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First use of a non-invasive technique for determination of sex hormones in the queen conch Lobatus gigas, Mollusca Gastropoda

  • Fabiola Chong Sánchez
  • Martha Enriquez Díaz
  • Eric Murillo Rodríguez
  • Dalila Aldana ArandaEmail author
Article
  • 25 Downloads

Abstract

Profiles were generated of the sex hormones estrogen, progesterone, and testosterone in the marine snail Lobatus gigas every 2 months for 1 year. A non-invasive technique involving feces collection was used. Hormones were extracted from the feces with 80% methanol. After filtering, hormone concentrations were measured by high-performance liquid chromatography and a UV detector. All three sex hormones were present in L. gigas feces, and their concentrations increased contemporaneously with the conch reproductive period. Males and females showed the highest concentration of testosterone in May (1.8 ± 0.3 and 2.1 ± 0.8 ng/ml, respectively). Both sexes presented a maximum estrogen concentration in May (1.2 ± 0.7 and 1.0 ± 0.3 ng/ml). Progesterone in females remained constant from March to July. Pearson correlation between estrogen and spawning activity were r = 0.66 (p = 0.03) and for testosterone (r = 0.5216) and progesterone (r = 0.437). This study constitutes the first use of this technique in this species. Results show that sex hormones may be controlling this species’ reproductive events, as occurs in other gastropods. The understanding of the L. gigas reproductive process, this is one of several steps that will allow in the future improve aquaculture systems and supporting conservation of wild populations.

Keywords

Non-invasive Queen conch Reproduction Sex hormones Strombus 

Abbreviations

GnRH

Gonadotropin-releasing hormone

HPLC

High-performance liquid chromatography

UV

Ultraviolet

Notes

Funding information

This work was supported by a National Council for Science and Technology project (Ciencia Básica-CONACyT No. 181329), and a scholarship granted to Fabiola Chong Sanchez (Conacyt, Mexico). English translation service by John Lindsay-Edwards was supported by FOMIX Doctorado FOMIX-YUC-2014-C17-247043. Authors acknowledge to the Xel-Ha park for providing the space that allowed the taking of samples to carry out this work.

Compliance with ethical standards

Declaration of interest

The authors declare no conflict of interest that could affect the impartiality of the research reported here.

Statement of informed consent/Human and animal rights and informed consent

All procedures performed in this study were in accordance with the ethical standards of the Internal Committee for the Care and Use of Laboratory Animals (Cicual) and accordance with the Official Mexican Standard NOM-062-ZOO-1999 and NOM-013-SAG / PESC-2016.

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Copyright information

© Springer Nature Switzerland AG 2019

Authors and Affiliations

  • Fabiola Chong Sánchez
    • 1
  • Martha Enriquez Díaz
    • 1
  • Eric Murillo Rodríguez
    • 2
  • Dalila Aldana Aranda
    • 1
    Email author
  1. 1.Unidad Mérida, Laboratorio de Biología y Cultivo de MoluscosCentro de Investigación y de Estudios Avanzados del Instituto Politécnico NacionalMéridaMexico
  2. 2.Laboratorio de Neurociencias Moleculares e Integrativas, Escuela de Medicina, División Ciencias de la SaludUniversidad Anahuac MayabMéridaMexico

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