Aquaculture International

, Volume 27, Issue 6, pp 1859–1868 | Cite as

Characterization of the immunological factors in turbot (Scophthalmus maximus) seminal plasma and their correlation with sperm quality

  • Ming Ming Han
  • Fu Hong Ding
  • Jun LiEmail author


In this study, the concentration/activity of the immune components in the turbot (Scophthalmus maximus) seminal plasma at four time points with the intervals of 2 weeks has been summarized. The results showed that the concentrations of transferrin (TF), transforming growth factor-βs (TGF-βs), complement 3 (C3), IgM and interferon-1β (IL-1β) varied as experiment went on; it was the same with the activities of protease, lactate dehydrogenase and lysozyme. The correlation analysis showed that C3 and IgM were most positively related to motility and straight-line velocity (VSL)/curvilinear velocity (VCL), whereas protease was most negatively correlated with motility and VSL/VCL. This study highlighted the importance in the necessity and possibility in improving the sperm quality during the actual production.


Turbot Seminal plasma Immune factor Sperm quality 



Seminal plasma proteins


Reactive oxygen species




Super oxide dismutase


Glutathione reductase


Lipid peroxidation


Transforming growth factor-β1


Complement 3


Straight-line velocity


Curvilinear velocity


Lactic acid



We are grateful to Qingdao General Aquaculture Co. Ltd. for providing us the male bloodstock of turbots used in this experiment, and we thank the staff for their assistance in collecting semen.

Funding information

The whole study was funded by the Modern Agriculture Industry System Construction of Special Funds (CARS-50), National Natural Science Fund of China (3110188) and Provincial Natural Science Foundation of Shandong.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

All applicable international, national and/or institutional guidelines for the care and use of animals were followed by the authors.


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Copyright information

© Springer Nature Switzerland AG 2019

Authors and Affiliations

  1. 1.CAS Key Laboratory of Experimental Marine Biology, Institute of OceanologyChinese Academy of ScienceQingdaoPeople’s Republic of China
  2. 2.Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and TechnologyQingdaoPeople’s Republic of China
  3. 3.Center for Ocean Mega-ScienceChinese Academy of ScienceQingdaoPeople’s Republic of China
  4. 4.Yellow Sea Fisheries Research InstituteChinese Academy of Fishery ScienceQingdaoPeople’s Republic of China

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