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Vibrio profundi sp. nov., isolated from a deep-sea seamount

  • Nai-Xing Zhang
  • De-Chao ZhangEmail author
  • Neng-Hu Qiao
Original Paper
  • 22 Downloads

Abstract

A Gram-stain negative, rod-shaped, facultative anaerobic, motile bacterial strain, designated TP187T, was isolated from a seamount near the Yap Trench in the tropical western Pacific. Phylogenetic analysis based on the 16S rRNA gene sequence showed that strain TP187T is related to members of the genus Vibrio and has high 16S rRNA gene sequence similarity with the type strains of Vibrio chagasii (97.3%) and Vibrio gallaecicus (97.1%). Sequence similarities to all other type strains of current species of the genus Vibrio were below 97%. The polar lipids profile was found to contain diphosphatidylglycerol, phosphatidylglycerol, an aminophospholipid, two aminolipids, four phospholipids and eleven unidentified polar lipids. Ubiquinone Q-8 was detected as the predominant quinone. The genomic DNA G + C content of strain TP187T was determined to be 43.7 mol%. In addition, the maximum values of in silico DNA–DNA hybridization (isDDH) and average nucleotide identity (ANI) between strain TP187T with V. chagasii LMG 21353T were 22.40 and 77.50% respectively. Both values are below the proposed cutoff levels for species delineation, i.e. 70 and 95%, respectively. Combined data from phenotypic, phylogenetic, isDDH and ANI data demonstrated that the strain TP187T is representative of a novel species of the genus Vibrio, for which we propose the name Vibrio profundi sp. nov. (type strain TP187T = KACC 18555T = CGMCC 1.15395T).

Keywords

Vibrio Coral Seamount 

Notes

Acknowledgements

This work was supported by the Senior User Project of RV KEXUE (KEXUE2019G09), the Science & Technology Basic Resources Investigation Program of China (2017FY100804) and the Open Fund of Shandong Provincial Key Laboratory of Marine Ecological Environment and Disaster Prevention and Mitigation under contract No. 201802. We are grateful for Center for Ocean Mega-Science, Chinese Academy of Sciences.

Authors’ contributions

D-C Zhang designed the project and took samples. N-X Zhang performed lab work, N-X Zhang and N–H Qiao analysed data and wrote the manuscript. D-C Zhang revised the manuscript.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants performed by any of the authors.

Supplementary material

10482_2019_1286_MOESM1_ESM.docx (801 kb)
Supplementary material 1 (DOCX 801 kb)

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Copyright information

© Springer Nature Switzerland AG 2019

Authors and Affiliations

  • Nai-Xing Zhang
    • 1
    • 2
  • De-Chao Zhang
    • 3
    • 4
    • 5
    Email author
  • Neng-Hu Qiao
    • 6
    • 4
  1. 1.Shandong Provincial Key Laboratory of Marine Ecological Environment and Disaster Prevention and MitigationQingdaoPeople’s Republic of China
  2. 2.North China Sea Forecast CenterSOAQingdaoPeople’s Republic of China
  3. 3.Institute of OceanologyChinese Academy of SciencesQingdaoPeople’s Republic of China
  4. 4.University of Chinese Academy of SciencesBeijingPeople’s Republic of China
  5. 5.Center for Ocean Mega-ScienceChinese Academy of SciencesQingdaoPeople’s Republic of China
  6. 6.State Key Laboratory of Urban and Regional Ecology, Research Center for Eco-Environmental SciencesChinese Academy of SciencesBeijingPeople’s Republic of China

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