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Aquaculture International

, Volume 27, Issue 5, pp 1535–1551 | Cite as

Influence of mannan oligosaccharide supplementation on haematological and immunological responses and disease resistance of striped catfish (Pangasianodon hypophthalmus Sauvage, 1878) juveniles

  • Mst. Nahid AkterEmail author
  • Roshada Hashim
  • Amalia Sutriana
  • Siti Azizah Mohd Nor
Article
  • 31 Downloads

Abstract

This study was carried out to evaluate the effect of mannan oligosacharide (MOS) as dietary supplement on haematological parameters, immune response and protection against Aeromonas hydrophila in striped catfish (Pangasianodon hypophthalmus, Sauvage, 1878) juveniles. Triplicate groups of juvenile striped catfish (initial weight 20.41 ± 1.64 g) were fed twice per day at 2.5% of body weight for 12 weeks, with 0 (control), 0.2, 0.4, 0.6 or 0.8% MOS diets. At the end of the feeding trial, fish were intraperitoneally challenged with 1 × 106 CFU ml−1of A. hydrophila and mortalities were recorded over 21 days. The haematological and immunological parameters were assessed both in pre- and post-challenged fish groups. Dietary inclusion of MOS did not show any significant influence on the majority of the haematological parameters of striped catfish when they were not infected. But it showed a significant positive influence on some of the most important parameters, namely RBC, WBC, lymphocyte, granulocyte and lysozyme activity and survival, after a 2-week challenge with A. hydrophila. After 2-week infection with A. hydrophila, a better concentration of red blood cell (RBC), white blood cell (WBC), granulocyte, total Ig content, lysozyme activity and survival were observed when striped catfish were fed with MOS at 0.4% and above, with significantly highest at 0.6% MOS diet than the control diet. Based on the results of this study, it is concluded that supplementation of MOS at least 0.4% is sufficient to activate the immune response and makes the striped catfish more resistant to A. hydrophila infection.

Keywords

Immune response Haematological parameters Mannan oligosaccharide P. hypophthalmus A. hydrophila 

Abbreviations

MOS

Mannan oligosaccharide

FOS

Fructo oligosaccharides

scFOS

Short-chain fructo oligosaccharides

GOS

Galacto oligosaccharides

ESR

Erythrocyte sedimentation rate

PCV

Packed cell volume

Hb

Haemoglobin

RBC

Red blood cell

WBC

White blood cell

MCHC

Mean corpuscular haemoglobin concentration

MCH

Mean corpuscular haemoglobin

MCV

Mean corpuscular volume

NaCl

Sodium chloride

Ig

Immunoglobulin

PEG

Polyethylene glycol

ANOVA

Analysis of variance

SD

Standard deviation

Notes

Funding information

This study was financially supported by the Organization for Women in Science in the Developing World (OWSDW), Exploratory Research Grant Scheme (ERGS), the Ministry of Higher Education, Malaysia (Project No.: 203 PBIOLOGI.6730134) and the Postgraduate Research Grant Scheme in USM (PRGS) for funding this research.

Compliance with ethical standards

Conflict of Interest

Author A. Mst. Nahid Akter has received a grant from the Organization for Women in Science in the Developing World (OWSDW) as research fellow and a research grant from the Postgraduate Research Grant Scheme (PRGS) in USM ; author B. Roshada Hashim received a grant from the Ministry of Higher Education, Malaysia, and the Exploratory Research Grant Scheme (ERGS) as mentor.

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Copyright information

© Springer Nature Switzerland AG 2019

Authors and Affiliations

  1. 1.Department of Aquaculture, Faculty of FisheriesHajee Mohammad Danesh Science and Technology UniversityDinajpurBangladesh
  2. 2.Faculty of Science and TechnologyUniversiti Sains Islam MalaysiaBandar Baru NilaiMalaysia
  3. 3.Faculty of Veterinary MedicineSyiah Kuala UniversityAcehIndonesia
  4. 4.Institute of Marine BiotechnologyUniversiti Malaysia TerengganuKuala TerengganuMalaysia

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