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Aquaculture International

, Volume 27, Issue 6, pp 1813–1824 | Cite as

Multienzymatic capacity of cultivable intestinal bacteria from captive Litopenaeus vannamei (Boone, 1931) shrimp reared in green water

  • Jamille Martins Forte
  • Luiz Fagner Ferreira Nogueira
  • Rafael dos Santos Rocha
  • Rodrigo MaggioniEmail author
  • Oscarina Viana de Sousa
Article
  • 52 Downloads

Abstract

Equilibrium between the gastrointestinal bacterial population and the environment is a critical factor for the health of captive aquatic animals. The bacterial enzymes are fundamental for proper nutrition and pathogen resistance in shrimp. Therefore, enzymatic profiles reveal essential characteristics for the selection of probiotic strains that can improve animal development. In this work, we analysed shrimp from a green water system where infectious myonecrosis virus (IMNV) was present. We isolated transient and intestine resident bacterial populations, characterising eight functional groups through different culture media. To identify each isolated bacteria, we used sequences from regions V6–V8 of the 16S rRNA. To determine viral load of shrimp samples, we used real-time PCR. The number of colony forming units (CFU) was similar between IMNV-infected and IMNV-uninfected shrimps. The growth of transient bacteria was higher than the growth of resident. In general, lipolytic bacteria presented higher frequency and genus diversity than the other functional groups. All groups showed higher frequency among transitory bacteria, except the amylolytic functional group, which was more frequent among the resident. We found two major orders of cultivable bacteria, Vibrionales and Bacillales. The genus Vibrio was predominant among the Vibrionales, while Staphylococcus and Bacillus were the most frequent among the Bacillales. Recorded Vibrionales and Bacillales included pathogenic and beneficial species of high importance for aquaculture. The results presented here will serve as a basis for improving the nutritional and health conditions of Litopenaeus vannamei in green water farming systems.

Keywords

Litopenaeus vannamei Gut bacteria Green water IMNV Enzymatic group Probiotics 

Abbreviations

IMNV

Infectious myonecrosis virus

WSSV

White spot syndrome virus

CFU

Colony forming unit

CHB

Cultivable heterotrophic bacteria

Notes

Acknowledgments

We would like to thank the team at Laboratory of Environmental Microbiology and Fish (LAMAP, UFC) for the help in carrying out all the microbiological analysis processes. The authors kindly thank Ms. Kellie Johns, from James Cook University, for English revisions.

Funding

This work was funded by CAPES (CIMAR 2000/2014). J. Forte was supported by a CAPES PhD grant from CIMAR 2000/2014.

Compliance with ethical standards

All shrimp were collected and maintained in accordance with ethical standards in animal research.

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

10499_2019_431_MOESM1_ESM.docx (44 kb)
ESM 1 (DOCX 43 kb)

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© Springer Nature Switzerland AG 2019

Authors and Affiliations

  1. 1.LABOMAR Instituto de Ciências do Marinhas TropicaisUniversidade Federal do CearáFortalezaBrazil
  2. 2.Departamento de Engenharia de PescaUniversidade Federal do CearáFortalezaBrazil

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