature (heat stress) in vitro reduces androstenedione and estradiol and increases progesterone secretion by follicular cells from bovine dominant follicles. Domest. Anim. Endocrinol. 2005;29(3):508-522.CrossRefPubMedGoogle Scholar
  8. 8.
    Dewanjee S, Dua TK, Khanra R, Das S, Barma S, Joardar S, Bhattacharjee N, Zia-Ul-Haq M, Jaafar HZ. Water Spinach, Ipomoea aquatic (Convolvulaceae), Ameliorates Lead Toxicity by Inhibiting Oxidative Stress and Apoptosis. PLoS One. 2015;10(10):e0139831. doi:  https://doi.org/10.1371/journal.pone.0139831.CrossRefPubMedPubMedCentralGoogle Scholar
  9. 9.
    Roth Z, Aroyo A, Yavin S, Arav A. The antioxidant epigallocatechin gallate (EGCG) moderates the deleterious effects of maternal hyperthermia on follicle-enclosed oocytes in mice. Theriogenology. 2008;70(6):887-897.CrossRefPubMedGoogle Scholar
  10. 10.
    Stankiewicz AR, Lachapelle G, Foo CP, Radicioni SM, Mosser DD. Hsp70 inhibits heat-induced apoptosis upstream of mitochondria by preventing Bax translocation. J. Biol. Chem. 2005;280(46):38 729-38 739.Google Scholar
  11. 11.
    Sun D, Li S, Wu H, Zhang M, Zhang X, Wei L, Qin X, Gao E Oncostatin M (OSM) protects against cardiac ischaemia/reperfusion injury in diabetic mice by regulating apoptosis, mitochondrial biogenesis and insulin sensitivity. J. Cell. Mol. Med. 2015;19(6):1296-1307.Google Scholar
  12. 12.
    Tseng JK, Tang PC, Ju JC. In vitro thermal stress induces apoptosis and reduces development of porcine parthenotes. Theriogenology. 2006;66(5):1073-1082.Google Scholar
  13. 13.
    Uchida S, Hotta H, Hanada T, Okuno Y, Aikawa Y. Effects of thermal stimulation, applied to the hindpaw via a hot water bath, upon ovarian blood flow in anesthetized nonpregnant rats. J. Physiol. Sci. 2007;57(4):227-233.CrossRefPubMedGoogle Scholar
  14. 14.
    Wang Y, Chen Y, Zhang X, Cai G, An S, Wang X, Teng L, Wang D. Tricholoma matsutake Aqueous Extract Induces Hepatocellular Carcinoma Cell Apoptosis via Caspase-Dependent Mitochondrial Pathway. Biomed. Res. Int. 2016;2016:9014364. doi:  https://doi.org/10.1155/2016/9014364. PubMedPubMedCentralGoogle Scholar
  15. 15.
    Yuan Y, Hao ZD, Liu J, Wu Y, Yang L, Liu GS, Tian JH, Zhu SE, Zeng SM. Heat shock at the germinal vesicle breakdown stage induces apoptosis in surrounding cumulus cells and reduces maturation rates of porcine oocytes in vitro. Theriogenology. 2008;70(2):168-178.Google Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • S. V. Michurina
    • 1
  • S. I. Kolesnikov
    • 2
  • A. L. Bochkareva
    • 1
  • S. A. Arkhipov
    • 1
  • I. Yu. Ishchenko
    • 1
  1. 1.Research Institute of Clinical and Experimental Lymphology, Affiliated Branch of the Federal Research Centre Institute of Cytology and GeneticsSiberian Division of the Russian Academy of ScienceNovosibirskRussia
  2. 2.Research Center for Family Health and Human Reproduction ProblemsIrkutskRussia

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