Aquaculture International

, Volume 27, Issue 5, pp 1513–1524 | Cite as

Isolation and characterization of heterotrophic nitrification–aerobic denitrification and sulphur-oxidizing bacterium Paracoccus saliphilus strain SPUM from coastal shrimp ponds

  • Y. D. JafferEmail author
  • H. Sanath Kumar
  • R. Vinothkumar
  • A. B. Irfan
  • N. M. Ishfaq
  • Parvaiz Ahmad Ganie
  • Raja Adil Hussain Bhat
  • A. Vennila


The Paracoccus sp. is one of the best-characterized prokaryote that has served as a model organism to study the dentrification and sulphur oxidation processes. However, its ability of dentrification and sulphur oxidation in coastal shrimp ponds is not reported much. In the present work, the Gram-negative, neutrophilic, facultatively lithoautotrophic bacterium Paracoccus saliphilus strain SPUM isolated from coastal shrimp ponds of Urran, Maharashtra, was studied for its efficiency of simultaneous heterotrophic nitrification–aerobic denitrification and sulphur oxidation processes. The maximum removal rate of nitrite and nitrate was 11.22 ± 0.31 and 14.17 ± 0.31 mg of NO3-N/l respectively after 24 h of incubation, while the sulphate-sulphur production observed was 190 ± 4.3 mg l−1 with a change in pH from 8.0 to 7.4 ± 0.08 after 12 days of incubation. The strain was characterized using universal 16S rRNA gene primers revealing high similarity (> 99%) with Paracoccus saliphilus belonging to α-proteobacteria. The isolate could express sulphate thiolesterase/sulphate thiohydrolase, soxB gene which is essential for sulphur oxidation. From all the results, it has been found that the strain SPUM could play a major role in simultaneous aerobic nitrification/denitrification and sulphur oxidation processes to overcome the toxicity of nitrogenous and sulphur-reducing compounds respectively in coastal aquaculture and wastewater systems.


Paracoccus saliphilus Nitrification/denitrification Sulphate thiolesterase/sulphate thiohydrolase Coastal shrimp ponds 



The authors are grateful to Director and Vice-Chancellor, ICAR-Central Institute of Fisheries Education, Mumbai, India, for providing support and necessary facilities to carry out this experiment. The first author would like to thank P. Priti, K. Radhika and K.Rateesh for their help at different stages of this research work.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical statement

This article does not contain any studies with animals performed by any of the authors.


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© Springer Nature Switzerland AG 2019

Authors and Affiliations

  1. 1.Division of Irrigation and Drainage EngineeringICAR-Central Soil Salinity InstituteKarnalIndia
  2. 2.Fisheries Resources, Harvest and Post Harvest DivisionICAR-Central Institute of Fisheries EducationMumbaiIndia
  3. 3.ICAR-Central Marine Fisheries InstituteKochiIndia
  4. 4.College of Fisheries ScienceBirsa Agricultural UniversityRanchiIndia
  5. 5.Division of Fish Nutrition, Biochemistry and PhysiologyICAR-Central Institute of Fisheries EducationMumbaiIndia
  6. 6.Fisheries Resource ManagementICAR-Directorate of Cold Water Fisheries ResearchBhimtalIndia
  7. 7.Fish PathologyICAR-Directorate of Cold Water Fisheries ResearchBhimtalIndia
  8. 8.Division of Crop ProductionICAR-Sugarcane Breeding InstituteCoimbatoreIndia

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