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Aquaculture International

, Volume 27, Issue 1, pp 209–225 | Cite as

Transcriptional response of immune-related genes in Litopenaeus vannamei post-larvae cultured in recirculating aquaculture systems with and without biofloc

  • Jorge Soto-Alcalá
  • Píndaro Álvarez-RuizEmail author
  • J. M. Audelo-Naranjo
  • H. M. Esparza-Leal
  • I. E. Luis-Villaseñor
  • J. A. Estrada-Godínez
  • A. Luna-González
  • C. Gámez-Jiménez
  • G. Diarte-Plata
Article
  • 44 Downloads

Abstract

In the present study, quantitative real-time RT-PCR was used to monitor the transcriptional responses of seven key genes related to some innate immune pathways in shrimp postlarvae after being placed in a recirculating aquaculture system (RAS) with or without biofloc (BF or no-BF). Each system consisted of the main tank with 400 L of seawater and six glass aquariums (50 L each). Besides, the nucleotide sequences of myosin light chain (LvMyo) from Litopenaeus vannamei related to the phagocytosis pathway were described. The sequence analysis indicated that LvMyo is a conserved protein among crustaceans and is present in other arthropods. The transcriptional response to the treatments showed several expression patterns. The prophenoloxidase gene was up-regulated in both systems (P < 0.05) and was higher in BF than in no-BF (P < 0.01). Phagocytosis-related genes depicted differential expressions. LvMyo, Ras-associated binding 6, and Ras-related nuclear protein expressions were higher in BF than in no-BF (P < 0.05). Regarding antioxidant genes, glutathione peroxidase was up-regulated only in BF (P < 0.05). Superoxide dismutase expression was lower in BF at 12 h (P < 0.05), but higher at 24 h (P < 0.05). These findings suggest that biofloc modulates the transcription of genes related to the immune response in shrimp as an early response or at the mid-term. Besides, the biological filter in a RAS without biofloc seems to be able to maintain a bacterial population that promotes a lower but similar response to that induced in the biofloc system.

Keywords

Biofloc Gene expression Glutathione peroxidase Heterotrophic culture Phagocytosis Prophenoloxidase RAS Superoxide dismutase Shrimp immune system 

Abbreviations

RAS

Recirculating aquaculture system

BF

Treatment with biofloc

no-BF

Treatment without biofloc

LvMyo

Litopenaeus vannamei myosine light chain

ProPO

Prophenoloxidase

Rab6

Ras-associated binding 6

Ran

Ras-related nuclear protein

Gpx

Glutathione peroxidase

SOD

Superoxide dismutase

PAP

Phagocytosis-activating protein

PL

Postlarvae

Notes

Acknowledgements

We thank CONACyT for the scholarship (166929) to Jorge Soto Alcalá.

Funding information

This study was funded by the Instituto Politécnico Nacional (SIP20151682 and SIP20160089).

Compliance with ethical standards

This study complies with the Mexican official Standard NOM-0062-ZOO-1999, technical specifications for the production, care and use of laboratory animals.

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer Nature Switzerland AG 2018

Authors and Affiliations

  • Jorge Soto-Alcalá
    • 1
    • 2
  • Píndaro Álvarez-Ruiz
    • 1
    Email author return OK on get
  • J. M. Audelo-Naranjo
    • 2
  • H. M. Esparza-Leal
    • 1
  • I. E. Luis-Villaseñor
    • 2
  • J. A. Estrada-Godínez
    • 2
  • A. Luna-González
    • 1
  • C. Gámez-Jiménez
    • 1
  • G. Diarte-Plata
    • 1
  1. 1.Instituto Politécnico NacionalCentro Interdisciplinario de Investigación para el Desarrollo Integral Regional-Unidad SinaloaGuasaveMexico
  2. 2.Universidad Autónoma de SinaloaFacultad de Ciencias del MarMazatlánMexico

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