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Corallincola luteus sp. nov., a marine bacterium isolated from surface sediment of Bohai Sea of China

  • Qiaomeng Ren
  • Xiaoting Yang
  • Min YuEmail author
  • Xiaorong Tian
  • Bei Li
  • Zhe Meng
  • Yanhong Wu
  • Jiwen Liu
  • Xiao-Hua Zhang
Original Paper
  • 12 Downloads

Abstract

A novel Gram-stain negative, strictly aerobic, rod-shaped motile bacterium with a single flagellum, designated strain DASS28T, was isolated from surface sediment of Bohai Sea in China. Growth occurred in the presence of 1.0–4.0% NaCl (w/v, optimum 2.0%), at 10–37 °C (optimum 20 °C) on the Marine agar 2216E and pH 6.0–10.0 (optimum pH 8.0). The major fatty acids (> 10% of total fatty acids) were summed feature 3 (C16:1ω7c and/or iso-C15:0 2-OH), C16:0 and C18:1ω7c. The polar lipids were phosphatidylglycerol, phosphatidylethanolamine, an unidentified phospholipid, an unidentified aminolipid and two unidentified polar lipids. The major respiratory quinone was ubiquinone-8 (Q-8). The genomic DNA G + C content calculated from the genome sequence of strain DASS28T was 48.8 mol%. Phylogenetic analysis based on 16S rRNA gene sequence indicated that strain DASS28T belongs to the genus Corallincola and shows high 16S rRNA gene sequence similarity of 96.7% to Corallincola platygyrae JLT 2006T (= JCM18796T = CGMCC 1.10992T). On the basis of the polyphasic evidence, strain DASS28T is considered to represent a novel species in the genus Corallincola, for which the name Corallincola luteus sp. nov. is proposed. The type strain is DASS28T (= KCTC 52376T = MCCC 1K03208T).

Keywords

Corallincola luteus Surface sediment Bohai Sea 

Abbreviations

HPLC

High performance liquid chromatography

JCM

Japan Collection of Microorganisms

MK-7

Menaquinone 7

Q-8

Ubiquinone-8

PG

Phosphatidylglycerol

PE

Phosphatidylethanolamine

PL

Phospholipid

AL

Aminolipid

UL

Unidentified polar lipid

Notes

Acknowledgements

We are grateful to the captain and crew of the R/V Dong Fang Hong 2 for their assistance during the cruise and the kind help of Prof. Aharon Oren for the naming of the novel strain DASS28T. This work was supported by projects from the National Key Research and Development Program of China (No. 2016YFA0601303) and the National Natural Science Foundation of China (Nos. 41476112 and 41521064).

Authors’ contribution

Qiaomeng Ren and Xiaoting Yang carried out the laboratory work, participated in the data analyzation and drafted the manuscript. Min Yu, Xiaorong Tian and Bei Li participated in the design of the study and helped to draft the manuscript. Zhe Meng, Yanhong Wu and Jiwen Liu participated in the data analyzation. Xiao-Hua Zhang conceived the study, and participated in its design and coordination and helped to draft the manuscript. All authors read and approved the final manuscript.

Conflicts of interest

There is no conflict of interest about this passage.

Supplementary material

10482_2019_1295_MOESM1_ESM.pdf (1 mb)
Supplementary material 1 (PDF 1058 kb)

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Copyright information

© Springer Nature Switzerland AG 2019

Authors and Affiliations

  • Qiaomeng Ren
    • 1
  • Xiaoting Yang
    • 1
  • Min Yu
    • 1
    • 2
    • 3
    Email author
  • Xiaorong Tian
    • 1
  • Bei Li
    • 1
  • Zhe Meng
    • 1
  • Yanhong Wu
    • 1
  • Jiwen Liu
    • 1
  • Xiao-Hua Zhang
    • 1
    • 2
    • 3
  1. 1.College of Marine Life SciencesOcean University of ChinaQingdaoChina
  2. 2.Laboratory for Marine Ecology and Environmental ScienceQingdao National Laboratory for Marine Science and TechnologyQingdaoChina
  3. 3.Institute of Evolution and Marine BiodiversityOcean University of ChinaQingdaoChina

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