Bacterial complexes of Bacillus subtilis and Pseudomonas stutzeri alter the microbial composition in grass carp water
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The current study was designed to evaluate the effects of bacterial complexes (BC, a combination of Bacillus subtilis SC02 and Pseudomonas stutzeri F1M) on nitrogen removal and microbial composition in grass carp culture water. The results of the study showed that the addition of BC could significantly decrease the level of harmful nitrogen compounds and improve the water quality in grass carp culture water over an extended period of time. The 454-pyrosequencing results showed that BC could significantly increase (p < 0.05) the abundance of the genera Hydrogenophaga and Cloacibacterium on the 6th day and 15th day, respectively. LefSe analysis (LDA Score [log10] > 4) indicated that BC enriched in the taxa Firmicutes, Hydrogenophaga, and Pseudomonas, while the CK was enriched in the genus Rhodobacter on the 6th day. On the 15th day, the CK microbiome was characterized by a preponderance of Betaproteobacteria in the water, but no biomarker was identified in the BC group; therefore, the addition of a combination of Bacillus subtilis SC02 and Pseudomonas stutzeri F1M can improve water quality and change the microbiota in grass carp water.
KeywordsAquaculture water Nitrogen removal Microbial diversity Grass carp
This study was supported by the Zhejiang Provincial Key Project of Science and Technology Research (No. 2015C02054) and National Basic Research Program, P.R. China (973 Program) (No. 2009CB118705).
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflicts of interest.
This study involving the use of animals was conducted in accordance with the guidelines of the Animal Ethics Committee of the Institute of Animal Nutrition and Feed Science, College of Animal Science, Zhejiang University.
- Boyd CE (1995) Chemistry and efficacy of amendments used to treat water and soil quality imbalances in shrimp ponds. In: Proceedings of the special session on shrimp farming. The World Aquaculture Society, Baton Rouge, pp 183–189Google Scholar
- Caporaso JG, Kuczynski J, Stombaugh J, Bittinger K, Bushman FD, Costello EK, Fierer N, Pena AG, Goodrich JK, Gordon JI, Huttley GA, Kelley ST, Knights D, Koenig JE, Ley RE, Lozupone CA, McDonald D, Muegge BD, Pirrung M, Reeder J, Sevinsky JR, Turnbaugh PJ, Walters WA, Widmann J, Yatsunenko T, Zaneveld J, Knight R (2010) QIIME allows analysis of high-throughput community sequencing data. Nat Methods 7:335–336CrossRefGoogle Scholar
- EPBC (2002) Methods for monitor and analysis of water and wastewater (4rd ed), China Environmental Science Press,BeijingGoogle Scholar
- Gao F, Liao S, Liu S, Bai H, Wang A, Ye J (2018) The combination use of Candida tropicalis HH8 and Pseudomonas stutzeri LZX301 on nitrogen removal, biofloc formation and microbial communities in aquaculture. Aquaculture 500:50–56. https://doi.org/10.1016/j.aquaculture.2018.09.041 CrossRefGoogle Scholar
- Gray ND, Sherry A, Grant RJ, Rowan AK, Hubert CRJ, Callbeck CM, Aitken CM, Jones DM, Adams JJ, Larter SR, Head IM (2011) The quantitative significance of Syntrophaceae and syntrophic partnerships in methanogenic degradation of crude oil alkanes. Environ Microbiol 13(11):2957–2975CrossRefGoogle Scholar
- Haas BJ, Gevers D, Earl AM, Feldgarden M, Ward DV, Giannoukos G, Ciulla D, Tabbaa D, Highlander SK, Sodergren E, Methe B, DeSantis TZ, Petrosino JF, Knight R, Birren BW, Consortium HM (2011) Chimeric 16S rRNA sequence formation and detection in sanger and 454-pyrosequenced PCR amplicons. Genome Res 21(3):494–504CrossRefGoogle Scholar
- Knowles R (1982) Denitrification. Microbiol Rev 46(1):43–70Google Scholar
- Subasinghe RP, Barg U, Tacon A (2000) Chemicals in Asian aquaculture: need, usage, issues and challenges[C]//Use of chemicals in aquaculture in Asia: Proceedings of the meeting on the use of chemicals in aquaculture in Asia 20–22 May 1996, Tigbauan, Iloilo, Philippines. SEAFDEC Aquaculture Department:1–5Google Scholar
- Tchobanoglous G, Metcalf and Eddy Inc., (1991) Wastewater engineering treatment, disposal and reuse. McGraw-Hill series in water resources and environmental engineering 73(1): 50–51Google Scholar
- Zheng JJ, Zhang XP, Hu C, Li WF (2013) Effect of Pseudomonas stutzeri F1M on water quality in grass carp culture. Fishery Modernization 40(3):5–9Google Scholar