Mesorhizobium carbonis sp. nov., isolated from coal bed water
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A Gram-stain negative, aerobic, motile, short rod-shaped bacterium, designated as B2.3T, was isolated from coal bed water collected from Jincheng, Shanxi Province, China. The strain was able to grow at 10–40 °C (optimum 28–30 °C), pH 4.0–10.0 (optimum 7.0), and in the presence of 0–5.0% NaCl (optimum 3.0%, w/v). Phylogenetic analysis based on the 16S rRNA and concatenated housekeeping gene recA, atpD and glnA sequences showed strain B2.3T belongs to the genus Mesorhizobium, with Mesorhizobium oceanicum B7T as the closely related type strain. Strain B2.3T exhibited ANI value of 77.5% and GGDC value of 21.5% to M. oceanicum B7T. The major fatty acids were identified as summed feature 8 (C18:1ω7c and/or C18:1ω6c) and 11-methyl C18:1ω7c. The major polar lipids were found to consist of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine and an unidentified aminophospholipid. The predominant ubiquinone was identified as Quinone 10. Phenotypic and biochemical analysis results indicated that strain B2.3T can be distinguished from closely related type strains. On the basis of phenotypic, genotypic and chemotaxonomic characteristics, strain B2.3T is concluded to represent a novel species in the genus Mesorhizobium, for which the name Mesorhizobium carbonis sp. nov. is proposed. The type strain is B2.3T (=CGMCC 1.15730T = KCTC 52461T).
KeywordsCoal bed water Mesorhizobium carbonis MLSA Polyphasic taxonomy
Average nucleotide identity
This work was financially supported by the National Natural Science Foundation (30901108 and 21776310), the Shandong Provincial Natural Science Foundation (ZR2009EQ007 and ZR2017MB019) and the Fundamental Research Funds for the Central Universities (15CX02014A and 16CX02044A).
JL designed the study and wrote the manuscript. WX, ZZX and FQX performed the experiments. JL and LJX analyzed the data. JJZ contributed to the polar lipid analysis. JBQ and JGL revised the manuscript.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
This article does not contain any studies with human participants or animals performed by any of the authors.
- Collins MD, Dorothy J (1980) Lipids in the classification and identification of coryneform bacteria containing peptidoglycans based on 2, 4-diaminobutyric acid. J Appl Microb 48:459–470Google Scholar
- De Meyer SE, Tan HW, Andrews M, Heenan PB, Willems A (2016) Mesorhizobium calcicola sp. nov., Mesorhizobium waitakense sp. nov., Mesorhizobium sophorae sp. nov., Mesorhizobium newzealandense sp. nov. and Mesorhizobium kowhaii sp. nov. isolated from Sophora root nodules. Int J Syst Evol Microbiol 66:786–795CrossRefGoogle Scholar
- Jarvis B, Van Berkum P, Chen W, Nour S, Fernandez M, Cleyet-Marel J, Gillis M (1997) Transfer of Rhizobium loti, Rhizobium huakuii, Rhizobium ciceri, Rhizobium mediterraneum, and Rhizobium tianshanense to Mesorhizobium gen. nov. Int J Syst Evol Microbiol 47:895–898Google Scholar
- Küster E (1959) Outline of a comparative study of criteria used in characterization of the actinomycetes. Int J Syst Evol Microbiol 9:97–104Google Scholar
- Marmur J (1961) A procedure for the isolation of deoxyribonucleic acid from micro-organisms. J Mol Biol 3:208-IN201Google Scholar
- Martínez-Hidalgo P, Ramírez-Bahena MH, Flores-Félix JD, Rivas R, Igual JM, Mateos PF, Martínez-Molina E, León-Barrios M, Peix A, Velázquez E (2015) Revision of the taxonomic status of type strains of Mesorhizobium loti and reclassification of strain USDA 3471T as the type strain of Mesorhizobium erdmanii sp. nov. and ATCC 33669T as the type strain of Mesorhizobium jarvisii sp. nov. Int J Syst Evol Microbiol 65:1703–1708CrossRefGoogle Scholar
- Minnikin D, Collins M, Goodfellow M (1979) Fatty acid and polar lipid composition in the classification of Cellulomonas, Oerskovia and related taxa. J Appl Microb 47:87–95Google Scholar
- Mohamad R, Willems A, Le Quere A, Maynaud G, Pervent M, Bonabaud M, Dubois E, Cleyet-Marel JC, Brunel B (2017) Mesorhizobium delmotii and Mesorhizobium prunaredense are two new species containing rhizobial strains within the symbiovar anthyllidis. Syst Appl Microbiol 40:135–143CrossRefGoogle Scholar
- Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425Google Scholar
- Sasser M (1990) Identification of bacteria by gas chromatography of cellular fatty acids. MIDI Technical Note 101 MIDI IncGoogle Scholar
- Smibert R, Krieg N (1994) Phenotypic characterization. In: Gerhardt P, Murray RGE, Wood WA, Krieg NR (eds) Methods for general and molecular bacteriology. American Society for Microbiology, Washington, pp 607–654Google Scholar
- Tighe S, De Lajudie P, Dipietro K, Lindström K, Nick G, Jarvis B (2000) Analysis of cellular fatty acids and phenotypic relationships of Agrobacterium, Bradyrhizobium, Mesorhizobium, Rhizobium and Sinorhizobium species using the Sherlock Microbial Identification System. Int J Syst Evol Microbiol 50:787–801CrossRefGoogle Scholar