Mucilaginibacter corticis sp. nov., isolated from bark of Pinus koraiensis

  • Shahina Akter
  • Md. Amdadul HuqEmail author
Original Paper


A gram-stain negative, aerobic, non-motile and rod-shaped novel bacterial strain, designated MAH-19T, was isolated from bark of Pinus koraiensis. The colonies were observed to be light pink coloured, smooth, circular and 0.3–0.7 mm in diameter when grown on R2A agar for 2 days. Strain MAH-19T was found to be able to grow at 10–35 °C (optimum 28–30 °C), at pH 6.0–8.0 (optimum 7.0) and at 0–0.5% NaCl (optimum 0%). Cell growth occurs on nutrient agar and R2A agar. The strain was found to be positive for both catalase and oxidase tests. Cells are able to hydrolyse aesculin and Tween 20, but not casein, gelatin, starch, l-tyrosine, DNA, l-arginine, urea or Tween 80. According to the 16S rRNA gene sequence comparisons, the isolate was identified as a member of the genus Mucilaginibacter and to be closely related to Mucilaginibacter panaciglaebae BXN5-31T (97.4% similarity), Mucilaginibacter antarcticus S14-88T (97.2%) and Mucilaginibacter ximonensis XM-003T (97.1%). In DNA–DNA hybridization tests, the DNA relatedness between strain MAH-19T and its close phylogenetic neighbours was below 45.0%. The novel strain MAH-19T has a draft genome size of 5,335,442 bp (14 contigs), annotated with 4963 protein-coding genes, 44 tRNA and 6 rRNA genes. The genomic DNA G+C content was determined to be 42.7 mol%. The predominant isoprenoid quinone of strain MAH-19T was identified as MK-7. The major fatty acids were identified as C15:0 iso and summed feature 3 (comprising C16:1ω7c and/or C16:1ω6c). The DNA–DNA hybridization results and results of the genotypic analysis, in combination with chemotaxonomic and physiological data, demonstrated that strain MAH-19T represents a novel species within the genus Mucilaginibacter, for which the name Mucilaginibacter corticis sp. nov. is proposed, with MAH-19T (= KACC 19745T = CGMCC1.13657T) as the type strain.


Mucilaginibacter corticis Gram-staining negative 16S rRNA gene Fatty acid 



This study was performed with the support of the National Research Foundation (NRF) of Korea Grant (Project No. NRF-2018R1C1B5041386, Recipient: Md. Amdadul Huq) funded by Korean Government, Republic of Korea.

Author’s contributions

Md. Amdadul Huq conceived the original screening and research plans. Md. Amdadul Huq supervised the experiments and wrote the article with contributions of all the authors. Md. Amdadul Huq and Shahina Akter performed all of the experiments.

Compliance with ethical standards

Conflict of interest

All authors declare that they have no conflict of interest.

Supplementary material

10482_2019_1358_MOESM1_ESM.pptx (46 kb)
Supplementary Table 1Negative properties of strain MAH-19T carried out by commercial test kits (API 20NE and API ZYM). (PPTX 46 kb)
10482_2019_1358_MOESM2_ESM.pptx (368 kb)
Supplementary material 2 (PPTX 368 kb)
10482_2019_1358_MOESM3_ESM.pptx (87 kb)
Supplementary material 3 (PPTX 87 kb)


  1. An DS, Yin CR, Lee ST, Cho CH (2009) Mucilaginibacter daejeonensis sp. nov., isolated from dried rice straw. Int J Syst Evol Microbiol 59:1122–1125CrossRefGoogle Scholar
  2. Baik KS, Park SC, Kim EM, Lim CH, Seong CN (2010) Mucilaginibacter rigui sp. nov., isolated from wetland freshwater and emended description of the genus Mucilaginibacter. Int J Syst Evol Microbiol 60:134–139CrossRefGoogle Scholar
  3. Chen X, Zhao R, Tian Y, Kong B, Li X, Chen Z, Li Y (2014) Mucilaginibacter polytrichastri sp. nov., isolated from a moss (Polytrichastrum formosum) and emended description of the genus Mucilaginibacter. Int J Syst Evol Microbiol 64:1395–1400CrossRefGoogle Scholar
  4. Collins MD, Jones D (1981) Distribution of isoprenoid quinone structural types in bacteria and their taxonomic implications. Microbiol Rev 45:316–354PubMedPubMedCentralGoogle Scholar
  5. Ezaki T, Hashimoto Y, Yabuuchi E (1989) Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229CrossRefGoogle Scholar
  6. Fautz E, Reichenbach H (1980) A simple test for flexirubin-type pigments. FEMS Microbiol Lett 8:87–91CrossRefGoogle Scholar
  7. Felsenstein J (1985) Confidence limit on phylogenies: an approach using the bootstrap. Evol Evol Int J Org Evol 39:783–791CrossRefGoogle Scholar
  8. Gillis M, De Ley J, De Cleene M (1970) The determination of molecular weight of bacterial genome DNA from renaturation rates. Eur J Biochem 12:143–153CrossRefGoogle Scholar
  9. Hall TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucl Acids Symp Ser 41:95–98Google Scholar
  10. Hiraishi A, Ueda Y, Ishihara J, Mori T (1996) Comparative lipoquinone analysis of influent sewage and activated sludge by high-performance liquid chromatography and photodiode array detection. J Gen Appl Microbiol 42:457–469CrossRefGoogle Scholar
  11. Huq MA (2017) Chryseobacterium chungangensis sp. nov., a bacterium isolated from soil of sweet gourd garden. Arch Microbiol. CrossRefPubMedGoogle Scholar
  12. Huq MA (2018) Caenispirillum humi sp. nov., a bacterium isolated from the soil of Korean pine garden. Arch Microbiol 200:343–348CrossRefGoogle Scholar
  13. Jeon Y, Lee SS, Chung BS, Kim JM, Bae JW, Park SK, Jeon CO (2009) Mucilaginibacter oryzae sp. nov., isolated from soil of a rice paddy. Int J Syst Evol Microbiol 59:1451–1454CrossRefGoogle Scholar
  14. Joung Y, Joh K (2011) Mucilaginibacter myungsuensis sp. nov., isolated from a mesotrophic artificial lake. Int J Syst Evol Microbiol 61:1506–1510CrossRefGoogle Scholar
  15. Joung Y, Kim H, Kang H, Lee BI, Ahn TS, Joh K (2014) Mucilaginibacter soyangensis sp. nov., isolated from a lake. Int J Syst Evol Microbiol 64:413–419CrossRefGoogle Scholar
  16. Kang SJ, Jung YT, Oh KH, Oh TK, Yoon JH (2011) Mucilaginibacter boryungensis sp. nov., isolated from soil. Int J Syst Evol Microbiol 61:1549–1553CrossRefGoogle Scholar
  17. Khan H, Chung EJ, Jeon CO, Chung YR (2013) Mucilaginibacter gynuensis sp. nov., isolated from rotten wood. Int J Syst Evol Microbiol 63:3225–3231CrossRefGoogle Scholar
  18. Kim BC, Lee KH, Kim MN, Lee J, Shin KS (2010) Mucilaginibacter dorajii sp. nov., isolated from the rhizosphere of Platycodon grandiflorum. FEMS Microbiol Lett 309:130–135PubMedGoogle Scholar
  19. Kim OS, Cho YJ, Lee K, Yoon SH, Kim M, Na H, Park SC, Jeon YS, Lee JH, Yi H, Won S, Chun J (2012) Introducing EzTaxon-e: a prokaryotic 16S rRNA Gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62:716–721CrossRefGoogle Scholar
  20. Kimura M (1983) The neutral theory of molecular evolution. Cambridge University Press, CambridgeCrossRefGoogle Scholar
  21. Lane DJ (1991) 16S/23S rRNA sequencing. In: Stackebrandt E, Goodfellow M (eds) Nucleic acid techniques in bacterial systematic. Wiley, New York, pp 115–175Google Scholar
  22. Lee JH, Kim MS, Kang JW, Baik KS, Seong CN (2016) Mucilaginibacter puniceus sp. nov., isolated from wetland freshwater. Int J Syst Evol Microbiol 66:4549–4554CrossRefGoogle Scholar
  23. Lee SY, Siddiqi MZ, Kim SY, Yu HS, Lee JH, Im WT (2018) Mucilaginibacter panaciglaebae sp. nov., isolated from soil of a ginseng field. Int J Syst Evol Microbiol 68:149–154CrossRefGoogle Scholar
  24. Luo X, Zhang L, Dai J, Liu M, Zhang K, An H, Fang C (2009) Mucilaginibacter ximonensis sp. nov., isolated from Tibetan soil. Int J Syst Evol Microbiol 59:1447–1450CrossRefGoogle Scholar
  25. Madhaiyan M, Poonguzhali S, Lee JS, Senthilkumar M, Lee KC, Sundaram S (2010) Mucilaginibacter gossypii sp. nov. and Mucilaginibacter gossypiicola sp. nov., plant-growth-promoting bacteria isolated from cotton rhizosphere soils. Int J Syst Evol Microbiol 60:2451–2457CrossRefGoogle Scholar
  26. Männistö MK, Tiirola M, McConnell J, Haggblom MM (2010) Mucilaginibacter frigoritolerans sp. nov., Mucilaginibacter lappiensis sp. nov. and Mucilaginibacter mallensis sp. nov., isolated from soil and lichen samples. Int J Syst Evol Microbiol 60:2849–2856CrossRefGoogle Scholar
  27. McConaughy BL, Laird CD, McCarthy BJ (1969) Nucleic acid reassociation in formamide. Biochemistry 8:3289–3295CrossRefGoogle Scholar
  28. Pankratov TA, Tindall BJ, Liesack W, Dedysh SN (2007) Mucilaginibacter paludis gen. nov., sp. nov. and Mucilaginibacter gracilis sp. nov., pectin-, xylan- and laminarin-degrading members of the family Sphingobacteriaceae from acidic Sphagnum peat bog. Int J Syst Evol Microbiol 57:2349–2354CrossRefGoogle Scholar
  29. Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425Google Scholar
  30. Sasser M (1990) Identification of bacteria by gas chromatography of cellular fatty acids. MIDI Technical Note 101. Newark, DE: MIDI IncGoogle Scholar
  31. Stabili L, Gravili C, Tredici SM, Piraino S, Talà A, Boero F, Alifano P (2008) Epibiotic Vibrio luminous bacteria isolated from some hydrozoa and bryozoa species. Microbiol Ecol 56:625–636CrossRefGoogle Scholar
  32. Stackebrandt E, Goebel BM (1994) Taxonomic note: a place for DNA–DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849CrossRefGoogle Scholar
  33. Tamaoka J, Katayama-Fujiruma A, Kuraishi H (1983) Analysis of bacterial menaquinone mixtures by high performance liquid chromatography. J Appl Bacteriol 54:31–36CrossRefGoogle Scholar
  34. Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739CrossRefGoogle Scholar
  35. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG (1997) The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882CrossRefGoogle Scholar
  36. Urai M, Aizawa T, Nakagawa Y, Nakajima M, Sunairi M (2008) Mucilaginibacter kameinonensis sp., nov., isolated from garden soil. Int J Syst Evol Microbiol 58:2046–2050CrossRefGoogle Scholar
  37. Wayne LG, Brenner DJ, Colwell RR, Grimont PAD, Kandler O, Krichevsky MI, Moore LH, Moore WEC, Murray RGE et al (1987) International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464CrossRefGoogle Scholar
  38. Yan YQ, Hao YX, He RH, Du ZJ (2019) Mucilaginibacter gilvus sp. nov., isolated from Antarctic soil. Int J Syst Evol Microbiol. CrossRefPubMedGoogle Scholar
  39. Yoon JH, Kang SJ, Park S, Oh TK (2012) Mucilaginibacter litoreus sp. nov., isolated from marine sand. Int J Syst Evol Microbiol 62:2822–2827CrossRefGoogle Scholar
  40. Zheng R, Zhao Y, Wang L, Chang X, Zhang Y, Da X, Peng F (2016) Mucilaginibacter antarcticus sp. nov., isolated from tundra soil. Int J Syst Evol Microbiol 66:5140–5144CrossRefGoogle Scholar
  41. Zhou Z, Dong Y, Xia X, Wu S, Huang Y, Liao S, Wang G (2019) Mucilaginibacter terrenus sp. nov., isolated from manganese mine soil. Int J Syst Evol Microbiol 69:3074–3079CrossRefGoogle Scholar

Copyright information

© Springer Nature Switzerland AG 2019

Authors and Affiliations

  1. 1.Department of Food Science and Biotechnology, College of BioNano TechnologyGachon UniversitySeongnamRepublic of Korea
  2. 2.Department of Food and Nutrition, College of Biotechnology and Natural ResourceChung-Ang UniversityAnseong-siRepublic of Korea

Personalised recommendations