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Apoptosis

pp 1–3 | Cite as

Correction to: Release of overexpressed CypB activates ERK signaling through CD147 binding for hepatoma cell resistance to oxidative stress

  • Kiyoon Kim
  • Hunsung Kim
  • Kwon Jeong
  • Min Hyung Jung
  • Bum-Soo Hahn
  • Kyung-Sik Yoon
  • Byung Kwan Jin
  • Geon-Ho Jahng
  • Insug Kang
  • Joohun Ha
  • Wonchae Choe
Correction

Correction to: Apoptosis (2012) 17:784–796  https://doi.org/10.1007/s10495-012-0730-5

The original version of this article contained a mistake. The bands for HA Tag and t-ERK in Figs. 2d, 2h, 3d are incorrect. The author informs that these errors had no influence in the scientific content of the paper. The corrected figures (Figs. 2 and 3) are given below.

Fig. 2

Overexpression of CypB protects cells against H2O2-mediated apoptosis. Huh-7 cells were mock-transfected or transfected with a CypB/WT expression construct and treated with 0, 0.6, 0.8 or 1 mM H2O2 for 24 h. After incubation, cell viability was measured by a, left MTT assay and a, right LDH release assay. Apoptotic cells were detected by b PI staining and c annexin V/PI double staining. d Apoptotic markers were analyzed by immunoblotting. Transfected cells were incubated with 0.8 mM H2O2 for 24 h. Whole lysates were separated on 12% SDS-PAGE gels and immunoblotted with anti-PARP, anti-pro-caspase-3, anti-cleaved caspase-3, anti-Bax, anti-Bcl-xL, and anti-HA probe. α-Actinin was used as a loading control. Huh-7 cells were mock-transfected or transfected with a CypB/R95A expression construct and treated with 0, 0.6, 0.8 or 1 mM H2O2 for 24 h. After incubation, cell viability was measured by e MTT assay. Transfected cells were treated with 0.8 mM H2O2. After 24 h of incubation, cells were harvested, and apoptotic cells were detected with f PI staining and g annexin V/PI double staining. h Apoptotic markers were analyzed by immunoblotting. Whole lysates were separated on 10–12% SDS-PAGE gels and immunoblotted with anti-PARP, anti-cleaved caspase 3, anti-Bax, anti-Bcl-xL, and anti-HA probe. α-Actinin was used as a loading control. Data are expressed as mean ± SD of three independent experiments. **P < 0.05 versus mock-transfected cells treated with H2O2; #P < 0.01 versus mock-transfected cells treated with H2O2

Fig. 3

Overexpression of CypB protects Huh-7 cells though ERK activation. a Transfected cells were treated with 0, 0.6, 0.8 or 1 mM H2O2 for 24 h. Protein levels were detected by immunoblotting with anti-phospho ERK, anti-total ERK, abCAM, or anti-HA probe. α-Actinin was used as a loading control. b Transfected Huh-7 cells were pre-treated with the ERK inhibitor PD98059 (50 μM). After a 1-h incubation, cells were treated with 0–1 mL H2O2 for 24 h. Cell viability was measured by MTT assay. Data are expressed as mean ± SD of three independent experiments. *P < 0.05 versus mock-transfected cells treated with H2O2; #P < 0.05 versus mock-transfected cells treated with H2O2 after PD98059 pretreatment. c Apoptotic cells were detected with annexin V/PI double staining. Transfected cells were treated with 0.8 mM H2O2 after a 1-h pretreatment with PD98059. After 24 h, cells were harvested and analyzed by flow cytometry. d ERK upstream activation was determined by Ras affinity assay and immunoblotting. Transfected Huh-7 cell lysates were analyzed by Ras affinity assay and separated on 12% SDS-PAGE gels. Equal protein loading was ensured by Ponceau S staining and pan-Ras immunoblotting of the input. Immunoblotting was performed with antibodies against Raf, phospho-MEK, total-MEK, phospho-ERK, total-ERK, and the HA tag. α-Actinin was used as a loading control. e Huh-7 cells were transfected with con-siRNA or CypB-siRNA and treated with 0.8 mM H2O2 for 24 h. After incubation, apoptotic markers were analyzed by immunoblotting. Whole lysates were separated on 10–12% SDS-PAGE gels and immunoblotted with anti-PARP, anti-pro-caspase-3, anti-Bax, anti-Bcl-xL, anti-phospho ERK and anti-total ERK. f Transfected cells were treated with 0, 0.6, 0.8 or 1 mM H2O2 for 24 h. After incubation, cell viability was measured by LDH-release assay. Data are expressed as mean ± SD of three independent experiments. *P < 0.01 versus con-siRNA transfectants treated with H2O2. g Apoptotic cells were detected with annexin V/PI double staining. After siRNA transfection, cells were incubated with 0 or 0.8 mM H2O2. After 24 h, cells were harvested and analyzed by flow cytometry

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Kiyoon Kim
    • 1
  • Hunsung Kim
    • 1
  • Kwon Jeong
    • 1
  • Min Hyung Jung
    • 2
  • Bum-Soo Hahn
    • 3
    • 4
  • Kyung-Sik Yoon
    • 1
  • Byung Kwan Jin
    • 5
  • Geon-Ho Jahng
    • 6
  • Insug Kang
    • 1
  • Joohun Ha
    • 1
  • Wonchae Choe
    • 1
  1. 1.Department of Biochemistry and Molecular Biology, Medical Science and Engineering Research Center for Bioreaction to Reactive Oxygen Species, Biomedical Science Institute, School of MedicineKyung Hee UniversitySeoulSouth Korea
  2. 2.Department of Obstetrics and Gynecology, School of MedicineKyung Hee UniversitySeoulSouth Korea
  3. 3.National Academy of Agricultural ScienceSuwonSouth Korea
  4. 4.Department of Genetic EngineeringKyung Hee UniversitySuwonSouth Korea
  5. 5.Department of Biochemistry and Molecular Biology, Neurodegeneration Control Research Center, School of MedicineKyung Hee UniversitySeoulSouth Korea
  6. 6.Department of Radiology, Kyung Hee University Hospital-Gangdong, School of MedicineKyung Hee UniversitySeoulSouth Korea

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