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Streptomyces corynorhini sp. nov., isolated from Townsend’s big-eared bats (Corynorhinus townsendii)

  • Paris S. HammEmail author
  • Nicole A. Caimi
  • Diana E. Northup
  • Ernest W. Valdez
  • Debbie C. Buecher
  • Christopher A. Dunlap
  • David P. Labeda
  • Andrea Porras-Alfaro
Original Paper

Abstract

Four bacterial strains, with the capability of inhibiting Pseudogymnoascus destructans, the causative agent of white-nose syndrome, were isolated from male Townsend’s big-eared bats (Corynorhinus townsendii, Family: Vespertilionidae) in New Mexico. Isolates AC161, AC162, AC208, and AC230T were characterised as a novel clade using morphological, phenotypic and phylogenetic analysis. A draft genome of the type strain was completed to determine its taxonomy and secondary metabolite biosynthetic potential. Multi-locus sequence analysis nests AC230T with neighbours Streptomyces scopuliridis (NRRL B-24574T), Streptomyces lushanensis (NRRL B-24994T), Streptomyces odonnellii (NRRL B-24891T) and Streptomyces niveus (NRRL 2466T). Further phylogenetic analysis showed the MLSA distances between AC230T and its near neighbours are much greater than the generally accepted threshold (> 0.007) for bacterial species delineation. DNA–DNA relatedness between AC230T and its near neighbours ranged between 25.7 ± 2.1 and 29.9 ± 2.4%. The DNA G+C content of the genomic DNA of the type strain is 71.7 mol%. Isolate AC230T presents a white to ivory hue on most ISP media and its micromorphology exhibits ovoid spores with smooth surfaces in flexuous chains. Based on our study of AC230T, the strain warrants the assignment to a novel species, for which the name Streptomyces corynorhini sp. nov. is proposed. The type strain is AC230T (= JCM 33171T, = ATCC TSD155T).

Keywords

Actinobacteria Bat Streptomyces Taxonomy White-nose syndrome 

Notes

Acknowledgements

PSH and APA thank support from Western Illinois University and their Research Inspiring Student Excellence (RISE) and Women in Science (WIS) programs. DPL and the ARS Culture Collection CRIS project was supported by ARS National Program 301. CAD was supported by ARS National Programs 303, 304 and 306. DEN and DCB gratefully acknowledge the support of the staff at El Malpais National Monument with logistical support and assistance with the NPS Materials Transfer Agreement implementation. Any opinions, findings, conclusions, or recommendations expressed in this publication are those of the author(s) and do not necessarily reflect the view of the U.S. Department of Agriculture. The mention of firm names or trade products does not imply that they are endorsed or recommended by the USDA or USGS over other firms or similar products not mentioned. USDA is an equal opportunity provider and employer.

Author contributions

PSH Wrote the paper, Collected the data, Conceived the design; NAC Contributed to writing the paper, Collected the data; DEN Provided funding, Collected the data, Conceived the design; EWV Provided funding, Collected the data, Conceived the design; DCB Provided funding, Collected the data, Conceived the design; CAD Wrote the paper, Performed the analysis; DPL Contributed to analysis tools; APA Provided funding, Collected the data, Conceived the design.

Funding information

Initial funding was provided by the Eppley Foundation and further provided by the National Park Service through the Colorado Plateau Cooperative Ecosystem Studies unit (CPCESU) and Western National Park Association for work in El Malpais National Monument. Additional funding was provided by the IDNR (PI: Porras-Alfaro), New Mexico Game and Fish Department Share with Wildlife Program, Cave Conservancy Foundation, National Speleological Society Rapid Response Fund, and T&E, Inc. The lead author (PSH) would like to thank the Mycological Society of America for funding to present her research.

Compliance with ethical standards

Conflict of interest

The authors declare no conflicts of interests in the manuscript.

Supplementary material

10482_2019_1261_MOESM1_ESM.pdf (673 kb)
Supplementary material 1 (PDF 673 kb)

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Copyright information

© This is a U.S. government work and not under copyright protection in the U.S.; foreign copyright protection may apply 2019

Authors and Affiliations

  • Paris S. Hamm
    • 1
    • 2
    Email author
  • Nicole A. Caimi
    • 2
  • Diana E. Northup
    • 2
  • Ernest W. Valdez
    • 3
  • Debbie C. Buecher
    • 4
  • Christopher A. Dunlap
    • 5
  • David P. Labeda
    • 5
  • Andrea Porras-Alfaro
    • 1
    • 2
  1. 1.Department of Biological SciencesWestern Illinois UniversityMacombUSA
  2. 2.Department of BiologyUniversity of New MexicoAlbuquerqueUSA
  3. 3.U. S. Geological Survey, Fort Collins Science CenterFort CollinsUSA
  4. 4.Buecher Biological ConsultingTucsonUSA
  5. 5.Agriculture Research Service, US Department of AgriculturePeoriaUSA

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