Aquaculture International

, Volume 27, Issue 6, pp 1739–1749 | Cite as

Molecular identification of water molds (oomycetes) associated with chum salmon eggs from hatcheries in Japan and possible sources of their infection

  • Sakiko Orui Sakaguchi
  • Gen Ogawa
  • Hiroaki Kasai
  • Yuichi Shimizu
  • Hiroshi Kitazato
  • Katsunori Fujikura
  • Kiyotaka TakishitaEmail author


Oomycete infection of various freshwater animals, including salmonid eggs, causes significant economic damage to aquaculture worldwide. In this study, we detected oomycetes in infected chum salmon Oncorhynchus keta eggs at two hatcheries in northern Japan, in the source water used for egg incubation, and in the air at the hatcheries to clarify the source(s) of oomycete transmission using a DNA molecular marker. Seven oomycete taxa, belonging to Saprolegniaceae and Pythiaceae, were detected from the infected eggs. From the source water used for egg incubation and the air at the hatcheries, nine oomycete taxa, including those found on infected eggs, were detected, suggesting that both water and air are potential sources of oomycete transmission. There is no report of airborne transmission of these oomycetes detected in this study so far. Regarding protection and sterilization against oomycete infection in aquaculture hatcheries, not only water used at hatcheries but also the air in hatcheries may need to be considered hereafter.


Oomycetes Chum salmon eggs Saprolegniaceae Pythiaceae Internal transcribed spacer 2 



We thank the staffs of the two salmon hatcheries for their help with sample collection and Ms. Midori Hagio for DNA analyses.

Funding information

This study was supported by the research project Tohoku Ecosystem-Associated Marine Sciences from the Ministry of Education, Culture, Sports, Science, and Technology.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

All applicable international, national, and/or institutional guidelines for the care and use of animals were followed by the authors.


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Copyright information

© Springer Nature Switzerland AG 2019

Authors and Affiliations

  • Sakiko Orui Sakaguchi
    • 1
  • Gen Ogawa
    • 2
    • 3
  • Hiroaki Kasai
    • 4
  • Yuichi Shimizu
    • 2
  • Hiroshi Kitazato
    • 1
  • Katsunori Fujikura
    • 1
  • Kiyotaka Takishita
    • 1
    • 5
    Email author
  1. 1.Japan Agency for Marine-Earth Science and TechnologyYokosukaJapan
  2. 2.Iwate Fisheries Technology CenterIwateJapan
  3. 3.Iwate Prefectural Government Department of Agriculture, Forestry and FisheriesIwateJapan
  4. 4.School of Marine BioscienceKitasato University, 1-15-1 Kitasato, Minami-kuSagamiharaJapan
  5. 5.International College of Arts and SciencesFukuoka Women’s UniversityFukuokaJapan

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