Corallincola luteus sp. nov., a marine bacterium isolated from surface sediment of Bohai Sea of China

  • Qiaomeng Ren
  • Xiaoting Yang
  • Min YuEmail author
  • Xiaorong Tian
  • Bei Li
  • Zhe Meng
  • Yanhong Wu
  • Jiwen Liu
  • Xiao-Hua Zhang
Original Paper


A novel Gram-stain negative, strictly aerobic, rod-shaped motile bacterium with a single flagellum, designated strain DASS28T, was isolated from surface sediment of Bohai Sea in China. Growth occurred in the presence of 1.0–4.0% NaCl (w/v, optimum 2.0%), at 10–37 °C (optimum 20 °C) on the Marine agar 2216E and pH 6.0–10.0 (optimum pH 8.0). The major fatty acids (> 10% of total fatty acids) were summed feature 3 (C16:1ω7c and/or iso-C15:0 2-OH), C16:0 and C18:1ω7c. The polar lipids were phosphatidylglycerol, phosphatidylethanolamine, an unidentified phospholipid, an unidentified aminolipid and two unidentified polar lipids. The major respiratory quinone was ubiquinone-8 (Q-8). The genomic DNA G + C content calculated from the genome sequence of strain DASS28T was 48.8 mol%. Phylogenetic analysis based on 16S rRNA gene sequence indicated that strain DASS28T belongs to the genus Corallincola and shows high 16S rRNA gene sequence similarity of 96.7% to Corallincola platygyrae JLT 2006T (= JCM18796T = CGMCC 1.10992T). On the basis of the polyphasic evidence, strain DASS28T is considered to represent a novel species in the genus Corallincola, for which the name Corallincola luteus sp. nov. is proposed. The type strain is DASS28T (= KCTC 52376T = MCCC 1K03208T).


Corallincola luteus Surface sediment Bohai Sea 



High performance liquid chromatography


Japan Collection of Microorganisms


Menaquinone 7












Unidentified polar lipid



We are grateful to the captain and crew of the R/V Dong Fang Hong 2 for their assistance during the cruise and the kind help of Prof. Aharon Oren for the naming of the novel strain DASS28T. This work was supported by projects from the National Key Research and Development Program of China (No. 2016YFA0601303) and the National Natural Science Foundation of China (Nos. 41476112 and 41521064).

Authors’ contribution

Qiaomeng Ren and Xiaoting Yang carried out the laboratory work, participated in the data analyzation and drafted the manuscript. Min Yu, Xiaorong Tian and Bei Li participated in the design of the study and helped to draft the manuscript. Zhe Meng, Yanhong Wu and Jiwen Liu participated in the data analyzation. Xiao-Hua Zhang conceived the study, and participated in its design and coordination and helped to draft the manuscript. All authors read and approved the final manuscript.

Conflicts of interest

There is no conflict of interest about this passage.

Supplementary material

10482_2019_1295_MOESM1_ESM.pdf (1 mb)
Supplementary material 1 (PDF 1058 kb)


  1. Auman AJ, Breezee JL, Gosink JJ, Kampfer P, Staley JT et al (2006) Psychromonas ingrahamii sp. nov., a novel gas vacuolate, psychrophilic bacterium isolated from Arctic polar sea ice. Int J Syst Evol Microbiol 56:1001–1007CrossRefGoogle Scholar
  2. Auman AJ, Breezee JL, Gosink JJ, Schumann P, Barnes CR et al (2010) Psychromonas boydii sp. nov., a gas-vacuolate, psychrophilic bacterium isolated from an Arctic sea-ice core. Int J Syst Evol Microbiol 60:84–92CrossRefGoogle Scholar
  3. Ausubel F, Brent R, Kingston R, Moore D, Seidman J et al (1995) Short protocols in molecular biology: a compendium of methods from current protocols in molecular biology, 3rd edn. Wiley, New YorkGoogle Scholar
  4. Aziz RK, Bartels D, Best AA, DeJongh M, Disz T et al (2008) The RAST Server: rapid annotations using subsystems technology. BMC Genom 9:75CrossRefGoogle Scholar
  5. Bernardet JF, Nakagawa Y, Holmes B (2002) Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 52(3):1049–1070Google Scholar
  6. Beveridge TJ, Lawrence JR, Murray RG (2007) Sampling and staining for light microscopy. In: Reddy CA, Beveridge TJ, Breznak TA, Marzluf G, Schmidt TM, Snyder LR (eds) Methods for general and molecular microbiology. American Society for Microbiology, Washington, DC, pp 19–33Google Scholar
  7. Bowman JP, McMeekin TA (2005) Alteromonadales ord. nov.. In: Garrity GM (ed) Bergey’s manual of systematics of bacteriology, vol 2. Springer, New York, pp 443–491CrossRefGoogle Scholar
  8. Collins MD, Shah HN (1984) Fatty acid, menaquinone and polar lipid composition of Rothia dentocariosa. Arch Microbiol 137:247–249CrossRefGoogle Scholar
  9. Groudieva T, Grote R, Antranikian G (2003) Psychromonas arctica sp. nov., a novel psychrotolerant, biofilm-forming bacterium isolated from Spitzbergen. Int J Syst Evol Microbiol 53(2):539–545CrossRefGoogle Scholar
  10. Hsu S, Lockwood J (1975) Powdered chitin agar as a selective medium for enumeration of Actinomycetes in water and soil. Appl Microbiol 29:422–426Google Scholar
  11. Ivanova EP, Gorshkova NM, Bowman JP, Lysenko AM, Zhukova NV et al (2004) Shewanella pacifica sp. nov., a polyunsaturated fatty acid-producing bacterium isolated from sea water. Syst Evol Microbiol 54(4):1083–1087CrossRefGoogle Scholar
  12. Kimura M (1980) A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120CrossRefGoogle Scholar
  13. Komagata K, Suzuki KI (1988) 4 Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19:161–207CrossRefGoogle Scholar
  14. Kumar S, Stecher G, Tamura K (2016) MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 33(7):1870–1874CrossRefGoogle Scholar
  15. Larkin MA, Blackshields G, Brown NP, Chenna R, McGettigan PA et al (2007) CLUSTAL W and CLUSTAL X version 2.0. Bioinformatics 23:2947–2948CrossRefGoogle Scholar
  16. Li Y, Chan Y, Fu Y (2013) Coralslurrinella hongkonensis gen. nov., sp. nov., anovel bacterium in the family Psychromonadaceae, isolated from the coral Platygyra carnosus. Antonie Van Leeuwenhoek 104(6):983–991CrossRefGoogle Scholar
  17. Minnikin D, O’donnell A, Goodfellow M, Alderson G, Athalye M et al (1984) Anintegrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241CrossRefGoogle Scholar
  18. Moore ERB, Arnscheidt A, Krüger A, Strömpl C, Mau M et al (1999) Simplified protocols for the preparation of genomic DNA from bacterial cultures. Mol Microb Ecol Man 1(1):1–15Google Scholar
  19. Mountfort DO, Rainey FA, Burghardt J, Kaspar HF, Stackebrandt E et al (1998) Psychromonas antarcticus gen. nov., sp. nov., a new aerotolerant anaerobic, halophilic psychrophile isolated from pond sediment of the McMurdo Ice Shelf, Antarctica. Arch Microbiol 169(3):231–238CrossRefGoogle Scholar
  20. Sasser M (1990) Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. MIDI Inc, NewarkGoogle Scholar
  21. Stackebrandt E, Goebel BM (1994) Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Evol Microbiol 44:846–849CrossRefGoogle Scholar
  22. Tindall BJ, Sikorski J, Smibert RA, Krieg NR (2007) Phenotypic characterization and the principles of comparative systematics. In: Reddy CA, Beveridge TJ, Breznak JA, Marzluf G, Schmidt TM, Snyder LR (eds) Methods for general and molecular microbiology. American Society for Microbiology, Washington, DC, pp 330–393Google Scholar
  23. Tritt A, Eisen JA, Facciotti MT, Darling AE (2012) An integrated pipeline for denovo assembly of microbial genomes. PLoS ONE 7(9):e42304CrossRefGoogle Scholar
  24. Xie CH, Yokota A (2003) Phylogenetic analyses of Lampropedia hyalina based on the 16S rRNA gene sequence. J Gen Appl Microbiol 49(6):345–349CrossRefGoogle Scholar
  25. Yoon JH, Lee KC, Kho YH, Kang KH, Kim CJ et al (2002) Halomonas alimentaria sp. nov., isolated from jeotgal, a traditional Korean fermented seafood. Int J Syst Evol Microbiol 52:123–130CrossRefGoogle Scholar
  26. Yoon SH, Ha SM, Kwon S, Lim J, Kim Y et al (2017) Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol 67:1613–1617CrossRefGoogle Scholar
  27. Zhang Z, Yu T, Xu T, Zhang XH (2014) Aquimarina pacifica sp. nov., isolated from seawater. Int J Syst Evol Microbiol 64:1991–1997CrossRefGoogle Scholar

Copyright information

© Springer Nature Switzerland AG 2019

Authors and Affiliations

  • Qiaomeng Ren
    • 1
  • Xiaoting Yang
    • 1
  • Min Yu
    • 1
    • 2
    • 3
    Email author
  • Xiaorong Tian
    • 1
  • Bei Li
    • 1
  • Zhe Meng
    • 1
  • Yanhong Wu
    • 1
  • Jiwen Liu
    • 1
  • Xiao-Hua Zhang
    • 1
    • 2
    • 3
  1. 1.College of Marine Life SciencesOcean University of ChinaQingdaoChina
  2. 2.Laboratory for Marine Ecology and Environmental ScienceQingdao National Laboratory for Marine Science and TechnologyQingdaoChina
  3. 3.Institute of Evolution and Marine BiodiversityOcean University of ChinaQingdaoChina

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