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Experimental and Applied Acarology

, Volume 75, Issue 3, pp 333–343 | Cite as

In vitro acaricidal activity of Piper nigrum and Piper longum fruit extracts and their active components against Rhipicephalus (Boophilus) microplus ticks

  • R. Godara
  • M. K. Verma
  • R. Katoch
  • A. Yadav
  • P. Dutt
  • N. K. Satti
  • M. Katoch
Article

Abstract

In vitro acaricidal activity of Piper nigrum and P. longum fruit extracts and their active components (piperine for P. nigrum and piperine and piperlonguminine for P. longum) was evaluated against adults engorged females of Rhipicephalus (Boophilus) microplus using adult immersion test. Three concentrations of each extract with four replications were used in the bioassay. Extracts significantly affected mortality rates of ticks in dose-dependent manner ranged 12.5–95.8% for P. nigrum and 29.2–87.5% for P. longum, with an additional effect on the reproductive physiology of ticks by inhibiting oviposition (28.1–96.9% by P. nigrum and 36.1–89.3% by P. longum). However, the acaricidal and oviposition limiting properties were decreased significantly when the active component(s) of each extract was tested separately. However, the combination of piperine and piperlonguminine (obtained from P. longum extract) caused 79.2% mortality of ticks which is equivalent to the corresponding concentration (~ 5%) of the extract. It can be concluded that the fruit extracts of P. nigrum and P. longum had both acaricidal and oviposition limiting actions against the adults of R. (B.) microplus which could make it a valuable component of developing sustainable strategy for integrated tick management.

Keywords

Piper longum Piper nigrum Piperine Piperlonguminine Rhipicephalus (Boophilus) microplus 

Notes

Acknowledgements

The financial support was provided by Rashtriya Krishi Vikas Yojana (RKVY) Number AUJ/DR/14-15/F-420/7888-7903.

Compliance with ethical standards

Conflict of interest

There is no conflict of interest among the authors.

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Copyright information

© Springer Nature Switzerland AG 2018

Authors and Affiliations

  • R. Godara
    • 1
  • M. K. Verma
    • 2
  • R. Katoch
    • 1
  • A. Yadav
    • 1
  • P. Dutt
    • 2
  • N. K. Satti
    • 2
  • M. Katoch
    • 3
  1. 1.Division of Veterinary Parasitology, Faculty of Veterinary Sciences and Animal HusbandrySher-e-Kashmir University of Agricultural Sciences and TechnologyR.S. Pura, JammuIndia
  2. 2.Analytical Chemistry Division (Instrumentation)NPC, Indian Institute of Integrative Medicine (CSIR)JammuIndia
  3. 3.Microbial Biotechnology DepartmentIndian Institute of Integrative Medicine (CSIR)JammuIndia

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