Effect of Oxidized Dextran on Cytokine Production and Activation of IRF3 Transcription Factor in Macrophages from Mice of Opposite Strains with Different Sensitivity to Tuberculosis Infection
We studied differences in the production of pro- and anti-inflammatory cytokines and IRF3 transcription factor by peritoneal macrophages from mice of opposite strains CBA/J and C57Bl/6 and the effect of 60-kDa oxidized dextran on these parameters. Macrophages from C57Bl/6 mice were mainly characterized by the production of proinflammatory cytokines TNFα, IL-12, and MCP-1 (markers of M1 polarization). By contrast, CBA/J mice exhibited a relatively high level of anti-inflammatory cytokine IL-10 and lower expression of proinflammatory cytokines (M2 phenotype). IRF3 content in peritoneal macrophages of CBA/J mice was higher than in C57Bl/6 mice. Oxidized dextran decreased the expression of IRF3 upon stimulation of cells from CBA/J mice with LPS, but increased this process in C57Bl/6 mice. Despite a diversity of oxidized dextran-induced changes in cytokine production, the data confirm our hypothesis that this agent can stimulate the alternative activation of macrophages.
Key Words60-kDa oxidized dextran peritoneal macrophages cytokines C56Bl/6 CBA/J
Unable to display preview. Download preview PDF.
- 3.Shkurupiy VA. The Ultrastructure of Liver Cells in Stress. Novosibirsk, 1989. Russian.Google Scholar
- 4.Shkurupiy VA, Chernova TG, Kurunov YuN. Granuloma changes in the treatment of tuberculosis with the prolonged form of isoniazid in the experiment. Probl. Tuberkul. Bol. Legkikh. 1993;(1):38-41. Russian.Google Scholar
- 5.Borges TK, Alves ÉA, Vasconcelos HA, Carneiro FP, Nicola AM, Magalhães KG, Muniz-Junqueira MI. Differences in the modulation of reactive species, lipid bodies, cyclooxygenase-2,5-lipoxygenase and PPAR-γ in cerebral malaria-susceptible and resistant mice. Immunobiology. 2017;222(4):604-619.CrossRefPubMedGoogle Scholar
- 9.Novikov A, Cardone M, Thompson R, Shenderov K, Kirschman KD, Mayer-Barber KD, Myers T.G, Rabin RL, Trinchieri G, Sher A, Feng CG. Mycobacterium tuberculosis triggers host type I IFN signaling to regulate IL-1β production in human macrophages. J. Immunol. 2011;187(5):2540-2547.Google Scholar
- 10.Probst CM, Silva RA, Menezes JP, Almeida TF, Gomes IN, Dallabona AC, Ozaki LS, Buck GA, Pavoni DP, Krieger MA, Veras PS. A comparison of two distinct murine macrophage gene expression profiles in response to Leishmania amazonensis infection. BMC Microbiol. 2012;12:22. doi: https://doi.org/10.1186/1471-2180-12-22.CrossRefPubMedPubMedCentralGoogle Scholar
- 11.Quigley J, Hughitt VK, Velikovsky CA, Mariuzza RA, El-Sayed NM, Briken V. The cell wall lipid PDIM contributes to phagosomal escape and host cell exit of Mycobacterium tuberculosis. MBio. 2017;8(2). pii: e00148-17. doi: 10.1128/mBio.00148-17.Google Scholar