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Mucilaginibacter corticis sp. nov., isolated from bark of Pinus koraiensis

  • Shahina Akter
  • Md. Amdadul HuqEmail author
Original Paper
  • 14 Downloads

Abstract

A gram-stain negative, aerobic, non-motile and rod-shaped novel bacterial strain, designated MAH-19T, was isolated from bark of Pinus koraiensis. The colonies were observed to be light pink coloured, smooth, circular and 0.3–0.7 mm in diameter when grown on R2A agar for 2 days. Strain MAH-19T was found to be able to grow at 10–35 °C (optimum 28–30 °C), at pH 6.0–8.0 (optimum 7.0) and at 0–0.5% NaCl (optimum 0%). Cell growth occurs on nutrient agar and R2A agar. The strain was found to be positive for both catalase and oxidase tests. Cells are able to hydrolyse aesculin and Tween 20, but not casein, gelatin, starch, l-tyrosine, DNA, l-arginine, urea or Tween 80. According to the 16S rRNA gene sequence comparisons, the isolate was identified as a member of the genus Mucilaginibacter and to be closely related to Mucilaginibacter panaciglaebae BXN5-31T (97.4% similarity), Mucilaginibacter antarcticus S14-88T (97.2%) and Mucilaginibacter ximonensis XM-003T (97.1%). In DNA–DNA hybridization tests, the DNA relatedness between strain MAH-19T and its close phylogenetic neighbours was below 45.0%. The novel strain MAH-19T has a draft genome size of 5,335,442 bp (14 contigs), annotated with 4963 protein-coding genes, 44 tRNA and 6 rRNA genes. The genomic DNA G+C content was determined to be 42.7 mol%. The predominant isoprenoid quinone of strain MAH-19T was identified as MK-7. The major fatty acids were identified as C15:0 iso and summed feature 3 (comprising C16:1ω7c and/or C16:1ω6c). The DNA–DNA hybridization results and results of the genotypic analysis, in combination with chemotaxonomic and physiological data, demonstrated that strain MAH-19T represents a novel species within the genus Mucilaginibacter, for which the name Mucilaginibacter corticis sp. nov. is proposed, with MAH-19T (= KACC 19745T = CGMCC1.13657T) as the type strain.

Keywords

Mucilaginibacter corticis Gram-staining negative 16S rRNA gene Fatty acid 

Notes

Acknowledgement

This study was performed with the support of the National Research Foundation (NRF) of Korea Grant (Project No. NRF-2018R1C1B5041386, Recipient: Md. Amdadul Huq) funded by Korean Government, Republic of Korea.

Author’s contributions

Md. Amdadul Huq conceived the original screening and research plans. Md. Amdadul Huq supervised the experiments and wrote the article with contributions of all the authors. Md. Amdadul Huq and Shahina Akter performed all of the experiments.

Compliance with ethical standards

Conflict of interest

All authors declare that they have no conflict of interest.

Supplementary material

10482_2019_1358_MOESM1_ESM.pptx (46 kb)
Supplementary Table 1Negative properties of strain MAH-19T carried out by commercial test kits (API 20NE and API ZYM). (PPTX 46 kb)
10482_2019_1358_MOESM2_ESM.pptx (368 kb)
Supplementary material 2 (PPTX 368 kb)
10482_2019_1358_MOESM3_ESM.pptx (87 kb)
Supplementary material 3 (PPTX 87 kb)

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Copyright information

© Springer Nature Switzerland AG 2019

Authors and Affiliations

  1. 1.Department of Food Science and Biotechnology, College of BioNano TechnologyGachon UniversitySeongnamRepublic of Korea
  2. 2.Department of Food and Nutrition, College of Biotechnology and Natural ResourceChung-Ang UniversityAnseong-siRepublic of Korea

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