pp 1–11 | Cite as

Genetic and epigenetic analysis of the BAX and BCL2 in the placenta of pregnant women complicated by preeclampsia

  • Abbas Mohammadpour-Gharehbagh
  • Danial jahantigh
  • Moein Eskandari
  • Mahdiyeh Harati Sadegh
  • Mohammad Hadi Nematollahi
  • Mahnaz Rezaei
  • Ava Rasouli
  • Fatemeh Eskandari
  • Milad Zadi Heydarabad
  • Batool Teimoori
  • Saeedeh SalimiEmail author


The current study examined the effects of BAX and BCL2 polymorphisms and methylation as well as mRNA expression on susceptibility to PE. After delivery, the placentas were collected from 92 women with PE, as well as 106 normotensive pregnant women. The BAX rs4645878 and BCL2 rs2279115 polymorphisms were genotyped by the PCR-RFLP method. Methylation-specific PCR (MSP) was used for analysis of promoter methylation. mRNA expression was assayed by Quantitative RT-PCR. In addition, in silico analysis was performed by bioinformatics tools. There was no relationship between PE and placental BAX rs4645878 and BCL2 rs2279115 polymorphisms. The groups were not significantly different regarding the promoter methylation of BAX gene. Nonetheless, the MM status of BCL2 promoter had a significantly higher frequency in the PE group and was associated with 2.7-fold higher risk of PE (OR = 2.7, 95% CI = 1.3–5.6; P = 0.01). The relative mRNA expression of BCL2 was decreased in the placentas of PE women (P < 0.0001). The expression of BAX gene was not significantly different between the two groups. There was no association between placental BAX rs4645878 and BCL2 rs2279115 polymorphisms and mRNA expression levels. In silico analysis indicated that BAX rs4645878 and BCL2 rs2279115 polymorphisms were located in the core recognition site of different transcription factors and these substitutions of wild allele resulted in the loss and/ or change of these binding sites and subsequently may alter BCL2 and BAX expression. This study showed that the BAX and BCL2 polymorphisms and BAX promoter methylation were not associated with PE risk. The BCL2 promoter methylation was associated with lower BCL2 expression and higher PE susceptibility.


Apoptosis Expression Methylation Preeclampsia Polymorphism 



The study was supported by the Research Deputy in Zahedan University of Medical Sciences. We wish to acknowledge all the participants (patients and controls) in this research.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical Approval

The Ethics Committee of Zahedan University of Medical Science approved study protocol (IR.zaums.REC.1396.44).


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Abbas Mohammadpour-Gharehbagh
    • 1
    • 2
  • Danial jahantigh
    • 3
  • Moein Eskandari
    • 4
  • Mahdiyeh Harati Sadegh
    • 5
    • 6
  • Mohammad Hadi Nematollahi
    • 7
  • Mahnaz Rezaei
    • 1
    • 2
  • Ava Rasouli
    • 4
  • Fatemeh Eskandari
    • 1
    • 2
  • Milad Zadi Heydarabad
    • 8
  • Batool Teimoori
    • 9
  • Saeedeh Salimi
    • 1
    • 2
    • 10
    Email author
  1. 1.Cellular and Molecular Research CenterZahedan University of Medical SciencesZahedanIran
  2. 2.Department of Clinical Biochemistry, School of MedicineZahedan University of Medical SciencesZahedanIran
  3. 3.Department of Biology, Faculty of ScienceUniversity of Sistan and BaluchestanZahedanIran
  4. 4.Department of Laboratory Sciences, School of Paramedical SciencesZahedan University of Medical SciencesZahedanIran
  5. 5.Department of Genetics, Fars Science and Research BranchIslamic Azad UniversityMarvdashtIran
  6. 6.Department of Genetics, Marvdasht BranchIslamic Azad UniversityMarvdashtIran
  7. 7.Department of Clinical Biochemistry, School of MedicineMedical University CampusKermanIran
  8. 8.Immunology Research CenterTabriz University of Medical SciencesTabrizIran
  9. 9.Department of Obstetrics and Gynecology, School of MedicineZahedan University of Medical SciencesZahedanIran
  10. 10.Department of Clinical Biochemistry, School of Medicine, and Cellular and Molecular Research CenterZahedan University of Medical SciencesZahedanIran

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