Advertisement

Aquaculture International

, Volume 27, Issue 1, pp 303–312 | Cite as

Bacterial complexes of Bacillus subtilis and Pseudomonas stutzeri alter the microbial composition in grass carp water

  • Xiaoping Zhang
  • Yang Wang
  • Zheke Zhong
  • Qingjun Shao
  • Yibing Wang
  • Weifen Li
Article
  • 35 Downloads

Abstract

The current study was designed to evaluate the effects of bacterial complexes (BC, a combination of Bacillus subtilis SC02 and Pseudomonas stutzeri F1M) on nitrogen removal and microbial composition in grass carp culture water. The results of the study showed that the addition of BC could significantly decrease the level of harmful nitrogen compounds and improve the water quality in grass carp culture water over an extended period of time. The 454-pyrosequencing results showed that BC could significantly increase (p < 0.05) the abundance of the genera Hydrogenophaga and Cloacibacterium on the 6th day and 15th day, respectively. LefSe analysis (LDA Score [log10] > 4) indicated that BC enriched in the taxa Firmicutes, Hydrogenophaga, and Pseudomonas, while the CK was enriched in the genus Rhodobacter on the 6th day. On the 15th day, the CK microbiome was characterized by a preponderance of Betaproteobacteria in the water, but no biomarker was identified in the BC group; therefore, the addition of a combination of Bacillus subtilis SC02 and Pseudomonas stutzeri F1M can improve water quality and change the microbiota in grass carp water.

Keywords

Aquaculture water Nitrogen removal Microbial diversity Grass carp 

Notes

Funding information

This study was supported by the Zhejiang Provincial Key Project of Science and Technology Research (No. 2015C02054) and National Basic Research Program, P.R. China (973 Program) (No. 2009CB118705).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflicts of interest.

Ethical statement

This study involving the use of animals was conducted in accordance with the guidelines of the Animal Ethics Committee of the Institute of Animal Nutrition and Feed Science, College of Animal Science, Zhejiang University.

Supplementary material

10499_2018_325_MOESM1_ESM.doc (4.6 mb)
ESM 1 (DOC 4722 kb)

References

  1. Allen TD, Lawson PA, Collins MD, Falsen E, Tanner RS (2006) Cloacibacterium normanense gen. nov., sp. nov., a novel bacterium in the family Flavobacteriaceae isolated from municipal wastewater. Int J Syst Evol Microbiol 56(6):1311–1316CrossRefGoogle Scholar
  2. Arndt D, Xia JG, Liu YF, Zhou Y, Guo AC, Cruz JA, Sinelnikov I, Budwill K, Nesbo CL, Wishart DS (2012) METAGENassist: a comprehensive web server for comparative metagenomics. Nucleic Acids Res 40(W1):W88–W95CrossRefGoogle Scholar
  3. Bondad-Reantaso MG, Subasinghe RP, Arthur JR, Ogawa K, Chinabut S, Adlard R, Tan Z, Shariff M (2005) Disease and health management in Asian aquaculture. Vet Parasitol 132(3–4):249–272CrossRefGoogle Scholar
  4. Boyd CE (1995) Chemistry and efficacy of amendments used to treat water and soil quality imbalances in shrimp ponds. In: Proceedings of the special session on shrimp farming. The World Aquaculture Society, Baton Rouge, pp 183–189Google Scholar
  5. Caporaso JG, Kuczynski J, Stombaugh J, Bittinger K, Bushman FD, Costello EK, Fierer N, Pena AG, Goodrich JK, Gordon JI, Huttley GA, Kelley ST, Knights D, Koenig JE, Ley RE, Lozupone CA, McDonald D, Muegge BD, Pirrung M, Reeder J, Sevinsky JR, Turnbaugh PJ, Walters WA, Widmann J, Yatsunenko T, Zaneveld J, Knight R (2010) QIIME allows analysis of high-throughput community sequencing data. Nat Methods 7:335–336CrossRefGoogle Scholar
  6. Cole JR, Wang Q, Fish JA, Chai BL, McGarrell DM, Sun YN, Brown CT, Porras-Alfaro A, Kuske CR, Tiedje JM (2014) Ribosomal database project: data and tools for high throughput rRNA analysis. Nucleic Acids Res 42(D1):D633–D642CrossRefGoogle Scholar
  7. DeSantis TZ, Hugenholtz P, Larsen N, Rojas M, Brodie EL, Keller K, Huber T, Dalevi D, Hu P, Andersen GL (2006) Greengenes, a chimera-checked 16S rRNA gene database and workbench compatible with ARB. Appl Environ Microbiol 72(7):5069–5072CrossRefGoogle Scholar
  8. Edgar RC (2010) Search and clustering orders of magnitude faster than BLAST. Bioinformatics 26(19):2460–2461CrossRefGoogle Scholar
  9. EPBC (2002) Methods for monitor and analysis of water and wastewater (4rd ed), China Environmental Science Press,BeijingGoogle Scholar
  10. Gao F, Liao S, Liu S, Bai H, Wang A, Ye J (2018) The combination use of Candida tropicalis HH8 and Pseudomonas stutzeri LZX301 on nitrogen removal, biofloc formation and microbial communities in aquaculture. Aquaculture 500:50–56.  https://doi.org/10.1016/j.aquaculture.2018.09.041 CrossRefGoogle Scholar
  11. Gray ND, Sherry A, Grant RJ, Rowan AK, Hubert CRJ, Callbeck CM, Aitken CM, Jones DM, Adams JJ, Larter SR, Head IM (2011) The quantitative significance of Syntrophaceae and syntrophic partnerships in methanogenic degradation of crude oil alkanes. Environ Microbiol 13(11):2957–2975CrossRefGoogle Scholar
  12. Haas BJ, Gevers D, Earl AM, Feldgarden M, Ward DV, Giannoukos G, Ciulla D, Tabbaa D, Highlander SK, Sodergren E, Methe B, DeSantis TZ, Petrosino JF, Knight R, Birren BW, Consortium HM (2011) Chimeric 16S rRNA sequence formation and detection in sanger and 454-pyrosequenced PCR amplicons. Genome Res 21(3):494–504CrossRefGoogle Scholar
  13. Hagi T, Tanaka D, Iwamura Y, Hoshino T (2004) Diversity and seasonal changes in lactic acid bacteria in the intestinal tract of cultured freshwater fish. Aquaculture 234(1):335–346CrossRefGoogle Scholar
  14. Hu M, Wang XH, Wen XH, Xia Y (2012) Microbial community structures in different wastewater treatment plants as revealed by 454-pyrosequencing analysis. Bioresour Technol 117:72–79CrossRefGoogle Scholar
  15. Kleinsteuber S, Schleinitz KM, Vogt C (2012) Key players and team play: anaerobic microbial communities in hydrocarbon-contaminated aquifers. Appl Microbiol Biotechnol 94(4):851–873CrossRefGoogle Scholar
  16. Knowles R (1982) Denitrification. Microbiol Rev 46(1):43–70Google Scholar
  17. Kormas KA, Meziti A, Mente E, Frentzos A (2014) Dietary differences are reflected on the gut prokaryotic community structure of wild and commercially reared sea bream (Sparus aurata). MicrobiologyOpen 3(5):718–728CrossRefGoogle Scholar
  18. Li XP, Li JR, Wang YB, Fu LL, Fu YY, Li BQ, Jiao BH (2011) Aquaculture industry in China: current state, challenges, and outlook. Rev Fish Sci 19(3):187–200CrossRefGoogle Scholar
  19. Liang Q, Zhang XP, Lee KH, Wang YB, Yu K, Shen WY, Fu LQ, Shu MA, Li WF (2015) Nitrogen removal and water microbiota in grass carp culture following supplementation with Bacillus licheniformis BSK-4. World J Microbiol Biotechnol 31(11):1711–1718CrossRefGoogle Scholar
  20. Moriarty DJW (1997) The role of microorganisms in aquaculture ponds. Aquaculture 151(1):333–349CrossRefGoogle Scholar
  21. Oerther DB, de los Reyes FL, de los Reyes MF, Raskin L (2001) Quantifying filamentous microorganisms in activated sludge before, during, and after an incident of foaming by oligonucleotide probe hybridizations and antibody staining. Water Res 35(14):3325–3336CrossRefGoogle Scholar
  22. Segata N, Izard J, Waldron L, Gevers D, Miropolsky L, Garrett WS, Huttenhower C (2011) Metagenomic biomarker discovery and explanation. Genome Biol 12:R60CrossRefGoogle Scholar
  23. Semova I, Carten Juliana D, Stombaugh J, Mackey Lantz C, Knight R, Farber Steven A, Rawls John F (2012) Microbiota regulate intestinal absorption and metabolism of fatty acids in the zebrafish. Cell Host Microbe 12(3):277–288CrossRefGoogle Scholar
  24. Spanggaard B, Huber I, Nielsen J, Nielsen T, Appel KF, Gram L (2000) The microflora of rainbow trout intestine: a comparison of traditional and molecular identification. Aquaculture 182:1): 1–1):15CrossRefGoogle Scholar
  25. Subasinghe RP, Barg U, Tacon A (2000) Chemicals in Asian aquaculture: need, usage, issues and challenges[C]//Use of chemicals in aquaculture in Asia: Proceedings of the meeting on the use of chemicals in aquaculture in Asia 20–22 May 1996, Tigbauan, Iloilo, Philippines. SEAFDEC Aquaculture Department:1–5Google Scholar
  26. Tchobanoglous G, Metcalf and Eddy Inc., (1991) Wastewater engineering treatment, disposal and reuse. McGraw-Hill series in water resources and environmental engineering 73(1): 50–51Google Scholar
  27. Wang YB, Xu ZR, Xia MS (2005) The effectiveness of commercial probiotics in northern white shrimp Penaeus vannamei ponds. Fish Sci 71(5):1036–1041CrossRefGoogle Scholar
  28. Wu YH, Li TL, Yang LZ (2012) Mechanisms of removing pollutants from aqueous solutions by microorganisms and their aggregates: a review. Bioresour Technol 107:10–18CrossRefGoogle Scholar
  29. Xing W, Li J, Li P, Wang C, Cao Y, Li D, Yang Y, Zhou J, Zuo J (2018) Effects of residual organics in municipal wastewater on hydrogenotrophic denitrifying microbial communities. J Environ Sci 65:262–270CrossRefGoogle Scholar
  30. Yong Y-C, Wu X-Y, Sun J-Z, Cao Y-X, Song H (2015) Engineering quorum sensing signaling of Pseudomonas for enhanced wastewater treatment and electricity harvest: a review. Chemosphere 140:18–25CrossRefGoogle Scholar
  31. Zhang X, Fu L, Deng B, Liang Q, Zheng J, Sun J, Zhu H, Peng L, Wang Y, Wenying S, Li W (2013) Bacillus subtilis SC02 supplementation causes alterations of the microbial diversity in grass carp water. World J Microbiol Biotechnol 29(9):1645–1653CrossRefGoogle Scholar
  32. Zhang X, Shu M, Wang Y, Fu L, Li W, Deng B, Liang Q, Shen W (2014) Effect of photosynthetic bacteria on water quality and microbiota in grass carp culture. World J Microbiol Biotechnol 30(9):2523–2531CrossRefGoogle Scholar
  33. Zheng JJ, Zhang XP, Hu C, Li WF (2013) Effect of Pseudomonas stutzeri F1M on water quality in grass carp culture. Fishery Modernization 40(3):5–9Google Scholar

Copyright information

© Springer Nature Switzerland AG 2018

Authors and Affiliations

  • Xiaoping Zhang
    • 1
  • Yang Wang
    • 2
  • Zheke Zhong
    • 1
  • Qingjun Shao
    • 2
  • Yibing Wang
    • 2
  • Weifen Li
    • 2
    • 3
  1. 1.China National Bamboo Research Center, Key Laboratory of Resources and Utilization of Bamboo of State Forestry AdministrationHangzhouPeople’s Republic of China
  2. 2.College of Animal SciencesZhejiang UniversityHangzhouPeople’s Republic of China
  3. 3.Institute of Feed Sciences, College of Animal SciencesZhejiang UniversityHangzhouPeople’s Republic of China

Personalised recommendations