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Microvirga calopogonii sp. nov., a novel alphaproteobacterium isolated from a root nodule of Calopogonium mucunoides in Southwest China

  • Fang Wang
  • Lei Yang
  • Jia Deng
  • Xiaoyun Liu
  • Yanyuan Lu
  • Wenxin Chen
  • Jianrong WuEmail author
Original Paper
  • 18 Downloads

Abstract

In this study, a Gram-negative, rod-shaped, and non-spore-forming bacterium, which was designated as strain CCBUA 65841T, was isolated from a root nodule of Calopogonium mucunoides grown in Yunan Province of China. The sequence alignment results of 16S rRNA and four housekeeping genes (including gyrB, recA, dnaK and rpoB) indicated the isolated strain is a member of the genus Microvirga, closely related to Microvirga lotononidis WSM3557T. In addition, results of genome average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) had revealed the lower values (ANI ≤ 88.72%, dDDH ≤ 39.5%) between strain CCABU 65841T and other related Microvirga species. The genome of the novel strain exhibits a G + C content of 64.48% and contains 7296 protein-coding genes and 93 RNA genes. The major polar lipids were found to be phosphatidylcholine and phosphatidylethanolamine. The predominant cellar fatty acids were identified to be C16:0, C18:0, C19:0 cyclo ω8c, summed feature 2, summed feature 3 and summed feature 8. Moreover, menaquinone 8 (MK-8) was detected to be the predominant quinone. Based on the phylogenetic and phenotypic dissimilarity, a novel species Microvirga calopogonii sp. nov. is proposed with the type strain CCABU 65841T (= LMG 25488 T = HAMBI 3033T).

Keywords

Microvirga Calopogonium mucunoides ANI dDDH 

Notes

Acknowledgements

This work was supported by the National Natural Science Foundation of China (Grant Nos. 31360003, 31460214, 31460181, and 31560211). We are grateful to BCCM/LMG and KCTC for providing the strains M. lupini Lut6T, M. lotononidis WSM3557T and M. pakistanensis NCCP-1258T.

Conflict of interest

The authors declare that there are no conflicts of interest.

Supplementary material

10482_2019_1285_MOESM1_ESM.pptx (188 kb)
Supplementary material 1 (PPTX 188 kb)
10482_2019_1285_MOESM2_ESM.docx (21 kb)
Supplementary material 2 (DOCX 20 kb)

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Copyright information

© Springer Nature Switzerland AG 2019

Authors and Affiliations

  1. 1.Key Laboratory for Forest Resources Conservation and Utilization in the Southwest Mountains of China, Ministry of EducationSouthwest Forestry UniversityKunmingPeople’s Republic of China
  2. 2.Key Laboratory of State Forestry Administration on Biodiversity Conservation in Southwest ChinaSouthwest Forestry UniversityKunmingPeople’s Republic of China
  3. 3.College of Biological Sciences and Rhizobium Research CenterChina Agricultural UniversityBeijingPeople’s Republic of China
  4. 4.Key Laboratory of Microbial Diversity Research and Application of Hebei Province, College of Life SciencesHebei UniversityBaodingPeople’s Republic of China

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