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Microeukaryote community and the nutritional composition of the biofloc during Nile tilapia culture in water-reusing biofloc systems

  • Alfredo Gallardo-Collí
  • Carlos Iván Pérez-Rostro
  • Martha Patricia Hernández-Vergara
  • Ignacio Alejandro Pérez-Legaspi
Article
  • 19 Downloads

Abstract

A 25-week experiment was conducted to investigate the changes in the microeukaryote community (MEC) and nutritional composition of the biofloc during Nile tilapia culture in water-reusing biofloc systems. The study evaluated two treatments: tilapia culture in biofloc (TB) and tilapia culture in biofloc with reuse water (RWTB). Each of the treatments was evaluated using a main culture tank (3 m3) and three experimental tanks (0.2 m3). The biofloc samples were revised with an inverted microscope and the groups of microorganisms and genera of the MEC were recorded. Total abundance of microorganisms (TAM), number of accumulated genera, ecological indices and water quality were evaluated. The MEC of both treatments consisted of microalgae, ciliates, flagellates, amoebas, rotifers, nematodes and annelids; the number of accumulated genera and TAM in RWTB (74 genera, 161.52 ± 80.60 org mL−1) was significantly greater than in TB (63 genera, 139.54 ± 81.39 org mL−1). The TAM in TB and RWTB varied in relation to the temperature, sedimentable solids, NH4-N and NH3-N. The dominance index observed in the RWTB group (0.59) was statistically greater than in the TB group (0.57) during the first 2 weeks of the study. The nutritional composition of the biofloc varied with time; at the end of the study, an increase in the percentage of protein (47%) and a reduction in the percentage of lipids (2%) was observed when compared with values recorded at the beginning of the study. The results suggest that both the richness and TAM of the MEC increase in water-reusing biofloc systems and that the biochemical constitution of the microorganisms which constitute the MEC affects the proximal composition of the biofloc.

Keywords

Biofloc technology Ecological index Eukaryotic microorganism Microbial community Reuse water Single cell protein 

Abbreviations

MEC

Microeukaryote community

TB

Tilapia culture in biofloc

RWTB

Tilapia culture in biofloc with reuse water

TAM

Total abundance of microorganisms

BFT

Biofloc technology

SS

Settleable solids

T

Temperature

DO

Dissolved oxygen

TDS

Total dissolved salts

GH

General hardness

KH

Carbonate hardness

NFE

Nitrogen-free extract

PC1

Principal component 1

PC2

Principal component 2

Notes

Acknowledgements

The authors would also like to thank two anonymous reviewers for their valuable comments on an earlier version of this manuscript.

Funding information

A.G.C. is the grant for posgraduate support by the Consejo Nacional de Ciencia y Tecnología de México (CONACYT, grant No. 229713).

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Copyright information

© Springer Nature Switzerland AG 2019

Authors and Affiliations

  1. 1.Laboratorio de Mejoramiento Genético y Producción AcuícolaInstituto Tecnológico de Boca del RíoVeracruzMexico

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