Alcalase enzyme treatment affects egg incubation and larval quality in pikeperch (Sander lucioperca)
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Although egg de-adhesion has been the subject of research in pikeperch of late, the possible effect of this technological procedure on larval viability under intensive rearing conditions has not yet been evaluated. The aim of the present study was to evaluate the effect of the Alcalase enzyme on egg incubation and larviculture success compared to a commonly used procedure with milk and kaolin clay suspension. Preliminary research was conducted in order to find the minimal exposure time of eggs in Alcalase enzyme solution for the total elimination of adhesiveness. Further on, stripped eggs from three females were divided into two equal portions, and each portion was treated according to the abovementioned procedures. Efficiency of the procedures was evaluated and compared through egg incubation and larviculture. Alcalase-treated eggs exhibited significantly shorter incubation time (121 ± 12 h vs. 157 ± 10 h), hatching period (16 ± 7 h vs. 48 ± 21 h) and lower embryo survival (82.5 ± 2.4% vs. 87.7 ± 1.4%) with a significantly higher hatching rate (98.5 ± 1.0% vs. 72.0 ± 35.3 %). The larviculture yielded significantly lower production efficacy in eggs treated with Alcalase manifested as the share of larvae with an inflated swim bladder in the total number of stocked eggs (5.8 ± 2.4%) compared to larvae hatched in eggs treated with milk and kaolin (20.1 ± 11.9%). The Alcalase enzyme treatment reduced the incubation time and diminished the larval performance; therefore, its application in eggs of pikeperch should be reconsidered.
KeywordsAlcalase Milk Kaolin Egg de-adhesion Hatching Larviculture
Alcalase enzyme egg de-adhesion treatment
final oocyte maturation
human chorionic gonadotropin
milk plus kaolin egg de-adhesion treatment
recirculation aquaculture system
This work was supported by the European Regional and Development Fund and the Government of Hungary within the project GINOP-2.3.2-15-2016-00025.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
All applicable international, national and/or institutional guidelines for the care and use of animals were followed by the authors.
- Al Hazzaa R, Hussein A (2003) Stickiness elimination of Himri Barbel (Barbus lutes, Heckel) eggs. TrFJAS 3:47–50Google Scholar
- Becker CD, Neitzel DA, Abernethy CS (1983) Effects of dewatering on Chinook salmon redds: tolerance of four development phases to one-time dewatering. N Am J Fish Manag 3:373–382. https://doi.org/10.1577/1548-8659(1983)3<373:EODOCS>2.0.CO;2 CrossRefGoogle Scholar
- Ben Khemis IB, Hamza N, Messaoud NB, Rached SB, M’Hetli M (2014) Comparative study of pikeperch Sander lucioperca (Percidae; Linnaeus, 1758) eggs and larvae from wild females or from captive females fed chopped marine fish. Fish Physiol Biochem 40:375–384. https://doi.org/10.1007/s10695-013-9850-2 CrossRefPubMedGoogle Scholar
- Dabrowski K, Czesny S, Kolkovski S, Lynch WE Jr, Bajer P, Culver DA (2000) Intensive culture of walleye larvae produced out of season and during regular season spawning. N Am J Aquac 62:219–224. https://doi.org/10.1577/1548-8454(2000)062<0219:ICOWLP>2.3.CO;2 CrossRefGoogle Scholar
- Hermelink B, Wuertz S, Rennert B, Kloas W, Schulz C (2013) Temperature control of pikeperch (Sander lucioperca) maturation in recirculating aquaculture systems -induction of puberty and course of gametogenesis. Aquaculture 400:36–45. https://doi.org/10.1016/j.aquaculture.2013.02.026 CrossRefGoogle Scholar
- Hermelink B, Kleiner W, Schulz C, Kloas W, Wuertz S (2016) Photo-thermal manipulation for the reproductive management of pikeperch Sander lucioperca. Aquac Int 25: 1–20. https://doi.org/10.1007/s10499-016-0009-x
- Hilge V, Steffens W (1996) Aquaculture of fry and fingerling of pike-perch (Stizostedion lucioperca) - a short review. J Appl Ichthyol 12:167–170. https://doi.org/10.1111/j.1439-0426.1996.tb00083.x CrossRefGoogle Scholar
- Krise WF (1988) Optimum protease exposure time for removing adhesiveness of walleye eggs. Prog fish-cult 50: 126–127.Google Scholar
- Krise WF, Bulkowski-Cummings L, Shellman DA, Kraus KA, Gould RW (1986) Increased walleye egg hatch and larval survival after protease treatment of eggs. Prog fish-cult 48:95–100. https://doi.org/10.1577/1548-8640(1988)050<0126:OPETFR>2.3.CO;2 CrossRefGoogle Scholar
- Kucharczyk D, Kestemont P, Mamcarz A (2007) Artificial reproduction of pikeperch. Mercurius, Olsztyn 80 pGoogle Scholar
- Linhart O, Rodina M, Gela D, Flajšhans M, Kocour M (2003) Enzyme treatment for elimination of egg stickiness in tench (Tinca tinca L.), European catfish (Silurus glanis L.) and common carp (Cyprinus carpio L.). Fish Physiol Biochem 28:507–508. https://doi.org/10.1023/B:FISH.0000 CrossRefGoogle Scholar
- Ljubobratović U, Balogh E, Lengyel S, Kovacs Gy, Adorjan A, Janurik E, Csengeri I, Ronyai A (2014) Improved techniques for egg de-adhesion in pikeperch, Sander lucioperca. Paper presented at the 28th Halászati Tudományos Tanácskozás. Szarvas, Hungary, 28–29 July Available at http://hakinapok.haki.hu/tartalom/2014/Poszter/11_Ljubobratovic%20et%20al%20%202,014.pdf
- Ljubobratović U, Csengeri I, Kucska B, Balogh E, Lengyel S, Kovács G, Adorjan A, Feledi T, Janurik E, Rónyai A (2017a) Comparison of the procedures for adhesiveness removal in pikeperch (Sander lucioperca) eggs with special emphasis on the effect of tannic acid. TrFJAS 17:461–469. https://doi.org/10.4194/1303-2712-v17_3_02 CrossRefGoogle Scholar
- Ljubobratović U, Péter G, Horváth Z, Żarski D, Ristović T, Percze V, Zs S, Lengyel S, Rónyai A (2017b) Reproductive performance of indoor-reared pikeperch (Sander lucioperca) females after wintering in outdoor earthen ponds. Aquac Res 48:4851–4863. https://doi.org/10.1111/are.13305 CrossRefGoogle Scholar
- Ljubobratović U, Péter G, Horváth Z, Demény F, Rónyai A (2018) HANDBOOK – State-of-the-art in zander (Sander lucioperca) artificial reproduction at NAIK HAKI institute. In: Poleksić V, Marković Z (eds) Proceedings of the VIIIth International conference “Water and Fish”. University of Belgrade - Faculty of Agriculture, Belgrade-Zemun, pp 500–517Google Scholar
- Mani-Ponset L, Diaz JP, Schlumberger O, Connes R (1994) Development of yolk complex, liver and anterior intestine in pike perch larvae, Stizostedion lucioperca (Percidae), according to the first diet during rearing. Aquat Living Resour 7:191–202. https://doi.org/10.1051/alr:1994021 CrossRefGoogle Scholar
- Nahm FS (2016) Nonparametric statistical tests for the continuous data: the basic concept and the practical use. Korean J Anesthesiol 69: 8–14. https://doi.org/10.4097/kjae.2016.69.1.8.
- Rasband WS (1997–2011) ImageJ. U.S. National Institutes of Health, Bethesda, Maryland, USAGoogle Scholar
- Rónyai A, Gál D (2008) Deadhesion of the eggs of pikeperch (Sander lucioperca L.). In: Fontaine P, Kestemont P, Teletchea F, Wang N (eds) Percid fish culture, from research to production. Presses Universitaires de Namur, Namur, p 150Google Scholar
- Ruuhijärvi M, Virtanen E, Salminen J, Muyunda M (1991) The growth and survival of pike-perch (Stizostedion lucioperca L.), larvae fed formulated feeds. In: Lavens P, Sorgeloos P, Jespers E, Ollevier F (eds) LARVI‘91 – Fish Crustacean Larvicult Symp. European Aquaculture Society, Gent, Belgium, pp 154–156Google Scholar
- Steenfeldt S, Fontaine P, Overton JL, Policar T, Toner D, Falahatkar B, Horváth Á, Ben Khemis I, Hamza N, Mhetli M (2015) Current status of Eurasian percid fishes aquaculture. In: Kestemont P, Dabrowski K, Summerfelt RC (eds) Biology and culture of percid fishes. Springer, Berlin Heidelberg New York, pp 817–841CrossRefGoogle Scholar
- Summerfelt RC, Johnson JA, Clouse CP (2011) Culture of walleye, sauger, and hybrid walleye. In: Barton B (ed) Biology, management, and culture of walleye, sauger, and hybrid walleye American Fisheries Society Special Publication. Bethesda, Maryland, pp 451–570Google Scholar
- Zakęś Z, Demska-Zakęś K (2005) Artificial spawning of pikeperch (Sander lucioperca (L.)) stimulated with human chorionic gonadotropin (hCG) and mammalian GnRH analogue with a dopamine inhibitor. Arch Pol Fish 13: 63–75.Google Scholar
- Zakęś Z, Demska- Zakęś K, Roszuk K, Kowalska A (2006b) Odklejanie ikry sandacza (Sander lucioperca) przy użyciu taniny i proteazy. In: Zakes Z, Demska-Zakes K, Wolnicki J (eds) Rozród, podchów, profilaktyka ryb karpiowatych i innych gatunków – Wydawnictw IRS, Olsztyn, pp 239–249 (in Polish).Google Scholar
- Żarski D, Palińska K, Targońska K, Bokor Z, Kotrik L, Krejszeff S, Kupren K, Horváth A, Urbányi B, Kucharczyk D (2011) Oocyte quality indicators in Eurasian perch, Perca fluviatilis L., during reproduction under controlled conditions. Aquaculture 313:84–91. https://doi.org/10.1016/j.aquaculture.2011.01.032 CrossRefGoogle Scholar
- Żarski D, Krejszeff S, Kucharzyk D, Palińska K, Targońska K, Kupren K, Fontaine P, Kestemont P (2015) The application of tannic acid to the elimination of egg stickiness at varied moments of the egg swelling process in pikeperch, Sander lucioperca (L.). Aquac Res 46:324–334. https://doi.org/10.1111/are.12183 CrossRefGoogle Scholar