Bulletin of Experimental Biology and Medicine

, Volume 164, Issue 4, pp 523–527 | Cite as

Preparation of Human Olfactory Ensheathing Cells for the Therapy of Spinal Cord Injuries

  • А. D. Voronova
  • O. V. Stepanova
  • M. P. Valikhov
  • A. V. Chadin
  • А. S. Dvornikov
  • I. V. Reshetov
  • V. P. Chekhonin
CELL TECHNOLOGIES IN BIOLOGY AND MEDICINE
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We developed an optimal protocol for preparing and culturing of olfactory ensheathing cells from human olfactory mucosa. Using this protocol, we obtained a culture enriched with human olfactory ensheathing cells. Immunofluorescence analysis by simultaneous expression of GFAP and p75NTR markers showed that the content of ensheathing cells was maximum in passage 3 and 4 cultures (94 and 89.5%, respectively). The developed protocol can be recommended for obtaining autologous preparations of human ensheathing cells for cell therapy of spinal cord injuries.

Key Words

olfactory ensheathing cells from human olfactory mucosa human olfactory mucosa cell therapy spinal cord injuries 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • А. D. Voronova
    • 1
    • 2
  • O. V. Stepanova
    • 2
  • M. P. Valikhov
    • 2
  • A. V. Chadin
    • 2
  • А. S. Dvornikov
    • 2
  • I. V. Reshetov
    • 3
  • V. P. Chekhonin
    • 1
    • 2
  1. 1.Department of Fundamental and Applied Neurobiology, V. P. Serbsky Federal Medical Research Centre for Psychiatry and NarcologyMinistry of Health of the Russian FederationMoscowRussia
  2. 2.Department of Medical Nanobiotechnologies, Medical and Biological FacultyN. I. Pirogov National Research Medical UniversityMoscowRussia
  3. 3.University Clinical Hospital No. 1, I. M. Sechenov First Moscow State Medical UniversityMinistry of Health of the Russian FederationMoscowRussia

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