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Effects of commercial superzist probiotic on growth performance and hematological and immune indices in fingerlings Acipenser baerii

  • Mir Hamed Sayed HassaniEmail author
  • Ayoub Yousefi Jourdehi
  • Ali Hosseinpour Zelti
  • Alireza Shenavar Masouleh
  • Frozan Bagherzadeh Lakani
Article
  • 30 Downloads

Abstract

This study was carried out to evaluate the effects of using superzist probiotic (a mixture of Lactobacillus spp., Bacillus subtilis, and Bifidobacterium bifidum) on growth performance and hematological and some immunological indices in fingerling Acipenser baerii. In total, 240 Acipenser baerii fingerlings with mean weight 10.5 ± 0.14 g−1 were stocked in 12 tanks, 20 per each tank and each treatment in triplicate. Diets were prepared by spraying slowly the mixture of 50 ml saline serum with 100, 200, and 300 mg probiotic powder per 1-kg diet to make the concentrations 1 × 106, 2 × 106, and 3 × 106 CFU g−1 of probiotic bacteria in diet. Results showed that there was a significant increase in the final weight, weight gain (WG), percentage weight gain, condition factor (CF), and specific growth rate (SGR) in fish fed PB300 treatment compared with the control at the 8th week (p < 0.05); also, the lowest feed conversion ratio (FCR) belonged to fish fed PB300 that showed a significant difference compared with fish fed control diet (p < 0.05). Except for neutrophil, lymphocyte, and hematocrit (p < 0.05), values of all hematological parameters of fish fed different concentrations of probiotic diet did not differ from the values of fish fed the control diet (p > 0.05), but there was a significant increase in lysozyme and IgM fish fed probiotic (PB300) and compliment (ACH50) (PB200 and PB300) compared with control treatment during 56 days. Therefore, results indicated that 300 mg kg−1 probiotic (Lactobacillus and Bifidobacterium) can be used as a proper probiotic in sturgeon aquaculture to enhance fish health and growth performance.

Keywords

Acipenser baerii Probiotic Growth rate Hematological indices Immune system 

Notes

Acknowledgments

The authors thank the experts and directors in International Sturgeon Research Institute (Iran) for cooperation, especially Alireza Alipour, Reza Ghorbani, Ali Hoshyar, and Arash Ghorbani.

Compliance with ethical standards

All applicable international, national, and/or institutional guidelines for the care and use of animals were followed by the authors.

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer Nature Switzerland AG 2019

Authors and Affiliations

  1. 1.Aquaculture Department of International Sturgeon Research Institute of Caspian SeaAgricultural Research Education and Extension Organization (AREEO)RashtIran

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