Streptomyces tibetensis sp. nov., an actinomycete isolated from the Tibetan Plateau
A novel actinomycete, designated strain XZ 46T, was isolated from acid sandy soil collected from the Tibetan Plateau, China. Its taxonomic position was determined using a polyphasic approach. Strain XZ 46T shows the typical morphological and chemotaxonomic features of members of the genus Streptomyces: slightly yellow to brown substrate mycelia and grayish white to slightly yellow aerial hyphae forming cylindrical and spiny spores; meso-diaminopimelic acid in the cell wall peptidoglycan; MK-9(H8), MK-9(H4) and MK-9(H2) as predominant menaquinones; diphosphatidylglycerol, phospatidylethanolamine, phosphatidylglycerol and phosphatidylinositol as main polar lipids; and iso-C15:0, iso-C16:0 and anteiso-C15:0 as major cellular fatty acids. The G+C content of the draft genome sequence, consisting of 8,995,813 bp, is 71.23%. The16S rRNA gene sequence analysis indicated that strain XZ 46T shows high sequence similarity to Streptomyces luteogriseus NBRC 13402T as well as forming an independent lineage clade with it in phylogenetic trees. Multilocus sequence analysis (MLSA) of five housekeeping genes (atpD, gyrB, recA, rpoB and trpB) illustrated that Streptomyces hawaiiensis is also a very closely related taxon. However, DNA–DNA hybridization, MLSA evolutionary distance and phenotypic properties demonstrate that strain XZ 46T can be distinguished from these phylogenetically related Streptomyces species. Therefore, it is concluded that strain XZ 46T represents a novel species of the genus Streptomyces, for which the name Streptomyces tibetensis is sp. nov. proposed. The type strain is XZ 46T (= CGMCC 4.7579T = KCTC 49221T).
KeywordsActinomycete Polyphasic Streptomyces tibetensis Soil
Thanks to National Natural Science Foundation of China (Grant No. U1806222), Shandong Provincial Natural Science Joint Found With Universities and Scientific Research Institution (ZR2018LC001).
JL and LW conducted the experiments. ZY and LL contributed to writing. YL was in charge of experimental expenditure.
Compliance with ethical standards
Conflicts of interest
The authors declare that there are no conflicts of interest.
- Collee JG, Miles RS, Watt B (1996) Tests for identification of bacteria. In: Collee JG, Fraser AG, Marmion BP, Simmons A (eds) Practical medical microbiology, 14th edn. Churchill Livingstone, New York, pp 131–149Google Scholar
- Hsu SC, Lockwood JL (1975) Powdered chitin agar as selective medium for enumeration of actinomycetes in water and soil. Appl Microbial 29:422–426Google Scholar
- Kelly KL (1964) Inter-society color council-national bureau of standards color-name charts illustrated with centroid colors. US Government Printing Office, Washington, DCGoogle Scholar
- Labeda DP, Goodfellow M, Brown R, Ward AC, Lanoot B, Vanncanneyt M, Swings J, Kim SB, Liu Z, Chun J, Tamura T, Oguchi A, Kikuchi T, Kikuchi H, Nishii T, Tsuji K, Yamaguchi Y, Tase A, Takahashi M, Sakane T, Suzuki KI, Hatano K (2012) Phylogenetic study of the species within the family Streptomycetaceae. Antonie Van Leeuwenhoek 101:73–104CrossRefGoogle Scholar
- Labeda DP, Doroghazi JP, Ju K-S, Metcalf WW (2014) Taxonomic evaluation of Streptomyces albus and related species using multi-locus sequence analysis and proposals to emend the description of Streptomyces albus and describe Streptomyces pathocidini sp. nov. Int J Syst Evol Microbiol 64:894–900CrossRefGoogle Scholar
- Lechevalier MP, Lechevalier HA (1970) Chemical composition as a criterion in the classification of aerobic actinomycetes. Int J Syst Evol Microbiol 20:435–443Google Scholar
- Saitou N, Nei M (1987) The neighbor joining method: a new method or reconstructing phylogenetic trees. Mol Biol Evol 4:406–425Google Scholar
- Sasser M (1990) Identification of bacteria by gas chromatography of cellular fatty acids, MIDI technical note 101. MIDI Inc, NewarkGoogle Scholar
- Tadashi A (1975) Culture media for actinomycetes. The Society for Actinomycetes, TokyoGoogle Scholar
- Tindall BJ, Sikorski J, Smibert RM, Krieg NR (2007) Phenotypic characterization and the principles of comparative systematics. In: Reddy CA, Beveridge TJ, Breznak JA, Marzluf G, Schmidt TM, et al. (eds) Methods for general and molecular microbiology, 3rd edn. ASM Press, Washington, DC, pp 330–393Google Scholar
- Wayne LG, Brenner DJ, Colwell RR, Grimont PAD, Kandler O, Krichevsky MI, Moore LH, Moore WEC, Murray RGE (1987) International committee on systematic bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:464Google Scholar
- Williams ST, Goodfellow M, Alderson G, Wellington EM, Sneath PH, Sackin MJ (1983) Numerical classification of Streptomyces and related genera. J Gen Microbiol 129:1743–1813Google Scholar