Streptococcosis in Oreochromis sp.: is feed-based biofilm vaccine of Streptococcus agalactiae effective?
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This study was carried out to determine the potential of a biofilm vaccine of Streptococcus agalactiae in protecting tilapia from streptococcocal infection. The tilapia were vaccinated orally using the biofilm vaccine with 1091010 CFU/g (FBV) incorporated in feed and killed whole-cell vaccine (FCV) of S. agalactiae also prepared in feed, and the unvaccinated control group (Cx) was fed with standard feed formulation. Throughout the study, serum, mucus and gut lavage fluid were collected to evaluate the antibody responses among the groups. The results showed that tilapia vaccinated with FBV exhibited significantly (p < 0.05) stronger immune response and high protection with the relative percentage survival (RPS) of more than 70% (85%) compared with the FCV group, which was only 56%. None of the tilapia in the Cx group survived (0%) post-challenge. Thus, the study revealed that the feed-based biofilm vaccine (FBV) significantly confers the immune protection against the high-dose challenge of S. agalactiae by secretion of the protective immunity in the mucosal and humoral in tilapia.
KeywordsBiofilm Streptococcus agalactiae Oreochromis sp. Oral vaccination
We would like to thank the farmer involved in this study and staff of Aquaculture Experimental Station, Faculty of Agriculture, UPM, Puchong, Selangor, for their excellent technical assistance.
This project was funded by the Ministry of Science, Technology and Innovation, Malaysia (MOSTI) under the vote no. 5450652.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
All applicable institutional guidelines for the care and use of animals were followed by the authors.
- Alcamo IE (1997) Environment and industrial microbiology. Fundamentals of microbiology, 5th edn. Addison Wesley Longman, Inc, California, p 766Google Scholar
- Azad IS, Shankar KM, Mohan CV (1997) Evaluation of biofilm of Aeromonas hydrophila for oral vaccination of carps. Diseases in Asian aquaculture III. Fish Health Section, Asian Fisheries Society, Manila, pp 181–186Google Scholar
- Davidson GA, Ellis AE, Secombes CJ (1993) Route of immunization influences the generation of antibody-secreting cells in the gut of rainbow trout (Oncorhynchus mykiss). Developmental and Comparative Immunology17, pp 373–376Google Scholar
- Kiyono H, Bienenstock J, McGhee JR, Ernst PB (1992) The mucosal immune system: features of inductive and effector sites to consider in mucosal immunization and vaccine development. Reg Immunol 4:54–62Google Scholar
- Klesius PH, Evans JJ, Shoemaker CA, Lim C (2006) A US perspective on selected biotechnological advancements in fish health. Aquac Health Int 5:10–12Google Scholar
- Liaghat M, Akhlaghi M, Hosseini A, Nematollahi A, Hosseini SM (2011) Humoral and non-specific immune responses in rainbow trout (Oncorhynchus mykiss) naturally exposed to and immunized with Streptococcus iniae. Int J Vet Res 5(4):218–224Google Scholar
- Noraini O, Sabri MY, Siti-Zahrah A (2013) Efficacy of spray administration of formalin-killed Streptococcus agalactiae in hybrid red tilapia. J Aquat Anim Health 25(2):142–148Google Scholar
- Nur-Nazifah M, Sabri MY, Siti-Zahrah A (2014) Development and efficacy of feed based recombinant vaccine encoding the cell wall surface anchor family protein of Streptococcus agalactiae against streptococcosis in Oreochromis sp. Fish Shellfish Immunol 37(1):193–200Google Scholar
- Plumb JA, Hanson LA (2011) Health maintenance and principal microbial diseases of cultured fishes. John Wiley & SonsGoogle Scholar
- Quentel C, Vigneulle M (1997) Antigen uptake and immune responses after oral vaccination. Dev Biol Stand 90:69–78Google Scholar
- Siti-Zahrah A, Misri S, Padilah B, Zulkafli R, Kua BC, Azila A R Rimatulhana. 2004. Pre-disposing factors associated with outbreak of streptococcal infection in floating cage-cultured red tilapia in reservoirs. Abstracts of the 7th Asian Fisheries Forum 04, The Triennial Meeting of The Asian Fisheries Society 30th Nov–4th Dec 2004, Penang, Malaysia. pp. 129.Google Scholar
- Soltani M, Jamshidi S, Sharifpour I (2005) Streptococcosis caused by Streptococcus iniae in farmed rainbow trout (Oncorhynchus mykiss) in Iran: biophysical characteristics and pathogenesis. Bull Eur Assoc Fish Pathol 25(3):95–106Google Scholar