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Antonie van Leeuwenhoek

, Volume 112, Issue 8, pp 1245–1252 | Cite as

Runella soli sp. nov., isolated from garden soil

  • Geeta Chhetri
  • Jiyoun Kim
  • Inhyup Kim
  • Myung Kyum Kim
  • Taegun SeoEmail author
Original Paper
  • 40 Downloads

Abstract

A Gram-stain negative, aerobic, salmon-pink, non-motile and rod-shaped bacterium, designated strain 15J11-1T, was isolated from a soil sample collected from the university garden in Nowongu, South Korea. The 16S rRNA gene sequence analysis showed that strain 15J11-1T is phylogenetically related to Runella slithyformis DSM 19594T and Runella palustris HMF3829T (96.9% and 95.4% sequence similarity, respectively). The major fatty acids of strain 15J11-1T were identified as iso-C15:0, iso-C17:0 3-OH, C16:1ω5c and summed feature 3 (C16:1ω7c and/or C16:1ω6c). The predominant respiratory quinone was identified as MK-7. The polar lipids were found to comprise of phosphatidylethanolamine, three unidentified aminolipids, five unidentified glycolipids, two unidentified aminoglycolipids, an unidentified phospholipid and an unidentified polar lipid. The G + C content in the genomic DNA of the strain 15J11-1T was determined to be 49.9 mol%. Based on the results of genotypic, phenotypic and chemotaxonomic analyses, strain 15J11-1T is concluded to represent a novel species of the genus Runella, for which the name Runella soli sp. nov. (type strain 15J11-1T = KCTC 52021T = NBRC 112817T) is proposed.

Keywords

Runella soli sp. nov Polyphasic taxonomy Cytophagaceae 16S rRNA Soil bacteria 

Notes

Acknowledgements

This work was supported by a research grant from Seoul Women’s University (2018) and the Project on Survey of Indigenous Species of Korea of the National Institute of Biological Resources (NIBR) under the Ministry of Environment (MOE) (NIBR201701206). We thank Prof Dr. Bernhard Schink (University of Konstanz, Konstanz, Germany) for the species name.

Compliance with ethical standards

Conflict of interest

The authors declare that there is no conflict of interest.

Ethical approval

This study does not describe any experimental work related to human.

Supplementary material

10482_2019_1257_MOESM1_ESM.docx (3.1 mb)
Supplementary material 1 (DOCX 3174 kb)

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Copyright information

© Springer Nature Switzerland AG 2019

Authors and Affiliations

  • Geeta Chhetri
    • 1
  • Jiyoun Kim
    • 1
  • Inhyup Kim
    • 1
  • Myung Kyum Kim
    • 2
  • Taegun Seo
    • 1
    Email author
  1. 1.Department of Life ScienceDongguk University-SeoulGoyangSouth Korea
  2. 2.Department of Bio and Environmental Technology, College of Natural ScienceSeoul Women’s UniversitySeoulSouth Korea

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