Serratia microhaemolytica sp. nov., isolated from an artificial lake in Southern China
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A Gram-stain negative, facultatively anaerobic, rod-shaped, non-motile and non-spore forming bacterium, designated ZS-11T, was isolated from an artificial freshwater lake in Guangzhou city, Guangdong province, China. Growth of strain ZS-11T was observed at the temperature 18–42 °C (optimum 32–37 °C), pH 6.0–8.0 (optimum 7.0) and 0.5–3.0% (w/v) NaCl (optimum 0.5%, w/v), and also found to be enhanced in the presence of CO2. Pairwise comparison of 16S rRNA gene sequences showed that the strain shared high similarities with Serratia entomophila DSM 12358T (96.1%), Serratia ficaria DSM 4569T (96.0%), Serratia plymuthica DSM 4540T (96.0%), Rahnella victoriana FRB 225T (95.9%) and Rouxiella badensis DSM 100043T (95.8%). The phylogenomic dendrograms showed that strain ZS-11T formed a distinct cluster within the clade of the genus Serratia. The major fatty acids (> 20%) present in the cells were C16:0, C16:1ω7c/C16:1ω6c and C18:1ω7c/C18:1ω6c, which were consistent with those of S. entomophila CCUG 55496T and Serratia liquefaciens CCUG 9285T. The DNA G + C content for the genome was 49.3%. Based on these phenotypic and genotypic data, strain ZS-11T is considered to represent a new species of the genus Serratia, for which the name Serratia microhaemolytica sp. nov. is proposed. The type strain is ZS-11T (= CCTCC AB 2018040T = KCTC 62413T).
KeywordsSerratia microhaemolytica sp. nov. Artificial lake Polyphasic taxonomy
P.-H. Qu was supported by Research Fund for Science and Technology Planning Project of Guangdong Province, China (2017A020215068). W.-J. Li was supported by the Guangdong Province Higher Vocational Colleges and Schools Pearl Scholar Founded Scheme (2014).
WJL and PHQ designed research and project outline. JW, WJW, and CC performed isolation, deposition, phenotypic and biochemical characteristics identification, and molecular analysis. JJ, SL and MX performed the chemotaxonomy analysis. All authors read and approved the final manuscript.
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Conflict of interest
The authors declare that they have no conflict of interest.
- Adeolu M, Alnajar S, Naushad S, Gupta RS (2016) Genome-based phylogeny and taxonomy of the ‘Enterobacteriales’: proposal for Enterobacterales ord. nov. divided into the families Enterobacteriaceae, Erwiniaceae fam. nov., Pectobacteriaceae fam. nov., Yersiniaceae fam. nov., Hafniaceae fam. nov., Morganellaceae fam. nov., and Budviciaceae fam. nov. Int J Syst Evol Microbiol 66:5575–5599CrossRefPubMedGoogle Scholar
- Aslanzadeh J (2006) Biochemical profile-based microbial identification systems. In: Tang YW, Stratton CW (eds) Advanced techniques in diagnostic microbiology. Springer, New York, pp 87–121Google Scholar
- Bankevich A, Nurk S, Antipov D, Gurevich AA, Dvorkin M, Kulikov AS, Lesin VM, Nikolenko SI, Pham S, Prjibelski AD, Pyshkin AV, Sirotkin AV, Vyahhi N, Tesler G, Alekseyev MA, Pevzner PA (2012) SPAdes: a new genome assembly algorithm and its applications to single-cell sequencing. J Comput Biol 19:455–477CrossRefPubMedPubMedCentralGoogle Scholar
- Clinical and Laboratory Standards Institute (2012) Performance standards for antimicrobial susceptibility testing. In: Twenty-eight informational supplement. CLSI Supplement document M100-S28. WayneGoogle Scholar
- Grimont G, Grimont PAD (2005) Genus XXXIV. Serratia. In: Brenner DJ, Krieg NR, Staley JT, Garrity GM (eds) Bergey’s manual of systematic bacteriology (The Proteobacteria), part B (The Gammaproteobacteria), vol 2, 2nd edn. Springer, New York, pp 799–805Google Scholar
- Kämpfer P, Nienhüser A, Packroff G, Wernicke F, Mehling A, Nixdorf K, Fiedler S, Kolauch C, Esser M (2008) Molecular identification of coliform bacteria isolated from drinking water reservoirs with traditional methods and the Colilert-18 system. Int J Hyg Environ Health 211:374–384CrossRefPubMedGoogle Scholar
- Qu PH, Deng XL, Zhang J, Chen JD, Zhang J, Zhang QX, Xiao Y, Chen SY (2009) Identification and characterization of the Francisella sp. strain 08HL01032 isolated in air condition systems. Acta Microbiol Sin 49:1003–1008Google Scholar
- Qu PH, Li Y, Salam N, Chen SY, Liu L, Gu Q, Fang BZ, Xiao M, Li M, Chen C, Li WJ et al (2016) Allofrancisella inopinata gen. nov. sp. nov. and Allofrancisella frigidaquae sp. nov. isolated from water-cooling systems and transfer of Francisella guangzhouensis Qu et al. 2013 to the new genus as Allofrancisella guangzhouensis comb. nov. Int J Syst Evol Microbiol 66:4832–4838CrossRefPubMedGoogle Scholar
- Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425Google Scholar
- Wilson K (1997) Preparation of genomic DNA from bacteria. In: Ausubel FM et al (eds) Current protocols in molecular biology. Wiley, New York, pp 2.4.1–2.4.5Google Scholar
- Xiao M, Salam N, Liu L, Jiao JY, Zheng ML, Wang J, Li S, Chen C, Li WJ, Qu PH (2018) Fastidiosibacter lacustris gen. nov., sp. nov., isolated from a lake water sample, and proposal of Fastidiosibacteraceae fam. nov. within the order Thiotrichales. Int J Syst Evol Microbiol 68:347–352CrossRefPubMedGoogle Scholar
- Zingg W, Soulake I, Baud D, Huttner B, Pfister R, Renzi G, Pittet D, Schrenzel J, Francois P (2017) Management and investigation of a Serratia marcescens, outbreak in a neonatal unit in Switzerland—the role of hand hygiene and whole genome sequencing. Antimicrob Resist Infect Control 6:125–130CrossRefPubMedPubMedCentralGoogle Scholar